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Electrophysiological and molecular characterization of neurons involved in the generation of respiratory rhythm and pattern of rats during postnatal development

Abstract

The respiratory rhythm and respiratory pattern of three phases (inspiration, post-inspiration and second half of expiration) are generated by a neural network located in the ventral surface of the medulla, coordinated by synaptic interactions, specifically in the Parafacial Respiratory Group (pFRG), Bötzinger Complex (BötC) and the pre-Bötzinger Complex. (pre-BötC). Synaptic interactions between the neurons involved in the generation of respiratory rhythm and pattern in the ventral surface of the medulla have been the subject of intense research in the field of central control of respiration. However, the electrophysiological and molecular mechanisms of each neuron involved in the generation of respiratory rhythm and pattern are not fully understood and is one of the goals of this project. Furthermore, little is known about changes in electrophysiological and molecular mechanisms of different respiratory neurons during postnatal development, mechanisms that could explain the changes in respiratory rhythm and pattern in the different phases of the development. Thus, the aims of this project are to investigate the electrophysiological properties of different respiratory neurons of the ventral surface of medulla (pFRG, BötC and pre-BötC), the expression of voltage-dependent and leak channels that determine such electrophysiological properties and the morphology of these neurons during the postnatal development (P0-P40). The results to be obtained from this project, using advanced techniques of electrophysiology and molecular biology, will provide important functional information of how intrinsic electrophysiological properties and molecular profile of each group of respiratory neurons of the ventral surface of the medulla contribute to their firing patterns and consequently to the generation of respiratory rhythm and pattern during the postnatal development. (AU)

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Scientific publications (16)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MORAES, DAVI J. A.; BONAGAMBA, LENI G. H.; DA SILVA, MELINA P.; PATON, JULIAN F. R.; MACHADO, BENEDITO H.. Role of ventral medullary catecholaminergic neurons for respiratory modulation of sympathetic outflow in rats. SCIENTIFIC REPORTS, v. 7, . (13/06077-5, 15/01073-7, 13/10484-5)
MAGALHAES, KAROLYNE S.; SPILLER, PEDRO F.; DA SILVA, MELINA P.; KUNTZE, LUCIANA B.; PATON, JULIAN F. R.; MACHADO, BENEDITO H.; MORAES, DAVI J. A.. Locus Coeruleus as a vigilance centre for active inspiration and expiration in rats. SCIENTIFIC REPORTS, v. 8, . (13/10484-5, 15/01073-7, 17/09071-9, 16/24869-4, 13/06077-5)
PIJACKA, WIOLETTA; MORAES, DAVI J. A.; RATCLIFFE, LAURA E. K.; NIGHTINGALE, ANGUS K.; HART, EMMA C.; DA SILVA, MELINA P.; MACHADO, BENEDITO H.; MCBRYDE, FIONA D.; ABDALA, ANA P.; FORD, ANTHONY P.; et al. Purinergic receptors in the carotid body as a new drug target for controlling hypertension. Nature Medicine, v. 22, n. 10, p. 1151-1159, . (13/06077-5, 13/10484-5)
MORAES, DAVI J. A.; MACHADO, BENEDITO H.; PATON, JULIAN F. R.. Carotid body overactivity induces respiratory neurone channelopathy contributing to neurogenic hypertension. JOURNAL OF PHYSIOLOGY-LONDON, v. 593, n. 14, p. 3055-3063, . (13/06077-5, 13/10484-5)
MORAES, DAVI J. A.; DA SILVA, MELINA P.; SPILLER, PEDRO F.; MACHADO, BENEDITO H.; PATON, JULIAN F. R.. Purinergic plasticity within petrosal neurons in hypertension. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, v. 315, n. 5, p. R963-R971, . (16/24869-4, 13/06077-5, 15/01073-7, 13/10484-5)
DA SILVA, MELINA P.; MORAES, DAVI JOSE A.; BONAGAMBA, LENI G. H.; MECAWI, ANDRE DE SOUZA; VARANDA, WAMBERTO A.; MACHADO, BENEDITO H.. Hyperexcitability and plasticity induced by sustained hypoxia on rectus abdominis motoneurons. JOURNAL OF PHYSIOLOGY-LONDON, v. 597, n. 7, p. 1935-1956, . (13/06077-5, 13/10484-5)
DE BRITTO, ALAN A.; MORAES, DAVI J. A.. Non-chemosensitive parafacial neurons simultaneously regulate active expiration and airway patency under hypercapnia in rats. JOURNAL OF PHYSIOLOGY-LONDON, v. 595, n. 6, p. 2043-2064, . (13/10484-5)
MORAES, DAVI J. A.; BONAGAMBA, LENI G. H.; DA SILVA, MELINA P.; MECAWI, ANDRE S.; ANTUNES-RODRIGUES, JOSE; MACHADO, BENEDITO H.. Respiratory Network Enhances the Sympathoinhibitory Component of Baroreflex of Rats Submitted to Chronic Intermittent Hypoxia. Hypertension, v. 68, n. 4, p. 1021-1030, . (13/06077-5, 13/10484-5)
DA SILVA, MELINA PIRES; DE ALMEIDA MORAES, DAVI JOSE; MECAWI, ANDRE DE SOUZA; RODRIGUES, JOSE ANTUNES; VARANDA, WAMBERTO ANTONIO. Nitric Oxide Modulates HCN Channels in Magnocellular Neurons of the Supraoptic Nucleus of Rats by an S-Nitrosylation-Dependent Mechanism. JOURNAL OF NEUROSCIENCE, v. 36, n. 44, p. 11320-11330, . (12/19750-7, 13/10484-5)
ROLOFF, EVA V. L.; WALAS, DAWID; MORAES, DAVI J. A.; KASPAROV, SERGEY; PATON, JULIAN F. R.. Differences in autonomic innervation to the vertebrobasilar arteries in spontaneously hypertensive and Wistar rats. JOURNAL OF PHYSIOLOGY-LONDON, v. 596, n. 16, p. 3505-3529, . (13/10484-5)
SPILLER, PEDRO F.; DA SILVA, MELINA P.; MORAES, DAVI J. A.. Lactate does not activate the carotid body of Wistar rat. Respiratory Physiology & Neurobiology, v. 285, . (16/24869-4, 18/07027-5, 13/10484-5)
DE BRITTO, ALAN A.; MAGALHAES, KAROLYNE S.; DA SILVA, MELINA P.; PATON, JULIAN F. R.; MORAES, DAVI J. A.. Active expiratory oscillator regulates nasofacial and oral motor activities in rats. Experimental Physiology, v. 105, n. 2, . (15/01073-7, 13/10484-5, 17/09071-9, 16/24994-3)
MAGALHAES, KAROLYNE S.; DE BRITTO, ALAN A.; PATON, JULIAN F. R.; MORAES, DAVI J. A.. A6 neurons simultaneously modulate active expiration and upper airway resistance in rats. Experimental Physiology, v. 105, n. 1, p. 53-64, . (16/24994-3, 17/09071-9, 13/10484-5)
CASTANIA, JACI A.; KATAYAMA, PEDRO L.; BROGNARA, FERNANDA; MORAES, DAVI J. A.; SABINO, JOAO PAULO J.; SALGADO, HELIO C.. Selective denervation of the aortic and carotid baroreceptors in rats. Experimental Physiology, v. 104, n. 9, p. 1335-1342, . (17/05163-6, 13/10484-5, 13/20549-7, 15/18511-7)
ZOCCAL, DANIEL B.; COLOMBARI, DEBORA S. A.; COLOMBARI, EDUARDO; FLOR, KARINE C.; DA SILVA, MELINA P.; COSTA-SILVA, JOAO H.; MACHADO, BENEDITO H.; MORAES, DAVI J. A.; MURPHY, DAVID; PATON, JULIAN F. R.. Centrally acting adrenomedullin in the long-term potentiation of sympathetic vasoconstrictor activity induced by intermittent hypoxia in rats. Experimental Physiology, v. 104, n. 9, p. 1371-1383, . (15/23467-7, 13/06077-5, 13/10484-5)
MORAES, DAVI J. A.; MACHADO, BENEDITO H.. Electrophysiological properties of laryngeal motoneurones in rats submitted to chronic intermittent hypoxia. JOURNAL OF PHYSIOLOGY-LONDON, v. 593, n. 3, p. 619-634, . (13/06077-5, 13/10484-5)

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