Participation of lactate and carotid bodies in the respiratory responses of rats submitted to anaerobic physical exercise

• Grant number: 16/24869-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): May 01, 2017
• Effective date (End): December 31, 2018
• Field of knowledge: Biological Sciences - Biophysics - Cellular Biophysics
• Principal Investigator: Davi José de Almeida Moraes
• Grantee: Pedro Favoretto Spiller
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 13/10484-5 - Electrophysiological and molecular characterization of neurons involved in the generation of respiratory rhythm and pattern of rats during postnatal development, AP.JP

Abstract

Peripheral chemoreceptors located in the carotid sinus or Carotid Body are structures formed by type I Glomus cells, which are activated in response to reductions in oxygen partial pressure (hypoxia) in arterial blood. After depolarization of these cells, there is an increase in ventilation, by inspiratory and expiratory responses, as well as autonomic and behavioral responses. During hypoxic conditions lactate produced by anaerobic respiration seems to be responsible for stimulating the type I Glomus cells of Carotid Body, increasing intracellular calcium levels and the activity of carotid sinus nerve, which transmit the information to respiratory nuclei located in the brainstem. Thus, the lactate is supposed to act as an indicator of the metabolic state of tissues, regulating, thereby, respiratory responses in conditions of metabolic challenges. Therefore, the hypothesis of the present study is the following: the increase in lactate concentration in arterial blood of rats in response to anaerobic exercise modifies the activity of type I Glomus cells of Carotid Body and contributes to the respiratory responses during exercise. The aims of the present study are: (I) to evaluate whether the increase in plasma lactate concentration can promote inspiratory and expiratory responses in rats submitted to anaerobic exercise by activation of peripheral chemoreceptors, and; (II) to evaluate whether the lactate changes the electrophysiological properties and intracellular calcium concentration in type I Glomus cells of Carotid Body of rats. With this study we will improve our knowledge about the contribution of lactate for respiratory responses to physical exercise, and how lactate can modify the electrophysiological properties of type I Glomus cells of Carotid Body of rats. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SPILLER, PEDRO F.; DA SILVA, MELINA P.; MORAES, DAVI J. A.. Lactate does not activate the carotid body of Wistar rat. Respiratory Physiology & Neurobiology, v. 285, MAR 2021. (16/24869-4, 18/07027-5, 13/10484-5)

MORAES, DAVI J. A.; DA SILVA, MELINA P.; SPILLER, PEDRO F.; MACHADO, BENEDITO H.; PATON, JULIAN F. R.. Purinergic plasticity within petrosal neurons in hypertension. AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, v. 315, n. 5, p. R963-R971, NOV 2018. (15/01073-7, 13/06077-5, 16/24869-4, 13/10484-5)

SPILLER, PEDRO F.; DA SILVA, CARLOS A. A.; FRANCESCATO, HELOISA D. C.; MORAES, DAVI J. A.. The role of carotid bodies in the generation of active inspiratory and expiratory responses to exercise in rats. Experimental Physiology, v. 105, n. 8, MAY 2020. (16/24869-4)

MAGALHAES, KAROLYNE S.; SPILLER, PEDRO F.; DA SILVA, MELINA P.; KUNTZE, LUCIANA B.; PATON, JULIAN F. R.; MACHADO, BENEDITO H.; MORAES, DAVI J. A.. Locus Coeruleus as a vigilance centre for active inspiration and expiration in rats. SCIENTIFIC REPORTS, v. 8, OCT 23 2018. (13/10484-5, 15/01073-7, 17/09071-9, 16/24869-4, 13/06077-5)