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Functional, biochemical and molecular study of P1 and P2 purinergic receptors present in the heart from genetically hypertensive rats

Grant number: 13/20402-6
Support Opportunities:Regular Research Grants
Start date: August 01, 2014
End date: July 31, 2016
Field of knowledge:Biological Sciences - Pharmacology - General Pharmacology
Principal Investigator:Aron Jurkiewicz
Grantee:Aron Jurkiewicz
Host Institution: Instituto Nacional de Farmacologia (INFAR). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated researchers:Neide Hyppolito Jurkiewicz

Abstract

The purine nucleotides, as ATP and pyrimidine, as UTP, are stored together with noradrenaline in synaptic vesicles, being released in nerve terminals. Their action is mediated by purinergic receptors P1 and P2, expressed in various tissues. The purinergic system is known to be involved in cardiovascular regulation. Both nucleotides stimulate vasoconstriction and dilatation, growing of vascular and endothelial cells, angiogenesis and vascular remodeling. Since cellular and molecular mechanisms involving purinoceptors and heart are not fully clarified in systemic hypertension, we found it important to investigate the role of these receptors in normotensive and hypertensive rats. For this purpose, we will isolate the right and left atria as well as the ventricle in Normal, Wistar Kyoto and SHR rats, 1 to 6 months old. Functional, biochemical and molecular essays will be performed to investigate whether P1 and P2 receptors signalization is modified in hypertension. Therefore, selective agonists and antagonists will be used to characterize the purinergic receptors in regulation of cardiac function. In addition a functional study of calcium transients in inotropic positive effects of ATP and UTP in atria from normal and hypertensive rats will be performed. Real time PCR technique will be used to quantify the receptors expression differences in SHR, and through the Western blotting and immunohistochemistry we will study the presence and production of these receptors. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RODRIGUES, JULIANO Q. D.; CAMARA, HENRIQUE; JURKIEWICZ, ARON; GODINHO, ROSELY O.. Increased Gi protein signaling potentiates the negative chronotropic effect of adenosine in the SHR right atrium. NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, v. 391, n. 5, p. 513-522, . (13/20402-6, 12/22763-3, 15/07019-4, 12/24828-5)
CAMARA, HENRIQUE; DA SILVA JUNIOR, EDILSON DANTAS; GARCIA, ANTONIO G.; JURKIEWICZ, ARON; DANTAS RODRIGUES, JULIANO QUINTELLA. Cardiac arrest induced by muscarinic or adenosine receptors agonists is reversed by DPCPX through double mechanism. European Journal of Pharmacology, v. 819, p. 9-15, . (13/20402-6)
CAMARA, HENRIQUE; DANTAS RODRIGUES, JULIANO QUINTELLA; ALVES, GABRIEL ANDRADE; DA SILVA JUNIOR, EDILSON DANTAS; CARICATI-NETO, AFONSO; GARCIA, ANTONIO G.; JURKIEWICZ, ARON. Would calcium or potassium channels be responsible for cardiac arrest produced by adenosine and ATP in the right atria of Wistar rats?. European Journal of Pharmacology, v. 768, p. 199-206, . (13/20402-6)