Molecular marker assisted selection using the AB-QTL method and genotyping by sequencing (GBS) toward angular leaf spot and antracnose resistance in common beans

• Grant number: 14/11145-2
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2014
• End date: December 31, 2017
• Field of knowledge: Agronomical Sciences - Agronomy - Crop Science
• Principal Investigator: Luciana Lasry Benchimol-Reis
• Grantee: Luciana Lasry Benchimol-Reis
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated researchers: Alisson Fernando Chiorato ; Sérgio Augusto Morais Carbonell

Abstract

Among the common bean diseases of the, the angular leaf spot (ALS) and anthracnose (ANT) cause reduction in the yield and the quality of grain produced, and due to the great variability of these pathogens, a durable resistance of many cultivars was not achieved. A large number of molecular markers were associated with QTLs for resistance to this disease by our group of research. According to studies of our research group, ALS and ANT showed quantitative inheritance and several QTLs should be introgressed in order to produce a lasting resistance to different races of the pathogen. The AB-QTL (Advanced backcross QTL Analysis) technique is able to select, introgress and simultaneously map the QTLs, through backcrossing, and therefore the effects (epistatic and pleiotropic) of the combination of different QTLs can be measured, and only the QTLs favorable are transferred, without losing the quality of elite cultivar used as the recurrent parent. While the other methods of assisted selection are based on first performing the mapping of the QTLs for then transferring those that are favorable, the AB-QTL method suggests evaluating simultaneously the families and/or progenies that phenotypically superior together with the introgression of QTLs, reducing the problem of linkage drag and negative interactions. Phenotypic selection eliminates the progenies with lower performance; even those that have favorable QTLs introgressed, but can have their values changed by genetic epistatic, pleiotropic effects or by genotype x environment interactions. The objective of this project is to use the technique of AB-QTL to promote a gain on the genetic resistance to ALS in common bean breeding program of the Agronomic Institute of Campinas (IAC). For ALS, the 'IAC Milênio' cultivar (susceptible, recurrent parental) will be crossed with the 'AND 277' cultivar (resistant, parental donor) while for ANT, the 'Pérola' (susceptible, recurrent parent) cultivar will be crossed to the 'IAC-Formoso' (resistant, donor parent) cultivar. Phenotypical selection of plants that have desirable agronomical patterns for common bean will be made in RC1 and RC2, and evaluation with molecular markers in large scale will occur in F2BC22. In this generation, the QTLs will be mapped using GBS, useful for the detection and selection of a high number of SNP markers, and near isogenic lines (QTL-NILs) will be evaluated. The disease phenotyping will be taken in the greenhouse during mapping, but then the selection will be conducted in experimental field conditions with natural infection. It is expected that new cultivars may be recommended for the common bean breeding program of the IAC and that they show effective resistance in harvesting conditions. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE ALMEIDA, CALEO PANHOCA; ARRUDA, NATALIA; DE CARVALHO PAULINO, JEAN FAUSTO; DE FREITAS, GABRIEL MAMEDIO; JANINI BONFANTE, GABRIEL FRANCESCO; BAJAY, MIKLOS MAXIMILIANO; DE DEUS, BIANCA CRISTINA; ALVES PATRICIO, FLAVIA RODRIGUES; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; et al. Genetic diversity ofPseudocercospora griseolaresistancelociin common beans. TROPICAL PLANT PATHOLOGY, v. 46, n. 1, SEP 2020. (17/01753-3, 14/19967-1, 14/11145-2)

ALMEIDA, CALEO PANHOCA DE; DE CARVALHO PAULINO, JEAN FAUSTO; BONFANTE, GABRIEL FRANCESCO JANINI; PERSEGUINI, JULIANA MORINI KUPPER CARDOSO; SANTOS, ISABELLA LAPORTE; GONCALVES, JOAO GUILHERME RIBEIRO; PATRICIO, FLAVIA RODRIGUES ALVES; TANIGUTI, CRISTIANE HAYUMI; GESTEIRA, GABRIEL DE SIQUEIRA; GARCIA, ANTONIO AUGUSTO FRANCO; et al. Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean. FRONTIERS IN PLANT SCIENCE, v. 12, APR 13 2021. (18/20992-1, 18/15526-1, 19/19670-2, 14/11145-2, 17/24711-4, 17/01753-3, 14/08280-5)

DE ALMEIDA, CALEO PANHOCA; PAULINO, JEAN FAUSTO DE CARVALHO; SANTOS, ISABELLA LAPORTE; BAJAY, MIKLOS MAXIMILIANO; GONCALVES, JOAO GUILHERME RIBEIRO; CARVALHO, CASSIA REGINA LIMONTA; CARBONELL, SERGIO AUGUSTO MORAIS; CHIORATO, ALISSON FERNANDO; BENCHIMOL-REIS, LUCIANA LASRY. Marker-assisted backcrossing for disease resistance and agronomic traits in Carioca beans. CROP SCIENCE, v. 61, n. 4, p. 2510-2521, JUL 2021. (14/11145-2, 17/01753-3)

DE CARVALHO PAULINO, JEAN FAUSTO; DE ALMEIDA, CALEO PANHOCA; SANTOS, ISABELLA LAPORTE; RIBEIRO GONCALVES, JOAO GUILHERME; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; BENCHIMOL-REIS, LUCIANA LASRY. Combining disease resistance and postharvest quality traits by early marker-assisted backcrossing in carioca beans. Scientia Agricola, v. 79, n. 2, 2022. (14/11145-2, 17/01753-3)

DE ALMEIDA, CALEO PANHOCA; ARRUDA, NATALIA; DE CARVALHO PAULINO, JEAN FAUSTO; DE FREITAS, GABRIEL MAMEDIO; JANINI BONFANTE, GABRIEL FRANCESCO; BAJAY, MIKLOS MAXIMILIANO; DE DEUS, BIANCA CRISTINA; ALVES PATRICIO, FLAVIA RODRIGUES; MORAIS CARBONELL, SERGIO AUGUSTO; CHIORATO, ALISSON FERNANDO; et al. Genetic diversity ofPseudocercospora griseolaresistancelociin common beans. ROPICAL PLANT PATHOLOG, v. 46, n. 1, p. 10-pg., 2020-09-03. (14/19967-1, 14/11145-2, 17/01753-3)