Gene expression analysis of tissue health in subjects with clinical and subclinical thyroid dysfunction

• Grant number: 14/04193-0
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2014
• End date: January 31, 2017
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Cléber Pinto Camacho
• Grantee: Cléber Pinto Camacho
• Host Institution: Universidade Nove de Julho (UNINOVE). Campus Vergueiro. São Paulo , SP, Brazil
• Associated researchers: Humberto Dellê ; Ivone da Silva Duarte

Abstract

The tissue health is critical to the patients' clinical management. In the last years, the increment of the molecular biology knowledge reveled the exosomes as a noninvasive diagnostic strategy with power to analyze the tissue physiology. RNA detection with diagnostic purpose can overcome the traditional methods. Although less controversy follows the hypothyroidism treatment, the real need of T3 reposition with T4 dilemma and the subclinical disease controversy persist. Many steps are necessary to the thyroid hormone actions and any modification of this complex system can alter the T3 tissue response. Altered expression of 16 genes was found in blood samples of Graves Disease patients. Our goal is to identify differential expressed genes in healthy people and in hypothyroidism patients. Exosomes will be extracted and analyzed through whole genomic expression chip. The differential expressed genes will be validated in silica and in vivo. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE LIMA HIRATA, ANDREA HARUMY; DE JESUS ROCHA, LUIZ ANTONIO; DA SILVA, VALDELENA ALESSANDRA; DE ALMEIDA, ROBSON JOSE; BACIGALUPO, LUCAS DOS SANTOS; VARELA, PATRICIA; MARTINS, LEONARDO; PESQUERO, JOAO BOSCO; DELLE, HUMBERTO; CAMACHO, CLEBER PINTO. Circulating RNA Transcriptome of Pregnant Women with TSH Just Above the Trimester-Specific Reference and its Correlation with the Hypertensive Phenotype. SCIENTIFIC REPORTS, v. 10, n. 1, APR 15 2020. (14/04193-0)

HIRATA, ANDREA HARUMY DE LIMA; ROCHA, LUIZ ANTONIO DE JESUS; SILVA, VALDELENA ALESSANDRA; DE ALMEIDA, ROBSON JOSE; RODRIGUES, ALBERTINA GOMES; DELLE, HUMBERTO; CAMACHO, CLEBER P.. DESCRIPTION OF DIFFERENTIALLY EXPRESSED GENES BETWEEN HEALTHY NON-PREGNANT AND PREGNANT WOMEN IN AN RNAseq TRANSCRIPTOME STUDY. FASEB JOURNAL, v. 32, n. 1, p. 2-pg., 2018-04-01. (14/04193-0)

DA SILVA, VALDELENA ALESSANDRA; DE ALMEIDA, ROBSON JOSE; CAVALCANTE, MARCELE PIMENTA; PEREIRA JUNIOR, LUCIO ANTONIO; REIS, FRANCINE MACHADO; PEREIRA, MARIANA FABBRIS; KASAMATSU, TERESA S.; CAMACHO, CLEBER P.. Two Thyroid Stimulating Hormone assays correlated in clinical practice show disagreement in subclinical hypothyroidism patients. CLINICAL BIOCHEMISTRY, v. 53, p. 13-18, MAR 2018. (14/04193-0)

ROBSON JOSÉ DE ALMEIDA; ANDRÉA HARUMY DE LIMA HIRATA; LUIZ ANTÔNIO DE JESUS ROCHA; MIRIAM DUARTE DE ARRUDA MOTTA; PATRICIA VARELA; LEONARDO MARTINS; JOÃO BOSCO PESQUERO; CLÉBER P. CAMACHO. Similar hypothyroid and sepsis circulating mRNA expression could be useful as a biomarker in nonthyroidal illness syndrome: a pilot study. ARCHIVES OF ENDOCRINOLOGY METABOLISM, v. 67, n. 5, 2023-06-05. (14/04193-0)