Iodinated contrast induced acute kidney injury in diabetic and nephropathy diabetic in rats

• Grant number: 13/26560-2
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2014
• End date: October 31, 2016
• Field of knowledge: Health Sciences - Nursing - Medical-Surgical Nursing
• Principal Investigator: Maria de Fatima Fernandes Vattimo
• Grantee: Maria de Fatima Fernandes Vattimo
• Host Institution: Escola de Enfermagem (EE). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Cassiane Dezoti da Fonseca ; Fernanda Teixeira Borges ; Mirian Watanabe

Abstract

The contrast-induced nephropathy (CIN) is directly linked to chronic diseases that compromise the oxygenation in the region of the renal medulla. Chronic hyperglycemia changes a hemodynamic in the microvasculature, endothelial dysfunction and activation of inflammatory processes and oxidative injury, incurring decreased in glomerular filtration rate (GFR). The aim of this study is to investigate the NIC at high risk factor such as diabetes mellitus (DM) and preexisting chronic renal disease and elucidate the role of the endothelial injury and renal microvasculature in this injury model. Material and Methods: Adult male Wistar rats and tubular cells, HK-2 e IRPTC, will be used. DM will be induced by streptozotocin and 5/6 renal ablation model (Nx) wil be performed. Cells will be cultured in hyperglycemic media. Renal function by inulin clearance and microalbuminuria, oxidative stress, expression and quantification of inducible nitric oxide synthase (iNOS), HO-1, HO-2, histology of renal tissue, analysis of genic expression of HO-1, HO-2 and synthase NO, quantification of NO, HK-2 and IRPTC cells transfection with siRNA to HO-1 and with plasmid for HO-1 will be performed. (AU)

Scientific publications

(12)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, SHEILA MARQUES; MARTINS, DANIEL MALISANI; DA FONSECA, CASSIANE DEZOTI; WATANABE, MIRIAN; FERNANDES VATTIMO, ANDMARIA DE FATIMA. Impact of Iodinated Contrast on Renal Function and Hemodynamics in Rats with Chronic Hyperglycemia and Chronic Kidney Disease. BIOMED RESEARCH INTERNATIONAL, 2016. (13/26560-2)

FERNANDES, SHEILA MARQUES; CORDEIRO, PRISCILLA MENDES; WATANABE, MIRIAN; DA FONSECA, CASSIANE DEZOTI; FERNANDES VATTIMO, MARIA DE FATIMA. The role of oxidative stress in streptozotocin-induced diabetic nephropathy in rats. ARCHIVES OF ENDOCRINOLOGY METABOLISM, v. 60, n. 5, p. 443-449, OCT 2016. (11/24028-6, 13/26560-2)

SCHLOTTFELDT, FABIO DOS SANTOS; FERNANDES, SHEILA MARQUES; MARTINS, DANIEL MALISANI; CORDEIRO, PRISCILLA; DA FONSECA, CASSIANE DEZOTI; WATANABE, MIRIAN; FERNANDES VATTIMO, MARIA DE FATIMA. Prevention of amphotericin B nephrotoxicity through use of phytotherapeutic medication. Revista da Escola de Enfermagem da USP, v. 49, n. SI, p. 73-78, FEB 2015. (11/24028-6, 13/26560-2)

FERNANDES, SHEILA MARQUES; MARTINS, DANIEL MALISANI; DA FONSECA, CASSIANE DEZOTI; WATANABE, MIRIAN; FERNANDES VATTIMO, ANDMARIA DE FATIMA. Impact of Iodinated Contrast on Renal Function and Hemodynamics in Rats with Chronic Hyperglycemia and Chronic Kidney Disease. BIOMED RESEARCH INTERNATIONAL, v. 2016, p. 5-pg., 2016-01-01. (13/26560-2)

CORDEIRO, PRISCILLA MENDES; FERNANDES, SHEILA MARQUES; DA FONSECA, CASSIANE DEZOTI; WATANABE, MIRIAN; LOPES, SERGIO MARTINS; FERNANDES VATTIMO, MARIA DE FATIMA. Efeito da Justicia acuminatissima, Sara Tudo do Amazonas, na injúria renal aguda isquêmica: estudo experimental. Revista da Escola de Enfermagem da USP, v. 53, 2019. (11/24028-6, 13/26560-2)

FERNANDES VATTIMO, MARIA DE FATIMA; WATANABE, MIRIAN; DA FONSECA, CASSIANE DEZOTI; DE MOURA NEIVA, LUCIANA BARROS; PESSOA, EDSON ANDRADE; BORGES, FERNANDA TEIXEIRA. Polymyxin B Nephrotoxicity: From Organ to Cell Damage. PLoS One, v. 11, n. 8, AUG 17 2016. (11/24028-6, 13/26560-2)

VASCO, CAROLINA FERREIRA; WATANABE, MIRIAN; DA FONSECA, CASSIANE DEZOTI; FERNANDES VATTIMO, MARIA DE FATIMA. Lesão renal aguda induzida pela sepse: efeito de proteção renal dos antioxidantes. Revista Brasileira de Enfermagem, v. 71, n. 4, p. 1921-1927, JUL-AUG 2018. (11/24028-6, 13/26560-2)

CHIMIRRI DE LIMAS MARTINS, ANNA LUIZA; WATANABE, MIRIAN; FERNANDES, SHEILA MARQUES; DA FONSECA, CASSIANE DEZOTI; FERNANDES VATTIMO, MARIA DE FATIMA. Diabetes Mellitus: fator de risco para toxicidade de medicamentos. Revista da Escola de Enfermagem da USP, v. 52, 2018. (11/24028-6, 13/26560-2)

DA FONSECA, CASSIANE DEZOTI; WATANABE, MIRIAN; FERNANDES COUTO, SHEILA MARQUES; CARNEIRO DOS SANTOS, ALEF ARAGAO; BORGES, FERNANDA TEIXEIRA; FERNANDES VATTIMO, MARIA DE FATIMA. The renoprotective effects of Heme Oxygenase-1 during contrast-induced acute kidney injury in pre-clinical diabetic models. Clinics, v. 76, 2021. (11/24028-6, 13/26560-2)

CASSIANE DEZOTI DA FONSECA; MIRIAN WATANABE; SHEILA MARQUES FERNANDES COUTO; ALEF ARAGÃO CARNEIRO DOS SANTOS; FERNANDA TEIXEIRA BORGES; MARIA DE FATIMA FERNANDES VATTIMO. The renoprotective effects of Heme Oxygenase-1 during contrast-induced acute kidney injury in preclinical diabetic models. Clinics, v. 76, 2021-10-18. (13/26560-2, 11/24028-6)

FÁBIO DOS SANTOS SCHLOTTFELDT; SHEILA MARQUES FERNANDES; DANIEL MALISANI MARTINS; PRISCILLA CORDEIRO; CASSIANE DEZOTI DA FONSECA; MIRIAN WATANABE; MARIA DE FATIMA FERNANDES VATTIMO. Prevenção da nefrotoxicidade da anfotericina B por meio do uso de fitomedicamentos. Revista da Escola de Enfermagem da USP, v. 49, p. 74-79, 2015-12-00. (13/26560-2, 11/24028-6)

DE ANDRADE COELHO, FILIPE UTUARI; WATANABE, MIRIAN; DA FONSECA, CASSIANE DEZOTI; PADILHA, KATIA GRILLO; FERNANDES VATTIMO, MARIA DE FATIMA. Nursing Activities Score e a lesão renal aguda. Revista Brasileira de Enfermagem, v. 70, n. 3, p. 475-480, MAY-JUN 2017. (13/26560-2)