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Sperm DNA fragmantation in the lipid profile of murine blastocyst

Grant number: 14/08971-8
Support type:Regular Research Grants
Duration: September 01, 2014 - February 28, 2017
Field of knowledge:Health Sciences - Medicine
Principal researcher:Agnaldo Pereira Cedenho
Grantee:Agnaldo Pereira Cedenho
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Assoc. researchers:Edson Guimarães Lo Turco

Abstract

Heat stress has a significant impact in sperm DNA fragmentation. The fertilization of healthy oocytes with high DNA fragmentation sperm may result in poor embryo quality, decrease the implantation rate and spontaneous abortions. It is known the intimate relationship between lipids and metabolic process in the fertilization, membrane fusion and decondensation of cortical granules. However is not well established how the lipids profile can be associated with embryonic development to the blastocyst and the performance of sperm DNA fragmentation in the embryo development. The aim of this project is compare the lipid profile of mouse embryos derived from spermatozoa with and without DNA fragmentation induced by heat stress. In this study, FVB/NJ and C57BL/6 mice will used as model to study the impact of sperm DNA fragmentation in the development and lipid profile of blastocysts. The groups will formed by in vitro fertilization (IVF), in vivo fertilization, collected in zygote phase and submitted to in vitro culture until to the blastocyst phase (ZIV) and in vivo fertilization collecting in the blastocyst phase (BIV), each group will have the control (C) and DNA fragmentation experiment (DNA F) . Lipid analysis of the embryos will performed using the Matrix- Assisted Laser Desorption Ionization - Time of Flight /Time of Flight (MALDI TOF/TOF) then the blastocysts are stained with Hoechst 33342 and propidium iodide to analyze the number of cells and embryo quality. (AU)

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