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Study of kinetic behavior of convertases

Abstract

The protein-convertases (PCs) are proteolytic enzymes that belong to the family of subtilisin-like serine proteases. These enzymes are found in the secretory pathways and in secretory vesicles of trans-Golgi in the eukaryotic cell and they are involved in the processing of pro-proteins, pro-hormones, receptors, and pro-viral capsid proteins. Convertases are Ca2+-dependent endopeptidases that recognize and cleave basic sequence Lys-Arg or Arg-Arg present at the C-terminal domain of its protein substrates, and these enzymes are involved in several pathophysiological processes such as: processing of tissue growth factors TGF-² and BMP-4, pro-insulin receptor, glycoprotein gp160 of the HIV virus, the glycoprotein of Ebola virus and anthrax protective antigen. The mammalian PCs family comprises seven members, furin, PACE4, PC4, PC5/6, PC7, PC1 and PC2. The convertase Kex2 is considered the archetype of PCs and was first identified in Saccharomyces cerevisiae. In this project we aimed to study the specificity of convertases Kex2, PC1 and Furin on the hydrolysis of fluorescent type FRET peptide substrates, as well as determine the individual rate constants of each catalytic step of convertases, in other words, determining the binding step of substrate (association constant k1 and dissociation k-1) and catalysis of the substrate (acylation constant k2 and hydrolysis k3) using monitoring techniques of the hydrolysis of the fluorescent substrates, by stopped-flow, and by the effect of temperature on the different rate constants in the enzyme reaction. Also will be studied the effect of allosteric modulators on the activity of convertases, such as: the binding of mono- and divalent metals to enzymes and the effect of interaction with glycosaminoglycans. The effect of allosteric modulation of ligands will also be assessed by the conformational change of the enzymes by intrinsic fluorescence and circular dichroism spectroscopy. Thus, the set of information derived from studies of specificity, thermodynamic analysis, and performance of modulators will allow better understanding of the kinetic behavior of these proteolytic enzymes. (AU)

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Scientific publications (13)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARVALHO, DIEGO B.; DAS NEVES, AMARITH R.; PORTAPILLA, GISELE B.; SOARES, OZILDEIA; SANTOS, LARISSA B. B.; OLIVEIRA, JEFFERSON R. S.; VIANNA, LUAN S.; JUDICE, WAGNER A. S.; CARDOSO, IARA A.; LUCCAS, PEDRO H.; et al. Repurposing of 5-nitrofuran-3,5-disubstituted isoxazoles: A thriving scaffold to antitrypanosomal agents. ARCHIV DER PHARMAZIE, v. N/A, p. 9-pg., . (14/02205-1, 16/25112-4, 21/01503-2)
DA CUNHA, GISLAINE A.; DE SOUZA, RONAN F. F.; DE FARIAS, RENAN L.; MOREIRA, MARIETE B.; SILVA, DEBORA E. S.; ZANETTI, RENAN D.; GARCIA, DANIEL M.; SPINDOLA, DANIEL G.; MICHELIN, LUIS F. G.; BINCOLETTO, CLAUDIA; et al. Cyclopalladated compounds containing 2,6-lutidine: Synthesis, spectral and biological studies. Journal of Inorganic Biochemistry, v. 203, . (12/15486-3, 14/02205-1, 09/54011-8, 16/25112-4)
ANTUNES, ALYNE ALEXANDRINO; PASSOS JESUS, LARISSA DE OLIVEIRA; MANFREDI, MARCELLA ARAUJO; DE SOUZA, ALINE APARECIDA; MARCONDES MACHADO, MAURICIO FERREIRA; MORAES E SILVA, PAMELA; ICIMOTO, MARCELO YUDI; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; DE SOUZA JUDICE, WAGNER ALVES. Thermodynamic analysis of Kex2 activity: The acylation and deacylation steps are potassium- and substrate-dependent. Biophysical Chemistry, v. 235, p. 29-39, . (14/02205-1, 15/11190-0, 16/25112-4)
DE MOURA, THALES REGGIANI; ZANETTI, RENAN DIEGO; SILVA, DEBORA EDUARDA SOARES; DE FARIAS, RENAN LIRA; MAURO, ANTONIO EDUARDO; PEREIRA, JOSE CLAYSTON MELO; DE SOUZA, ALINE APARECIDA; DA SILVA SIQUEIRA, FABIO; DE SOUZA JUDICE, WAGNER ALVES; LIMA, MAURO ALMEIDA; et al. Palladium(ii) complexes bearing 1-iminothiolate-3,5-dimethylpyrazoles: synthesis, cytotoxicity, DNA binding and enzymatic inhibition studies. NEW JOURNAL OF CHEMISTRY, v. 44, n. 45, p. 19891-19901, . (15/12098-0, 16/25112-4, 14/02205-1, 09/54011-8, 19/11242-1, 16/17711-5)
COSTA, CLAUDIA A.; LOPES, RAYSSA M.; FERRAZ, LETICIA S.; ESTEVES, GABRIELA N. N.; DI IORIO, JULIANA F.; SOUZA, ALINE A.; DE OLIVEIRA, ISADORA M.; MANARIN, FLAVIA; JUDICE, WAGNER A. S.; STEFANI, HELIO A.; et al. Cytotoxicity of 4-substituted quinoline derivatives: Anticancer and antileishmanial potential. Bioorganic & Medicinal Chemistry, v. 28, n. 11, . (16/04289-3, 18/25747-5, 16/25112-4, 17/24821-4, 14/02205-1)
ROSA, LETICIA B.; GALUPPO, CAROLINA; LIMA, ROCHANNA L. A.; FONTES, JOSIELLE, V; SIQUEIRA, FABIO S.; JUDICE, WAGNER A. S.; ABBEHAUSEN, CAMILLA; MIGUEL, DANILO C.. Antileishmanial activity and insights into the mechanisms of action of symmetric Au(I) benzyl and aryl-N-heterocyclic carbenes. Journal of Inorganic Biochemistry, v. 229, p. 9-pg., . (14/02205-1, 19/01678-7, 16/25112-4, 18/21120-8, 19/16904-2, 14/21129-4)
JESUS, LARISSA DE OLIVEIRA PASSOS; DE SOUZA, ALINE APARECIDA; TORQUATO, HERON FERNANDES VIEIRA; GONTIJO, VANESSA SILVA; DE FREITAS, ROSSIMIRIAN PEREIRA; GESTEIRA, TARSIS FERREIRA; COULSON-THOMAS, VIVIEN JANE; TORQUATO, RICARDO JOSE SOARES; TANAKA, APARECIDA SADAE; PAREDES-GAMERO, EDGAR JULIAN; et al. In Vitro Study of Cytotoxic Mechanisms of Alkylphospholipids and Alkyltriazoles in Acute Lymphoblastic Leukemia Models. Molecules, v. 27, n. 23, p. 23-pg., . (21/01503-2, 16/25112-4, 14/02205-1)
PAPP, LUIZ ANTONIO; CARDINALI-REZENDE, JULIANA; JUDICE, WAGNER ALVES DE SOUZA; SANCHES, MARILIA BIXILIA; ARAUJO, WELINGTON LUIZ. Total phosphorus contents currently found in the raw wastewater-Problems and technical solutions for its removal in full-scale wastewater treatment plants. RESOURCES CONSERVATION AND RECYCLING, v. 196, p. 10-pg., . (21/01503-2, 16/25112-4, 14/02205-1, 16/24290-6, 14/08061-1, 17/12510-4)
DE SOUZA, ALINE APARECIDA; DE ANDRADE, DEBORA MARTINS; SIQUEIRA, FABIO DA SILVA; DI IORIO, JULIANA FORTES; VELOSO, MARCIA PARANHO; COELHO, CAMILA DE MORAIS; JUNIOR, CLAUDIO VIEGAS; GONTIJO, VANESSA SILVA; DOS SANTOS, MARCELO HENRIQUE; ZOREL MENEGHETTI, MARIA CECILIA; et al. Semysinthetic biflavonoid Morelloflavone-7,4 `,7 `',3 `'',4 `''-penta-O-butanoyl is a more potent inhibitor of Proprotein Convertases Subtilisin/Kexin PC1/3 than Kex2 and Furin. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1865, n. 12, . (14/02205-1)
PAPP, LUIZ ANTONIO; RODRIGUES, FLAVIO APARECIDO; DE SOUZA JUDICE, WAGNER ALVES; ARAUJO, WELINGTON LUIZ. Onsite Wastewater Treatment Upgrade for Water Reuse in Cooling Towers and Toilets. WATER, v. 14, n. 10, p. 14-pg., . (17/12510-4, 21/01503-2, 14/02205-1, 16/25112-4)
MANFREDI, MARCELLA ARAUJO; ANTUNES, ALYNE ALEXANDRINO; PASSOS JESUS, LARISSA DE OLIVEIRA; JULIANO, MARIA APARECIDA; JULIANO, LUIZ; DE SOUZA JUDICE, WAGNER ALVES. Specificity characterization of the alpha-mating factor hormone by Kex2 protease. Biochimie, v. 131, p. 149-158, . (14/02205-1)
PAPP, LUIZ ANTONIO; CARDINALI-REZENDE, JULIANA; DE SOUZA JUDICE, WAGNER ALVES; SANCHEZ, MARILIA BIXILIA; ARAUJO, WELINGTON LUIZ. Low biological phosphorus removal from effluents treated by slow sand filters. Applied Microbiology and Biotechnology, v. 106, n. 17, p. 13-pg., . (21/01503-2, 17/12510-4, 16/25112-4, 14/02205-1)
LOURENCO, ESTELA MARIANA GUIMARAES; DI IORIO, JULIANA FORTES; DA SILVA, FERNANDA; FIALHO, FELIPE LEONARDO BLEY; MONTEIRO, MELQUISEDEQUE MATEUS; BEATRIZ, ADILSON; PERDOMO, RENATA TRENTIN; BARBOSA, EUZEBIO GUIMARAES; OSES, JEAN PIERRE; DE ARRUDA, CARLA CARDOZO PINTO; et al. Flavonoid Derivatives as New Potent Inhibitors of Cysteine Proteases: An Important Step toward the Design of New Compounds for the Treatment of Leishmaniasis. MICROORGANISMS, v. 11, n. 1, p. 23-pg., . (21/01503-2, 16/25112-4, 14/02205-1)

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