Study of micro-exon genes (MEGs) of the human parasite Schistosoma mansoni and of the interaction of their protein products with human cells

• Grant number: 14/09361-9
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2014
• End date: November 30, 2015
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Ricardo de Marco
• Grantee: Ricardo de Marco
• Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
• Associated researchers: Ana Paula Ulian de Araujo

Abstract

This project has the objective to study Micro-Exon Genes and their protein products that are found in the host-parasite interface. We will perform experiments to understand the interaction of PCM (Proteins coded by MEGs) with human proteins and cells. Previous results obtained by our group demonstrated that PCMs are involved in erythrocyte hemolysis, as well as the interaction with proteins from human lymphocytes. In addition, we will continue bioinformatic studies trying to understand the evolution of MEG genic structure. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LI, XIAO-HONG; DEMARCO, RICARDO; NEVES, LEANDRO X.; JAMES, SALLY R.; NEWLING, KATHERINE; ASHTON, PETER D.; CAO, JIAN-PING; WILSON, R. ALAN; CASTRO-BORGES, WILLIAM. Microexon gene transcriptional profiles and evolution provide insights into blood processing by the Schistosoma japonicum esophagus. PLoS Neglected Tropical Diseases, v. 12, n. 2, FEB 2018. (14/09361-9)

ORCIA, DEBORA; ZERAIK, ANA ELIZA; LOPES, JOSE L. S.; MACEDO, JOCI N. A.; DOS SANTOS, CLARISSA ROMANO; OLIVEIRA, KATIA C.; ANDERSON, LETICIA; WALLACE, B. A.; VERJOVSKI-ALMEIDA, SERGIO; ARAUJO, ANA P. U.; et al. Interaction of an esophageal MEG protein from schistosomes with a human S100 protein involved in inflammatory response. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1861, n. 1, p. 8-pg., 2017-01-01. (12/07288-7, 13/20715-4, 14/09361-9, 12/09186-7, 10/20290-5)

WILSON, R. ALAN; LI, XIAO HONG; MACDONALD, SANDY; NEVES, LEANDRO XAVIER; VITORIANO-SOUZA, JULIANA; LEITE, LUCIANA C. C.; FARIAS, LEONARDO P.; JAMES, SALLY; ASHTON, PETER D.; DEMARCO, RICARDO; et al. The Schistosome Esophagus Is a `Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing. PLoS Neglected Tropical Diseases, v. 9, n. 12, DEC 2015. (12/23124-4, 14/09361-9, 12/18095-5)

ORCIA, DEBORA; ZERAIK, ANA ELIZA; LOPES, JOSE L. S.; MACEDO, JOCI N. A.; DOS SANTOS, CLARISSA ROMANO; OLIVEIRA, KATIA C.; ANDERSON, LETICIA; WALLACE, B. A.; VERJOVSKI-ALMEIDA, SERGIO; ARAUJO, ANA P. U.; et al. Interaction of an esophageal MEG protein from schistosomes with a human S100 protein involved in inflammatory response. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1861, n. 1, A, p. 3490-3497, JAN 2017. (12/07288-7, 13/20715-4, 14/09361-9, 10/20290-5, 12/09186-7)

WILSON, R. ALAN; LI, XIAO HONG; MACDONALD, SANDY; NEVES, LEANDRO XAVIER; VITORIANO-SOUZA, JULIANA; LEITE, LUCIANA C. C.; FARIAS, LEONARDO P.; JAMES, SALLY; ASHTON, PETER D.; DEMARCO, RICARDO; et al. The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing. PLoS Neglected Tropical Diseases, v. 9, n. 12, p. 25-pg., 2015-12-01. (12/23124-4, 12/18095-5, 14/09361-9)

FELIZATTI, ANA P.; ZERAIK, ANA E.; BASSO, LUIS G. M.; KUMAGAI, PATRICIA S.; LOPES, JOSE L. S.; WALLACE, B. A.; ARAUJO, ANA P. U.; DEMARCO, RICARDO. Interactions of amphipathic alpha-helical MEG proteins from Schistosoma mansoni with membranes. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1862, n. 3, MAR 1 2020. (14/09361-9, 14/00206-0, 18/19546-7, 15/50347-2)