Proteomic analysis of Biomphalaria glabrata infected with Schistosoma mansoni and Angiostrongylus cantonensis

• Grant number: 16/07137-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2016
• End date: October 31, 2018
• Field of knowledge: Biological Sciences - Parasitology - Entomology and Malacology of Parasites and Vectors
• Principal Investigator: Silmara Marques Allegretti
• Grantee: Silmara Marques Allegretti
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Ana Júlia Pinto Fonseca Sieuve Afonso ; Emanuel Carrilho ; Fernanda Janku Cabral ; Tiago Manuel Fernandes Mendes

Abstract

In Brazil, there are three species of Biomphalaria that are responsible for the transmission of Schistosoma mansoni, Biomphalaria glabrata, Biomphalaria tenagophila and Biomphalaria straminea. Species of Biomphalaria are capable of maintaining their self-integrity, mainly through their internal defense system composed of hemocytes and soluble proteins. Biomphalaria are capable of recognizing parasite molecules activating their phagocytic cells that may lead to their encasement and destruction. Concomitant parasite infections interfere with the internal defense system of the snail, therefore co-infection of different helminthes infections may be a useful tool in the snail defense mechanisms against parasites. Angiostrongylus cantonensis is the main cause of human eosinophilic meningitis. This parasite is capable of completing their larval development in B. glabrata. The infection by A. cantonensis in B. glabrata leads to an increment on hemocytes numbers and encasement of parasite larvae. Our general goal is the characterization of immune and proteomic changes in Biomphalaria glabrata throughout the infection cycle of Schistosoma mansoni and Angiostrongylus cantonensis, in both simple infection and in co-infection, in order to find molecular markers for helminthic specificity to snail infection. Understanding the parasite - intermediary host interactions has a high biomedical importance, since without snails, there would be no infection, therefore, it is important to understand what factors allow the development of the parasite in the intermediary host. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, ROSIMEIRE N.; CORREA, SHEILA A. P.; VIEIRA, KAREN M.; MENDES, TIAGO; ALLEGRETTI, SILMARA M.; MIGUEL, DANILO C.. In vitro schistosomicidal activity of tamoxifen and its effectiveness in a murine model of schistosomiasis at a single dose. Parasitology Research, v. 118, n. 5, p. 1625-1631, MAY 2019. (16/07137-0, 14/21129-4)

RIBEIRO SOTELO MONTANHAUR, ALLAN DANIEL; LIMA, ESTELA DE OLIVEIRA; DELAFIORI, JEANY; ESTEVES, CIBELE ZANARDI; RIBEIRO PRADO, CESAR CORAT; ALLEGRETTI, SILMARA MARQUES; UETA, MARLENE TIDUKO; LEVY, CARLOS EMILIO; CATHARINO, RODRIGO RAMOS. Metabolic alterations in Strongyloidiasis stool samples unveil potential biomarkers of infection. Acta Tropica, v. 227, p. 6-pg., 2022-03-01. (19/05718-3, 16/07137-0)

MENDES, TIAGO M. F.; CARRILHO, EMANUEL; GALINARO, CARLOS A.; CABRAL, FERNANDA J.; ALLEGRETTI, SILMARA M.. Biomphalaria glabrata infected with Angiostrongylus cantonensis: Proteomic changes in the snail host. Acta Tropica, v. 212, DEC 2020. (17/07364-9, 16/07137-0, 09/54040-8)

FERNANDES MENDES, TIAGO MANUEL; CARRILHO, EMANUEL; FONSECA SIEUVE AFONSO, ANA JULIA PINTO; GALINARO, CARLOS ALEXANDRE; CABRAL, FERNANDA JANKU; ALLEGRETTI, SILMARA MARQUES. Proteomic, metabolic and immunological changes in Biomphalaria glabrata infected with Schistosoma mansoni. International Journal for Parasitology, v. 49, n. 13-14, p. 1049-1060, DEC 2019. (09/54040-8, 16/07137-0, 17/07364-9)