The effect of bradykinin on osteoclastogenesis in vitro and LPS-induced bone resorption in vivo

• Grant number: 14/05283-3
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: November 01, 2014
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Pedro Paulo Chaves de Souza
• Grantee: Pedro Paulo Chaves de Souza
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Carlos Alberto de Souza Costa ; João Antonio Chaves de Souza ; Petra Henning ; Ulf Holger Lerner
• Associated research grant(s): 16/50019-8 - Molecular mechanisms involved in osteoimmunomodulatory properties of biomaterials, AP.R SPRINT
• Associated scholarship(s): 15/00410-0 - The role of IL-4 on RANKL induced by lipoproteins in osteoblasts, BP.IC

Abstract

Besides the classical action of the kallikrein-kinin system (KKS) in the modulation of the vascular tonus, this system is also involved in different pathologies, some of them being inflammatory in nature. Inflammatory processes in the vicinity of the skeleton generally leads to bone loss, and in vitro models demonstrate that kinins may participate of inflammatory bone resorption by activating the B1 and B2 kinin receptors. In contrast with these findings, some in vivo studies demonstrate an inhibitory role of the KKS on bone resorption and osteoclast differentiation. Since several drugs having the KKS as target are available in the market, the knowledge about the mechanisms underlying the role of kinins on bone resorption is very important to predict side effects and develop new drugs. Therefore, the aim of the present project is to analyze the role of kinins on LPS-induced bone resorption in vivo and the role of kinins on osteoclastogenesis in vitro. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JOÃO ANTONIO CHAVES DE SOUZA; FERNANDO AUGUSTO CINTRA MAGALHÃES; GUILHERME JOSE PIMENTEL LOPES DE OLIVEIRA; RAFAEL SCAF DE MOLON; JOSÉ ANTONIO ZUANON; PEDRO PAULO CHAVES DE SOUZA. Pam2CSK4 (TLR2 agonist) induces periodontal destruction in mice. Brazilian Oral Research, v. 34, 2020-02-10. (15/21697-5, 14/05283-3)

SOUZA, PEDRO P. C.; LERNER, ULF H.. Finding a Toll on the Route: The Fate of Osteoclast Progenitors After Toll-Like Receptor Activation. FRONTIERS IN IMMUNOLOGY, v. 10, JUL 17 2019. (14/05283-3)

PERSSON, EMMA; SOUZA, PEDRO P. C.; FLORIANO-MARCELINO, THAIS; CONAWAY, HOWARD HERSCHEL; HENNING, PETRA; LERNER, ULF H.. Activation of Shc1 Allows Oncostatin M to Induce RANKL and Osteoclast Formation More Effectively Than Leukemia Inhibitory Factor. FRONTIERS IN IMMUNOLOGY, v. 10, MAY 28 2019. (14/05283-3, 15/00410-0)

DE SOUZA, PEDRO P. C.; HENNING, PETRA; LERNER, ULF H.. Stimulation of Osteoclast Formation by Oncostatin M and the Role of WNT16 as a Negative Feedback Regulator. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 6, p. 22-pg., 2022-03-01. (14/05283-3, 15/00410-0)

MOVERARE-SKRTIC, SOFIA; NILSSON, KARIN H.; HENNING, PETRA; FUNCK-BRENTANO, THOMAS; NETHANDER, MARIA; RIVADENEIRA, FERNANDO; NUNES, GLAUCIA COLETTO; KOSKELA, ANTTI; TUUKKANEN, JUHA; TUCKERMANN, JAN; et al. Osteoblast-derived NOTUM reduces cortical bone mass in mice and the NOTUM locus is associated with bone mineral density in humans. FASEB JOURNAL, v. 33, n. 10, p. 11163-11179, OCT 2019. (14/05283-3)

CHAVES DE SOUZA, JOAO ANTONIO; CINTRA MAGALHAES, FERNANDO AUGUSTO; PIMENTEL LOPES DE OLIVEIRA, GUILHERME JOSE; DE MOLON, RAFAEL SCAF; ZUANON, JOSE ANTONIO; CHAVES DE SOUZA, PEDRO PAULO. Pam2CSK4 (TLR2 agonist) induces periodontal destruction in mice. BRAZILIAN ORAL RESEARCH, v. 34, p. 10-pg., 2020-01-01. (15/21697-5, 14/05283-3)

LEGUIZAMON, N. DA PONTE; DE MOLO, R. S.; COLETTO-NUNES, G.; NOGUEIRA, A. V. B.; ROCHA, V, S.; NEO-JUSTINO, D. M.; SOARES-COSTA, A.; CERRI, P. S.; LERNER, U. H.; SOUZA, P. P. C.; et al. hytocystatin CsinCPI-2 Reduces Osteoclastogenesis and Alveolar Bone Los. JOURNAL OF DENTAL RESEARCH, v. 101, n. 2, JUL 2021. (14/05283-3, 18/10727-9, 18/10728-5, 16/09876-4, 15/21697-5)

HENNING, PETRA; MOVERARE-SKRTIC, SOFIA; WESTERLUND, ANNA; CHAVES DE SOUZA, PEDRO PAULO; FLORIANO-MARCELINO, THAIS; NILSSON, KARIN H.; EL SHAHAWY, MAHA; OHLSSON, CLAES; LERNER, ULF H.. WNT16 is Robustly Increased by Oncostatin M in Mouse Calvarial Osteoblasts and Acts as a Negative Feedback Regulator of Osteoclast Formation Induced by Oncostatin M. JOURNAL OF INFLAMMATION RESEARCH, v. 14, p. 4723-4741, 2021. (15/00410-0, 14/05283-3)

SOUZA, PEDRO P. C.; LUNDBERG, PERNILLA; LUNDGREN, INGER; MAGALHAES, FERNANDO A. C.; COSTA-NETO, CLAUDIO M.; LERNER, ULF H.. Activation of Toll-like receptor 2 induces B1 and B2 kinin receptors in human gingival fibroblasts and in mouse gingiva. SCIENTIFIC REPORTS, v. 9, FEB 27 2019. (14/05283-3)

TEIXEIRA, JORGE FELIPE LIMA; DE SOUZA, JOAO ANTONIO CHAVES; MAGALHAES, FERNANDO AUGUSTO CINTRA; DE OLIVEIRA, GUILHERME JOSE PIMENTEL LOPES; DE SANTIS, JOSE BERNARDO; COSTA, CARLOS ALBERTO DE SOUZA; DE SOUZA, PEDRO PAULO CHAVES. Laser-Modified Ti Surface Improves Paracrine Osteogenesis by Modulating the Expression of DKK1 in Osteoblasts. JOURNAL OF FUNCTIONAL BIOMATERIALS, v. 14, n. 4, p. 12-pg., 2023-04-01. (14/05283-3, 17/22739-9)