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Quantification and characterization of Salmonella spp. isolates in sewage sludge with potential usage in agriculture

Grant number: 14/50731-4
Support type:Regular Research Grants - Publications - Scientific article
Duration: December 01, 2014 - May 31, 2015
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Maria Tereza Pepe Razzolini
Grantee:Maria Tereza Pepe Razzolini
Home Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil


This study aims to scrutinize Salmonella spp. and its serotypes in sewage sludge samples from wastewater treatment plants, and assesses the presence of virulence genes and antibiotics resistant to the profile. Samples (n=54) were collected and analyzed in accordance with the EPA Method 1682/2006. For positive serological reaction, 40 strains were selected for PCR analyses and detection of spvC, invA and sseL virulence genes, plasmid presence and resistance to antibiotics. Salmonella spp. was detected in 38.9% of the samples collected «0.006473 to 12.19 MPN/gTS). The most prevalent serotype was Salmonella infantis. All Salmonella spp. (n=35) presented at least one of the three virulence genes mentioned above and 40% harbored plasmids. Salmonella Typhimurium strains were isolated harboring at least one of the following virulence genes: spvC, invA ar sseL. Four Salmonella spp. isolates were resistant to tetracycline; three were resistant to trimethoprim-sulfamethoxazole, and one isolate was resistant to ciprofloxacin. Two Salmonella spp. strains presented multi resistance to antimicrobial agents. (AU)