Selection and characterization probiotic Lactobacillus spp. in vitro with Salmonella Heidelberg inhibition potential isolated from healthy turkeys

• Grant number: 15/00383-2
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: March 31, 2016
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Adriano Sakai Okamoto
• Grantee: Adriano Sakai Okamoto
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Raphael Lucio Andreatti Filho

Abstract

The health of birds is closely related to how they are created, with the primary concern of intestinal microbiota in the full development of their productive potential. The lactobacilli bacteria which are part of the natural flora of poultry and are commonly used as probiotics. For it must have many characteristics that make them potential candidates, such as resistance to gastric pH and bile salts, potential hydrophobicity and multiplication, hydrogen peroxide production and antagonism to pathogens, key criteria for the selection of bacterial samples the application in animals, so this way act protecting the gastrointestinal tract against the action of microorganisms such as Salmonella spp., pathogen that can harm the health of the animal. Currently Salmonella Heidelberg is a zoonotic disease because it can affect the poultry breeding and cause untold damage the health of birds and human. This paper aims to complete the functional characterization and in vitro probiotic of Lactobacillus spp. from the cecum of healthy adult turkeys the isolation and identification of biochemical assays and polymerase chain reaction technique Amplified Ribosomal DNA Restriction Analysis, assessment of resistance of bacteria against artificial gastric juice and the bile salt tolerance, the hydrophobicity of the cell surface , the potential for multiplication, hydrogen peroxide production, antibiotic susceptibility testing and antagonism against Salmonella Heidelberg, thus evaluating the functionality of bacteria of the genus Lactobacillus spp. against a pathogen. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALTARUGIO, R.; VELLANO, I. H. B.; MORAES, A. C. I.; MILBRADT, E. L.; ANDREATTI FILHO, R. L.; GUIMARAES-OKAMOTO, P. T. C.; PADOVANI, C. R.; OKAMOTO, A. S.. In vitro probiotic selection and characterization of Lactobacillus spp. isolated from healthy domesticated Turkeys. JOURNAL OF APPLIED POULTRY RESEARCH, v. 27, n. 1, p. 81-91, MAR 2018. (15/00383-2)