Evaluation of organic acids and probiotics as an alternative to antimicrobial additiveson performance parameters, intestinal microflora and challenge with Salmonella Enteritidis

• Grant number: 10/20655-3
• Support Opportunities: Regular Research Grants
• Duration: March 01, 2011 - February 28, 2013
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Raphael Lucio Andreatti Filho
• Grantee: Raphael Lucio Andreatti Filho
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Ana Angelita Sampaio Baptista ; Elisane Lenita Milbradt ; João Pessoa Araújo Junior ; Ticiana Silva Rocha

Abstract

In order to evaluate alternatives to antimicrobials will be conducted two experiments, in Experiment I will evaluate the effectiveness of different products used as growth promoters, it will be used 420 commercial turkeys female were divided into four groups with five repetitions: diet basal, diet supplemented with zinc bacitracin; diet with probiotic and diet with organic acids during the growing period. The variables are evaluated: performance, intestinal morphology, measurement of pH in the crop and cecum, quantification of volatil fatty acids and quantification of the bacteria Lactobacillus spp., Clostridium spp., Bacteriodes spp. and the Enterobacteriaceae family. In Experiment II will aim to assess the effect of administration of organic acids, probiotics and antibiotics on the population of Lactobacillus spp. and enterobacterias, including elimination of Salmonella Enteritidis (SE) (challenge) in the crop and cecum of turkeys fasted pre-slaughter. It will also be evaluated external contamination of birds after challenge with SE in pre slaughter. This will be used 147 birds divided into seven groups of three repetitions: no challenge with SE, with SE challenge; organic acids in drinking water and SE; probiotic in drinking water and SE; probiotic in the feed and SE; organic acids in the feed and SE, zinc bacitracin in the feed and SE. Before slaughter, all birds will undergo 12 hours of fasting and water fasting for six hours. After the birds will be culled by cervical dislocation after collecting samples for microbiological analysis. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

EL MILBRADT; AS OKAMOTO; CR PADOVANI; VB FASCINA; TM SILVA; R ALTARÚGIO; A HATAKA; EMS SCHMIDT; RL ANDREATTI FILHO. Use of Organic Acids and A Competitive Exclusion Product as Growth Promoter and Salmonella Enteritidis Control in Commercial Turkeys. Brazilian Journal of Poultry Science, v. 19, n. 4, p. 551-558, 2017-12-00. (10/20655-3, 11/07752-2)