Microbiomics and proteomics in the communication of lactic acid bacteria, enhancing the action against Salmonella Heidelberg in chickens by bacteria from the intestinal microbiota

• Grant number: 21/10630-8
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2022
• End date: January 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Adriano Sakai Okamoto
• Grantee: Adriano Sakai Okamoto
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Josias Rodrigues ; Lucilene Delazari dos Santos ; Raphael Lucio Andreatti Filho

Abstract

According to the Brazilian Association of Animal Protein, in 2019, Brazil was ranked 1st in chicken meat exports, increasing concerns about health, further favoring this evolution. However, the spread of pathogens remains a challenge, especially from Salmonella spp., the bacteria that causes salmonellosis, a disease that can affect birds, being considered one of the most common and important zoonoses. Thus, to prevent colonization of the intestinal mucosa by enteropathogens, products with the function of modulating the intestinal microbiota of the bird, such as probiotics, mainly composed of lactic acid bacteria (BALs) were developed. In addition to the direct antagonism of salmonella, BALs exert a bacterial communication mechanism, which urges the intestinal microbiota to collectively inhibit the pathogen. Upon reaching a minimum population density, this behavior is triggered, leading to the recognition and secretion of inducing peptides. The aim of this study will be to evaluate the potentiation of the inhibition of Salmonella Heidelberg by samples of lactic acid bacteria, in vitro and in vivo, modulated by inducers of bacterial communication, analyzing the composition of the inducer by proteomics and evaluation of the cecal microbiota of the bird by microbiomic analysis before and after bacterial communication. (AU)