Functionalized metal oxides thin films for the study of protein adsorption

• Grant number: 14/20471-0
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2014
• End date: November 30, 2016
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Paulo Noronha Lisboa Filho
• Grantee: Paulo Noronha Lisboa Filho
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
• Associated researchers: Erika Soares Bronze-Uhle ; Jose Humberto Dias da Silva ; Luís Augusto Sousa Marques da Rocha

Abstract

This proposal is inserted into a multidisciplinary context, as a part of the new subject of nanobiotechnology. Nanostructured metal oxides (TiO2 and ZnO) have been used as surface modifiers in medical implants, promoting improvements in the biocorrosion effects and increased oxides bioactivity, which indicates promising results in the interaction with living tissues. Despite of recognized biocompatibility of these oxides, there still are many aspects of the mechanisms of adhesion between the proteins and the metal oxides surfaces.Different in vitro and in vivo tests demonstrate that the biocompatibility and biocorrosion properties depend on the composition, thickness, homogeneity and porosity of the oxide. Chemically, the surface of such oxides are mainly terminated by -OH groups which can be readily functionalized. This chemical functionalization or simply a physical change in the material surface can improve the interaction of the oxide with the biological environment.This project proposes to improve understanding of the mechanisms of adsorption of proteins on different structures of metal oxides. The aim of this study is the evaluation the physicochemical and thermodynamic parameters, in order to better analyze the interaction and adsorption of protein to the oxide covered metal surface, since different biomedical applications require different properties of protein -oxide interaction. Different strategies for functionalization and adsorption will be studied and tested in biological systems. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GOMES, ORISSON P.; AZEVEDO NETO, NILTON F.; BRONZE-UHLE, ERIKA S.; TRINO, LUCIANA D.; DOS SANTOS, CASSIO M.; DA SILVA, JOSE H. D.; LISBOA-FILHO, PAULO N.. 3-Mercaptopropionic acid functionalization of titanium dioxide thin films. Materials Chemistry and Physics, v. 223, p. 32-38, FEB 1 2019. (16/22186-7, 13/07296-2, 14/20471-0)

GOMES, O. P.; FELTRAN, G. S.; FERREIRA, M. R.; ALBANO, C. S.; ZAMBUZZI, W. F.; LISBOA-FILHO, P. N.. A novel BSA immobilizing manner on modified titanium surface ameliorates osteoblast performance. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 190, JUN 2020. (16/22186-7, 14/22689-3, 17/15035-5, 13/07296-2, 14/20471-0)

TRINO, LUCIANA D.; BRONZE-UHLE, ERIKA S.; RAMACHANDRAN, AMSAVENI; LISBOA-FILHO, PAULO N.; MATHEW, MATHEW T.; GEORGE, ANNE. Titanium surface bio-functionalization using osteogenic peptides: Surface chemistry, biocompatibility, corrosion and tribocorrosion aspects. JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS, v. 81, p. 26-38, MAY 2018. (14/01713-3, 14/27015-0, 13/07296-2, 14/20471-0)

BRONZE-UHLE, ERIKA S.; DIAS, LEONARDO F. G.; TRINO, LUCIANA D.; MATOS, ADRIANA A.; DE OLIVEIRA, RODRIGO C.; LISBOA-FILHO, PAULO N.. Physicochemical characterization of albumin immobilized on different TiO2 surfaces for use in implant materials. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 564, p. 39-50, MAR 5 2019. (14/01713-3, 14/20471-0, 16/11183-7, 17/15035-5)

BRONZE-UHLE, ERIKA S.; DIAS, LEONARDO F. G.; TRINO, LUCIANA D.; MATOS, ADRIANA A.; DE OLIVEIRA, RODRIGO C.; LISBOA-FILHO, PAULO N.. Physicochemical bisphosphonate immobilization on titanium dioxide thin films surface by UV radiation for bio-application. SURFACE & COATINGS TECHNOLOGY, v. 357, p. 36-47, JAN 15 2019. (14/01713-3, 14/20471-0, 16/11183-7, 17/15035-5)

TRINO, LUCIANA D.; DIAS, LEONARDO F. G.; ALBANO, LUIZ G. S.; BRONZE-UHLE, ERIKA S.; RANGEL, ELIDIANE C.; GRAEFF, CARLOS F. O.; LISBOA-FILHO, PAULO N.. Zinc oxide surface functionalization and related effects on corrosion resistance of titanium implants. CERAMICS INTERNATIONAL, v. 44, n. 4, p. 4000-4008, MAR 2018. (14/01713-3, 13/09963-6, 13/07296-2, 14/20471-0)

TRINO, LUCIANA D.; BRONZE-UHLE, ERIKA S.; GEORGE, ANNE; MATHEW, MATHEW T.; LISBOA-FILHO, PAULO N.. Surface Physicochemical and Structural Analysis of Functionalized Titanium Dioxide Films. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 546, p. 168-178, JUN 5 2018. (14/01713-3, 14/27015-0, 13/07296-2, 14/20471-0)