Comparative genomics of three strains of Pseudomonas putida detected in different environments of Coffea arabica plants and evaluation of the growth promotion potential in coffee

• Grant number: 14/11544-4
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2014
• End date: December 31, 2017
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Jorge Maurício Costa Mondego
• Grantee: Jorge Maurício Costa Mondego
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated researchers: Carlos Augusto Colombo ; Marcelo Brocchi ; Marcelo Falsarella Carazzolle ; Márcio José da Silva

Abstract

Pseudomonas putida is a bacterial species with ability to colonize a wide range of environments. P. putida is known mainly as plant growth promoting bacteria. The production of auxins and siderophores by P. putida lead to the improvement of vegetal development, confer yield increment and give better protection against pathogens. During the annotation of C. arabica ESTs databank, we detected a considerable amount of Pseudomonas sequences, what suggest that Pseudomonads inhabit C. arabica plants. Through the isolation of bacteria from leaves (phylosphere), rizosphere and from the internal environment (endophytics) of C. arabica roots, five P. putida isolates were detected. The production of auxins and siderophores and resistance against caffeine and water stress were tested for the five isolates. Interestingly, a correlation between physiological data and environment of each isolate was detected. These project aims to verify the genomic differences among the isolates, correlating them to preliminary physiological data, which will be repeated and extended during the project. For that, three isolates, each one isolated from a different niche, will have its genome sequenced and compared. Such approach, that have been named as 'pan-genomics', aims to understand why organisms from the same species, sharing the same host, exhibit distinct ecological and physiological behaviors. In addition, these isolates will be inoculated in C. arabica seeds and data from plant growth will be recorded and correlated with data from bacterial physiology and genomics. This knowledge will allow a better exploitation of the potential of such Pseudomonas as coffee growth promoters. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE SOUSA, LEANDRO PIO; PEREIRA CIPRIANO, MATHEUS APARECIDO; DA SILVA, MARCIO JOSE; ALVES PATRICIO, FLAVIA RODRIGUES; FREITAS, SUELI DOS SANTOS; CARAZZOLLE, MARCELO FALSARELLA; COSTA MONDEGO, JORGE MAURICIO. Functional genomics analysis of a phyllospheric Pseudomonas spp with potential for biological control against coffee rust. BMC Microbiology, v. 22, n. 1, p. 12-pg., 2022-09-22. (14/11544-4)

DE SOUSA, LEANDRO PIO; CIPRIANO, MATHEUS APARECIDO PEREIRA; FREITAS, SUELI DOS SANTOS; CARAZZOLLE, MARCELO FALSARELLA; DA SILVA, MARCIO JOSE; MONDEGO, JORGE MAURICIO COSTA. Genomic and physiological evaluation of two root associated Pseudomonas from Coffea arabica. MICROBIOLOGICAL RESEARCH, v. 263, p. 11-pg., 2022-07-27. (14/11544-4)

DE SOUSA, LEANDRO PIO; DA SILVA, MARCIO JOSE; COSTA MONDEGO, JORGE MAURICIO. Leaf-associated bacterial microbiota of coffee and its correlation with manganese and calcium levels on leaves. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 2, p. 11-pg., 2018-04-01. (14/11544-4)

DE SOUSA, LEANDRO PIO; DA SILVA, MARCIO JOSE; COSTA MONDEGO, JORGE MAURICIO. Leaf-associated bacterial microbiota of coffee and its correlation with manganese and calcium levels on leaves. GENETICS AND MOLECULAR BIOLOGY, v. 41, n. 2, p. 455-465, APR-JUN 2018. (14/11544-4)