| Grant number: | 14/22594-2 |
| Support Opportunities: | Regular Research Grants |
| Start date: | March 01, 2015 |
| End date: | February 28, 2017 |
| Field of knowledge: | Agronomical Sciences - Agronomy - Plant Health |
| Principal Investigator: | Marcelo Eiras |
| Grantee: | Marcelo Eiras |
| Host Institution: | Instituto Biológico (IB). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil |
| Associated researchers: | Addolorata Colariccio ; Alexandre Levi Rodrigues Chaves ; Alice Kazuko Inoue Nagata ; Elliot Watanabe Kitajima ; Jorge Alberto Marques Rezende ; Kátia Regiane Brunelli-Braga ; Renata Faier Calegario ; Ricardo Harakava |
| Associated research grant(s): | 15/50076-9 - Characterisation of Potyviruses infecting vegetable crops in Brazil and the stability of resistance identified in Europe to South American Potyviruses, AP.R SPRINT |
Abstract
Turnip mosaic virus (TuMV, Potyvirus) is one of the most important viruses that cause damage in several economical crops, especially Brassicaceae such as kale, cabbage, cauliflower, broccoli (Brassica oleraceae), turnip (B. rapa), mustard (B. juncea), canola (B. napus) and radish (Raphanus sativus). This virus is distributed worldwide, has the broadest known host range and, consequently, present high genetic diversity. TuMV isolates have been classified in different strains and pathotypes based on their ability to infect certain plant species and cultivars. Isolates of TuMV were characterized in several countries in Europe, Asia, North America, Africa and Oceania. However, there is a lack of knowledge about TuMV genetic diversity in South America, including Brazil, where sporadic observations of its occurrence were reported. The goals of this project are: (i) to evaluate the incidence of TuMV in Brazil by surveys of cultivated Brassicaceae as kale, cabbage, cauliflower, broccoli, turnip, canola, radish, and wild spontaneous Brassicaceae species [B. rapa (wild mustard), Coronopus didymus, Lepidium virginicum, Raphanus raphanistrum (wild radish) and Sinapis arvensis (white mustard)], potential natural reservoirs of TuMV; (ii) to characterize TuMV isolates by mechanical inoculation on indicator and differential plants (strains of B. napus) in order to evaluate whether TuMV Brazilian isolates are capable of overcoming brassica resistance genes; (iii) molecular characterization of TuMV isolates by sequencing the genomic portion corresponding to the coat protein (CP), and the full genomic sequence of, at least, three Brazilian TuMV isolates; (iv) to evaluate the association of wild plants, potential natural reservoirs of TuMV in the cultivated areas of Brassica spp., to the presence and incidence of TuMV; (v) to evaluate efficiency of transmission of different aphid species, which can act as vectors of TuMV in the field. (AU)
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