Killer toxin production by Sporobolomyces koalae (ACBL-77) and Saccharomyces cerevisiae (ACB-K1) as mode of action against postharvest pathogens

• Grant number: 14/25067-3
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: September 30, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Katia Cristina Kupper
• Grantee: Katia Cristina Kupper
• Host Institution: Instituto Agronômico (IAC). Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Campinas , SP, Brazil
• Associated researchers: João Martins Pizauro Junior ; Marco Aurélio Takita ; Marcos Antonio Machado

Abstract

In order to understand as the killer toxins produced by Sporobolomyces koalae (ACBL-77) or Saccharomyces cerevisiae (ACB-K1) could kill sensitive yeast strain (S. cerevisiae NCYC 1006) including the pathogens Penicillium digitatum and Geotrichum citri- aurantii, the extracellular killer toxins in the supernatants of cell culture of yeast isolates will be purified and their antagonistic activities tested. The killer toxin purified from S. koalae will be characterized and their molecular mass will be estimated according to the data from SDS-PAGE. The optimal conditions for action of the purified killer toxin against the pathogens, as well as the identification of the gene encoding it will be evaluated. The identification of the type of killer toxin of S. cerevisiae (ACB-K1) will be accomplished by PCR amplification using specific primers of proteins known and produced by S. cerevisiae (K1, K2, and K28), and subsequently sequencing will be performed. If the amplification it is not possible, the characterization of killer toxin produced by ACB-K1 will be held following the same steps described for isolated ACBL-77. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MOURA, VANESSA S.; POLLETTINI, FLAVIA L.; FERRAZ, LURIANY P.; MAZZI, MAURICIO V.; KUPPER, KATIA C.. Purification of a killer toxin from Aureobasidium pullulans for the biocontrol of phytopathogens. JOURNAL OF BASIC MICROBIOLOGY, v. 61, n. 2, DEC 2020. (14/25067-3)