Deciphering the molecular interaction between Puccinia psidii x Eucalyptus grandis through "omics" approaches

• Grant number: 14/16804-4
• Support Opportunities: Research Grants - Young Investigators Grants
• Start date: June 01, 2015
• End date: May 31, 2019
• Field of knowledge: Biological Sciences - Genetics - Molecular Genetics and Genetics of Microorganisms
• Principal Investigator: Maria Carolina Quecine Verdi
• Grantee: Maria Carolina Quecine Verdi
• Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
• Associated researchers: Carlos Alberto Labate ; Claudia Barros Monteiro Vitorello ; Gabriel Rodrigues Alves Margarido ; João Lúcio de Azevedo
• Associated scholarship(s): 17/11026-1 - Bacterial biosynthesis of indole acetic acid l-tryptophan dependent and influence on the modulation of vegetal growth, BP.MS; 16/16868-8 - Modulation of the interaction among growth promoting bacteria and corn by the quorum sensing system, BP.MS

Abstract

Certainly the microorganisms are the most versatile form of life. They collaborate with many natural processes and interact with a huge diversity of other organisms. Among the complex microbe-host interactions, the plant pathogens have been better studied mainly due the vast global economics losses and environmental impacts caused by them. Currently, the high-throughput sequencing approaches as genomics, transcriptomics and proteomics have contributed with a better understood of the complex pathogen-plant molecular interactions. These studies are more relevant when applied to understanding pathosystems with significant economic importance and with incipient molecular information as instance eucalyptus rust caused by biotrophic fungus Puccinia psidii. This phytopathogen is originated from South America. As a cosmopolite pathogen, the P. psidii affects many species of Eucalyptus around the world with different degrees of severity, E. grandis being one of the most susceptible hosts. Based on the system biology, the aiming of the present project is the overview and the integration of genomic, transcriptomic and proteomic of P. psidii during its morphogenesis and under Eucalyptus spp. infection process. The obtained data will collaborate with a better understanding of the fungal biology and its pathogenesis process allowing the development of a novel database of genes, transcripts and proteins of P. psidii which will support other research groups as well as will provide some clues about the unknown lifestyle of this bitrophic pathogen. The generated data will also support the application of system biology concepts on pathogen-plant interaction studies by the integration of genomic, transcriptomic and proteomic data to deciphering under a holistic view the molecular plant-microbe interaction that is few explored on Brazil. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERRAREZI, JESSICA A.; MCTAGGART, ALISTAIR R.; TOBIAS, PERI A.; HAYASHIBARA, CAROLINA A. A.; DEGNAN, REBECCA M.; SHUEY, LOUISE S.; FRANCESCHINI, LIVIA M.; LOPES, MARIANA S.; QUECINE, MARIA C.. Austropuccinia psidii uses tetrapolar mating and produces meiotic spores in older infections on Eucalyptus grandis. Fungal Genetics and Biology, v. 160, p. 8-pg., 2022-05-01. (14/16804-4, 19/25720-2)

BINI, ANDRESSA PERES; QUECINE, MARIA CAROLINA; DA SILVA, THALITA MORAES; SILVA, LUCIANA DUQUE; LABATE, CARLOS ALBERTO. Development of a quantitative real-time PCR assay using SYBR Green for early detection and quantification of Austropuccinia psidii in Eucalyptus grandis. European Journal of Plant Pathology, v. 150, n. 3, p. 735-746, MAR 2018. (08/50361-1, 13/07596-6, 14/16804-4)

DOS SANTOS, ISANELI BATISTA; LOPES, MARIANA DA SILVA; BINI, ANDRESSA PERES; PROHMANN TSCHOEKE, BRUNO AUGUSTO; WRUCK VERSSANI, BRUNA APARECIDA; FIGUEREDO, EVERTHON FERNANDES; CATALDI, THAIS REGIANI; RODRIGUES MARQUES, JOAO PAULO; SILVA, LUCIANA DUQUE; LABATE, CARLOS ALBERTO; et al. The Eucalyptus Cuticular Waxes Contribute in Preformed Defense Against Austropuccinia psidii. FRONTIERS IN PLANT SCIENCE, v. 9, JAN 9 2019. (14/16804-4, 12/25315-1, 17/11026-1)

DE ALMEIDA, JAQUELINE RAQUEL; RIANO PACHON, DIEGO MAURICIO; FRANCESCHINI, LIVIA MARIA; DOS SANTOS, ISANELI BATISTA; FERRAREZI, JESSICA APARECIDA; MAIA DE ANDRADE, PEDRO AVELINO; MONTEIRO-VITORELLO, CLAUDIA BARROS; LABATE, CARLOS ALBERTO; QUECINE, MARIA CAROLINA. Revealing the high variability on nonconserved core and mobile elements of Austropuccinia psidii and other rust mitochondrial genomes. PLoS One, v. 16, n. 3, MAR 11 2021. (14/16804-4, 16/16868-8)

HAYASHIBARA, CAROLINA ALESSANDRA DE ALMEIDA; LOPES, MARIANA DA SILVA; TOBIAS, PERI A.; SANTOS, ISANELI BATISTA DOS; FIGUEREDO, EVERTHON FERNANDES; FERRAREZI, JESSICA APARECIDA; MARQUES, JOAO PAULO RODRIGUES; MARCON, JOELMA; PARK, ROBERT F.; TEIXEIRA, PAULO JOSE PEREIRA LIMA; et al. In Planta Study Localizes an Effector Candidate from Austropuccinia psidii Strain MF-1 to the Nucleus and Demonstrates In Vitro Cuticular Wax-Dependent Differential Expression. JOURNAL OF FUNGI, v. 9, n. 8, p. 16-pg., 2023-08-01. (14/16804-4, 19/25720-2)