Lipid oxidation in membrane and cell biophysics: from functional nanosensors to impact on amyloid formation. Application of advanced fluorescence, X-ray scattering and microscopy techniques

• Grant number: 14/20107-7
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2016
• End date: September 30, 2020
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Agreement: Fundação para a Ciência e a Tecnologia (FCT)
• Principal Investigator: Rosangela Itri
• Grantee: Rosangela Itri
• Principal researcher abroad: Manuel José Estevez Prieto
• Institution abroad: Associação do Instituto Superior Técnico para a Investigação e Desenvolvimento (IST-ID), Portugal
• Host Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Ana Isabel Abrantes Coutinho ; Andreza Barbosa Gomide ; Fabio Monteiro Fernandes ; Leandro Ramos Souza Barbosa ; Mário Nuno de Matos Sequeira Berberan e Santos ; Mauricio da Silva Baptista

Abstract

Lipid oxidation is a degenerative process that disturbs the structure and function of cells with pathologic consequences as Parkinson's and Alzheimer's diseases, atherosclerosis, diabetes, aging and carcinogenesis. In this project, we joint complementary expertises to explore how oxidized lipids affect the biophysical properties of lipid bilayers in terms of membrane integrity, permeability, as well as formation of lipid rafts. In the following, we aim to unveil the role of oxidized lipids in promoting amyloidogenic aggregates formation on membrane surface. Further, by means of oxygen sensor, we investigate the consequences of singlet oxygen production as well as its preferential location within the cell after a photosensition process. The experimental tools will comprise SAXS, optical microscopy and advanced fluorescence techniques. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TSUBONE, TAYANA MAZIN; JUNQUEIRA, HELENA COUTO; BAPTISTA, MAURICIO S.; ITRI, ROSANGELA. Contrasting roles of oxidized lipids in modulating membrane microdomains. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, v. 1861, n. 3, p. 660-669, MAR 1 2019. (11/00963-8, 14/20107-7, 12/50680-5, 16/23071-9)

TSUBONE, TAYANA MAZIN; BAPTISTA, MAURICIO S.; ITRI, ROSANGELA. Understanding membrane remodelling initiated by photosensitized lipid oxidation. Biophysical Chemistry, v. 254, NOV 2019. (14/20107-7, 16/23071-9)

SCANAVACHI, GUSTAVO; COUTINHO, ANA; FEDOROV, ALEXANDER ANDREEVICH; PRIETO, MANUEL; MELO, ANA M.; ITRI, ROSANGELA. Lipid Hydroperoxide Compromises the Membrane Structure Organization and Softens Bending Rigidity. Langmuir, v. 37, n. 33, p. 9952-9963, AUG 24 2021. (14/20107-7)