Characterization and study of oxidases enzymes from filamentous fungi

• Grant number: 14/24107-1
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: October 01, 2015
• End date: September 30, 2017
• Field of knowledge: Biological Sciences - Biochemistry - Biochemistry of Microorganisms
• Principal Investigator: Felipe Santiago Chambergo Alcalde
• Grantee: Felipe Santiago Chambergo Alcalde
• Host Institution: Escola de Artes, Ciências e Humanidades (EACH). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• City of the host institution: São Paulo

Abstract

During the enzymatic hydrolysis of lignocellulosic biomass mixtures of enzymes are used for soluble fermentable sugars. The main enzymes in the mixture are: cellobiohydrolases, endoglucanases, b-glucosidase, a-xylosidases, a-arabinofuranosidases, pectinases, feruloilesterases, oxidoreductase and other accessory enzymes. Recent research has demonstrated the importance and participation of auxiliary or accessory enzymes as oxidoreductase enzymes (MCO and PMO) on degradation of lignocellulose. This proposal aims to study and characterization of oxidoreductases enzymes produced by filamentous fungi during their growth in medium containing sugar cane bagasse. The identification of oxidoreductases enzyme mixtures with potential use in the hydrolysis of lignocellulose allow efficient enzymatic treatment, and obtaining higher level of sugars from biomass. (AU)

Scientific publications

(9)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

DE OLIVEIRA, GABRIEL STEPHANI; ADRIANI, PATRICIA PEREIRA; BORGES, FLAVIA GARCIA; LOPES, ADRIANA RIOS; CAMPANA, PATRICIA T.; CHAMBERGO, FELIPE S.. Epoxide hydrolase of Trichoderma reesei: Biochemical properties and conformational characterization. International Journal of Biological Macromolecules, v. 89, p. 569-574, AUG 2016. (14/24107-1, 15/03329-9)

WILSON, CAROLINA; DE OLIVEIRA, GABRIEL S.; ADRIANI, PATRICIA P.; CHAMBERGO, FELIPE S.; DIAS, MARCIO V. B.. Structure of a soluble epoxide hydrolase identified in Trichoderma reesei. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1865, n. 8, p. 1039-1045, AUG 2017. (15/09188-8, 10/15971-3, 14/24107-1, 15/03329-9)

DE OLIVEIRA, GABRIEL S.; ADRIANI, PATRICIA P.; WU, HAO; MORISSEAU, CHRISTOPHE; HAMMOCK, BRUCE D.; CHAMBERGO, FELIPE S.. Substrate and inhibitor selectivity, and biological activity of an epoxide hydrolase from Trichoderma reesei. MOLECULAR BIOLOGY REPORTS, v. 46, n. 1, p. 371-379, FEB 2019. (16/12859-4, 15/03329-9, 14/24107-1, 17/25705-8)

COSTA, RICARDO BEZERRA; CAMPANA, PATRICIA TARGON; CHAMBERGO, FELIPE SANTIAGO; NAPOLEAO, THIAGO HENRIQUE; GUEDES PAIVA, PATRICIA MARIA; VIEIRA PEREIRA, HUGO JUAREZ; VILELA OLIVA, MARIA LUIZA; GOMES, FRANCIS SOARES. Purification and characterization of a lectin with refolding ability from Genipa americana bark. International Journal of Biological Macromolecules, v. 119, p. 517-523, NOV 2018. (14/24107-1)

DEOLIVEIRA, GABRIEL STEPHANI; ADRIANI, PATRICIA PEREIRA; BORGES, FLAVIA GARCIA; LOPES, ADRIANA RIOS; CAMPANA, PATRICIA T.; CHAMBERGO, FELIPE S.. Data set of optimal parameters for colorimetric red assay of epoxide hydrolase activity. DATA IN BRIEF, v. 8, p. 436-440, SEP 2016. (14/24107-1, 15/03329-9)

CHAMBERGO, FELIPE S.; VALENCIA, ESTELA Y.. Fungal biodiversity to biotechnology. Applied Microbiology and Biotechnology, v. 100, n. 6, p. 2567-2577, MAR 2016. (12/50153-5, 14/24107-1)

DE OLIVEIRA, GABRIEL S.; ADRIANI, PATRICIA P.; RIBEIRO, JOAO AUGUSTO; MORISSEAU, CHRISTOPHE; HAMMOCK, BRUCE D.; DIAS, MARCIO VINICIUS B.; CHAMBERGO, FELIPE S.. The molecular structure of an epoxide hydrolase from Trichoderma reesei in complex with urea or amide-based inhibitors. International Journal of Biological Macromolecules, v. 129, p. 653-658, MAY 15 2019. (13/15906-5, 16/12859-4, 15/03329-9, 15/09188-8, 18/00351-1, 14/24107-1, 17/25705-8)

DE MELO, VANDIERLY SAMPAIO; GOMES, BRISA MOREIRA; GAZIRI, TARCILLO; TAMAROZZI, ELVIRA REGINA; CHAMBERGO, FELIPE SANTIAGO. Biochemical characterization and molecular dynamics study of a thermostable endo-1,5-α-arabinanase. Protein Expression and Purification, v. 231, p. 12-pg., 2025-04-21. (17/25705-8, 14/24107-1)

ADRIANI, PATRICIA P.; DE PAIVA, FERNANDA C. R.; DE OLIVEIRA, GABRIEL S.; LEITE, AMANDA C.; SANCHES, ADRIANA S.; LOPES, ADRIANA RIOS; DIAS, MARCIO V. B.; CHAMBERGO, FELIPE S.. Structural and functional characterization of the glutathione peroxidase-like thioredoxin peroxidase from the fungus Trichoderma reesei. International Journal of Biological Macromolecules, v. 167, p. 93-100, JAN 15 2021. (18/00351-1, 17/25705-8, 19/05227-0, 14/24107-1)

Filed patent(s) as a result of this research project