Dental pulp stem-cell viability, proliferation and osteogenic potential in PCL/Poli(rotaxane) membranes

• Grant number: 15/15055-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2015
• End date: October 31, 2017
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Maria Cristina Zindel Deboni
• Grantee: Maria Cristina Zindel Deboni
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Carla Renata Sipert ; Marcia Martins Marques

Abstract

Despite autogenous graft is still considered the gold standard in bone reconstruction, because of osteogenesis, osteoinduction and osteoconduction properties, it has some disadvantages. The majority complications are related to the donor site. Therefore, a series of new bone substitutes have been developed, and groundbreaking search has been a constant in bioengineering. Currently, besides bone substitutes, absorbable membranes have been occupied an important role in guided bone regeneration (GBR). These membranes were made of a great variety of biomaterials, non-absorbable and absorbable. Among absorbable materials, electro spinning polymers composites can be highlighted because of its low manufacturing cost and its high potential biological applications. The Materials and Engineering Department of UNICAMP developed a membrane composed by polymeric blends of PCL(policaprolactone) and polyrotaxane that according to the literature have important mechanical properties and high biocompatibility. Furthermore it has slow in vivo degradation rate, thus providing an adequate support until regeneration is completed. Low resorption rate is recommended for long-term applications, as slow release of bioactive molecules or substances. The purpose of this project is an in vitro evaluation of PCL/polyrotaxane membrane biological effect and its possible osteogenic potential. To answer our purpose, dental pulp stem cells will be seeded on PCL/ polyrotaxane membrane samples for cellular cytotoxicity, proliferation and adhesion. In addition alkaline phosphatase activity, mineral nodules formation and genic expression will be analyzed. If the results show that this polymeric composite could present osteogenic potential it will be matter of a new proposal to test a functionalized membrane with stem cells as a future alternative for critical bone defects. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COSTA SALLES, TAIS HELENA; SENDYK, DANIEL ISAAC; DE OLIVEIRA, NATACHA KALLINE; MACHADO, DAISY; LANCELLOTTI, MARCELO; ZINDEL DEBONI, MARIA CRISTINA; KIANG, CHANG TIEN; D'AVILA, MARCOS AKIRA. In vitro and in vivo evaluation of electrospun membranes of poly (epsilon-caprolactone) and poly (rotaxane). Materials Science & Engineering C-Materials for Biological Applications, v. 77, p. 912-919, AUG 1 2017. (15/15055-0)

OLIVEIRA, N. K.; SALLES, T. H. C.; PEDRONI, A. C.; MIGUITA, L.; D'AVILA, M. A.; MARQUES, M. M.; DEBONI, M. C. Z.. Osteogenic potential of human dental pulp stem cells cultured onto poly-epsilon-caprolactone/poly (rotaxane) scaffolds. Dental Materials, v. 35, n. 12, p. 1740-1749, DEC 2019. (15/15055-0)

DE OLIVEIRA, NATACHA KALLINE; FERRAZ, EMANUELA PRADO; PERILLO ROSIN, FLAVIA CRISTINA; CORREA, LUCIANA; ZINDEL DEBONI, MARIA CRISTINA. Poly-epsilon-caprolactone/poly(rotaxane) seeded with human dental pulp stem cells or osteoblasts promotes angiogenesis: a chorioallantoic membrane assay. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v. N/A, p. 16-pg., 2022-06-20. (15/15055-0)