Effect of supplementation with brazil nut (Bertholettia excelsa H.B.K) on inflammation, oxidative stress and the expression of selenoprotein p and microRNAs in obese women

• Grant number: 15/02906-2
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2015
• End date: January 31, 2018
• Field of knowledge: Health Sciences - Nutrition - Nutrition Biochemistry
• Principal Investigator: Silvia Maria Franciscato Cozzolino
• Grantee: Silvia Maria Franciscato Cozzolino
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Marcelo Macedo Rogero

Abstract

Inflammation and oxidative stress are processes that occur simultaneously in obesity and contribute to an increased synthesis of inflammatory mediators and reactive oxygen and nitrogen species. The mineral selenium (Se), whose main food source is the Brazil nut (Bertholettia excelsa H.B.K), has antioxidant and anti-inflammatory properties. Considering the importance of inflammation in obesity and the potential anti-inflammatory role of selenium through selenoproteins, the influence of this micronutrient in this context is of great importance. In addition, the study of possible molecular mechanisms, such as microRNAs (miRNA), can provide more specific answers about the role of selenium in these pathways. This study aims to evaluate the effect the Brazil nut consumption as a source of Se on inflammation, oxidative stress and the expression of selenoprotein P and microRNAs in obese women. This will be a prospective longitudinal study. We will select 50 obese women, aged between 18 and 50 years, which will receive about 200 µg /Se a day, through the ingestion of Brazil-Brazil, for 60 days. We will assess before and after the intervention, the biochemical parameters related to the nutritional status of Se, oxidative stress, lipid profile, inflammatory markers and gene expression of selenoprotein P and microRNAs. Dietary intake of macronutrients and Se will be evaluated through three 24-hour recalls, performed before and after intervention. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

QUINTANILHA, BRUNA J.; REIS, BRUNA Z.; SILVA DUARTE, GRAZIELA B.; COZZOLINO, SILVIA M. F.; ROGERO, MARCELO M.. Nutrimiromics: Role of microRNAs and Nutrition in Modulating Inflammation and Chronic Diseases. NUTRIENTS, v. 9, n. 11, NOV 2017. (15/02906-2)

SILVA DUARTE, GRAZIELA BIUDE; REIS, BRUNA ZAVARIZE; ROGERO, MARCELO MACEDO; BARBOSA JR, FERNANDO; CERCATO, CINTIA; FRANCISCATO COZZOLINO, SILVIA MARIA. Plasma Concentration of Essential and Toxic Trace Elements After Brazil Nut Intake: Results from a Randomized Controlled Trial. BIOLOGICAL TRACE ELEMENT RESEARCH, v. N/A, p. 6-pg., 2022-04-29. (15/02906-2)

SILVA DUARTE, GRAZIELA BIUDE; REIS, BRUNA ZAVARIZE; ROGERO, MARCELO MACEDO; VARGAS-MENDEZ, ERNESTO; BARBOSA JUNIOR, FERNANDO; CERCATO, CINTIA; FRANCISCATO COZZOLINO, SILVIA MARIA. Consumption of Brazil nuts with high selenium levels increased inflammation biomarkers in obese women: A randomized controlled trial. NUTRITION, v. 63-64, p. 162-168, JUL-AUG 2019. (15/02906-2)

REIS, BRUNA ZAVARIZE; SILVA DUARTE, GRAZIELA BIUDE; VARGAS-MENDEZ, ERNESTO; PINTO FERREIRA, LUDMILA RODRIGUES; BARBOSA JR, FERNANDO; CERCATO, CINTIA; ROGERO, MARCELO MACEDO; FRANCISCATO COZZOLINO, SILVIA MARIA. Brazil nut intake increases circulating miR-454-3p and miR-584-5p in obese women. Nutrition Research, v. 67, p. 40-52, JUL 2019. (15/02906-2)