Study of the molecular diversity of strains of Staphylococcus aureus isolated from bovine mastitis

• Grant number: 15/10332-6
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2015
• End date: October 31, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcos Bryan Heinemann
• Grantee: Marcos Bryan Heinemann
• Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Adriana Cortez ; Alessandro Conrado de Oliveira Silveira ; Alessandro de Sá Guimaraes ; Carla Christine Lange ; Carla Gasparotto Chande Vasconcelos ; Fernando Nogueira de Souza ; John Anthony McCulloch ; Maria Aparecida Vasconcelos Paiva e Brito

Abstract

Mastitis is considered the disease of greatest impact in the global dairy industry and in Brazil, production losses between 12 and 15% are estimated, which represents about three billion liters of milk / year. However, despite adoption of the current management practices to minimize the occurrence of mastitis, results are not satisfactory for the control of mastitis by Staphylococcus aureus, the major causative agent of bovine mastitis. Besides veterinary importance, there are also implications for human health due to the potential for zoonotic spread of S. aureus by unpasteurized dairy products, and due to the extensive use of antibiotics in the treatment and control of disease. Molecular epidemiology studies are important in the understanding of the genetic diversity among causative agents of mastitis in a region or country, the distribution of pathogen groups within and between herds and their phylogenetic relationship. Typing by sequencing single locus (SPA typing) and multilocus (MLST) has been successfully used to investigate the population structure of pathogenic bacteria, especially S. aureus. Both methods are suitable for epidemiological studies since they provide information that can be compared between different laboratories. Comparison of the profile (sequence type ST) among S. aureus from different sources (animals and properties) is useful in the understanding of the role of these hosts as reservoirs for pathogenic strains and of the epidemiological aspects involved in the transmission of this pathogen. This project aims to understand the genetic diversity of S. aureus strains isolated from bovine mastitis, identify possible new ST profiles, and to know the distribution of ST in space and time. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FREU, GUSTAVO; TOMAZI, TIAGO; FILHO, ANTONIO F. S.; HEINEMANN, MARCOS B.; DOS SANTOS, MARCOS, V. Antimicrobial Resistance and Molecular Characterization of Staphylococcus aureus Recovered from Cows with Clinical Mastitis in Dairy Herds from Southeastern Brazil. ANTIBIOTICS-BASEL, v. 11, n. 4, p. 11-pg., 2022-04-01. (13/22286-3, 14/17411-6, 15/10332-6)

TOMAZI, T.; COURA, F. M.; GONCALVES, J. L.; HEINEMANN, M. B.; SANTOS, M. V.. Antimicrobial susceptibility patterns of Escherichia coli phylogenetic groups isolated from bovine clinical mastitis. JOURNAL OF DAIRY SCIENCE, v. 101, n. 10, p. 9406-9418, OCT 2018. (13/22286-3, 14/17411-6, 15/10332-6)

TOMAZI, TIAGO; FREU, GUSTAVO; ALVES, BRUNA GOMES; DE SOUZA FILHO, ANTONIO FRANCISCO; HEINEMANN, MARCOS BRYAN; DOS SANTOS, MARCOS VEIGA. Genotyping and antimicrobial resistance of Streptococcus uberis isolated from bovine clinical mastitis. PLoS One, v. 14, n. 10, OCT 22 2019. (15/10332-6, 13/22286-3, 14/17411-6)

DORNELES, ELAINE M. S.; FONSECA, MARIANA D. A. M.; ABREU, JULIANA A. P.; LAGE, ANDREY P.; BRITO, MARIA A. V. P.; PEREIRA, CARINE R.; BRANDAO, HUMBERTO M.; GUIMARAES, ALESSANDRO S.; HEINEMANN, MARCOS B.. Genetic diversity and antimicrobial resistance in Staphylococcus aureus and coagulase-negative Staphylococcus isolates from bovine mastitis in Minas Gerais, Brazil. MICROBIOLOGYOPEN, v. 8, n. 5, MAY 2019. (15/10332-6)

SANTOS, RENATA P.; SOUZA, FERNANDO N.; OLIVEIRA, ANA CLAUDIA D.; DE SOUZA FILHO, ANTONIO F.; AIZAWA, JULIANA; MORENO, LUISA Z.; CUNHA, ADRIANO F.; CORTEZ, ADRIANA; DELLA LIBERA, ALICE M. M. P.; HEINEMANN, MARCOS B.; et al. Molecular Typing and Antimicrobial Susceptibility Profile of Staphylococcus aureus Isolates Recovered from Bovine Mastitis and Nasal Samples. ANIMALS, v. 10, n. 11, NOV 2020. (14/23189-4, 15/10332-6)

AIZAWA, JULIANA; SOUZA-FILHO, ANTONIO F.; GUIMARAES, ALESSANDRO S.; VASCONCELOS, CARLA G. C.; BRITO, MARIA APARECIDA V. P.; SELLERA, FABIO P.; CORTEZ, ADRIANA; HEINEMANN, MARCOS B.. Retrospective multicenter study reveals absence of MRSA-associated bovine mastitis in Brazil (1994 to 2016). JOURNAL OF INFECTION IN DEVELOPING COUNTRIES, v. 13, n. 6, p. 581-583, JUN 2019. (15/10332-6)

FERRONATTO, J. A.; SOUZA, F. N.; DELLA LIBERA, A. M. M. P.; DE VLIEGHER, S.; DE VISSCHER, A.; PIEPERS, S.; BLAGITZ, M. G.; HEINEMANN, M. B.. Inhibition of the growth of major mastitis-causing pathogens by non-aureus Staphylococcus isolates using the cross-streaking method. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 71, n. 5, p. 1745-1749, SEP-OCT 2019. (14/23189-4, 15/10332-6)

TOMAZI, TIAGO; DE SOUZA FILHO, ANTONIO FRANCISCO; HEINEMANN, MARCOS BRYAN; DOS SANTOS, MARCOS VEIGA. Molecular characterization and antimicrobial susceptibility pattern of Streptococcus agalactiae isolated from clinical mastitis in dairy cattle. PLoS One, v. 13, n. 6, JUN 21 2018. (13/22286-3, 14/17411-6, 15/10332-6)

PEREZ, VERONICA K. C.; CUSTODIO, DIRCEIA A. C.; SILVA, EDUARDA M. M.; DE OLIVEIRA, JULIA; GUIMARAES, ALESSANDRO S.; BRITO, MARIA A. V. P.; SOUZA-FILHO, ANTONIO F.; HEINEMANN, MARCOS B.; LAGE, ANDREY P.; DORNELES, ELAINE M. S.. Virulence factors and antimicrobial resistance inStaphylococcus aureusisolated from bovine mastitis in Brazil. Brazilian Journal of Microbiology, v. 51, n. 4, AUG 2020. (15/10332-6)