Molecular detection and characterization of agents belonging to Anaplasmataceae e Bartonellaceae family in wild animals and ectoparasites in Brazil

• Grant number: 15/14896-1
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2015
• End date: November 30, 2017
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
• Principal Investigator: Marcos Rogério André
• Grantee: Marcos Rogério André
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

The assessment of the role of wild mammals in cycles transmission of bacteria belonging to Anaplasmataceae e Bartonellaceae family has contributed to the determination of important points to the epidemiology of diseases caused by these agents in several regions of the planet. In this context, new genotypes of Ehrlichia spp. and Anaplasma spp. have been detected in wild animals in Brazil. Additionally, Bartonella spp. has been incriminated as reemergent pathogens in Brazil and other countries in the world. The zoonotic agents belonging to Anaplasmataceae e Bartonellaceae provide a perfect opportunity to reinforce the One Health concept (i.e., the need to integrate animal and human health, and environmental factors) while coordinating research priorities designed to enhance diagnosis, treatment, and prevention of Bartonella spp. infections. A better understanding of the interactions of vectors, animals, people, and the environment will allow for improvements in epidemiologic and medical knowledge of the myriad disease manifestations and clinical presentations collectively known as ehrlichiosis, anaplasmosis and bartonellosis. The present work aims to investigate the occurrence and assess the phylogenetic inference of Anaplasmataceae e Bartonellaceae agents in wild animals Brazil, using molecular techniques. The global project involves four subprojects: 1) wild animals (carnivores, rodents and marsupials) and domestic (dogs) and their respective ectoparasites in Pantanal sul-matogrossense; 2) wild and synantropic rodents from several Brazilian biomes; 3) bats sampled in localities in the states of Pará and Paraná; 4) ectoparasites (Streblidae flies and Macronyssidae e Spinturnicidae mites) collected from bats in Rio de Janeiro. The present work aims to contribute to the diagnosis and molecular characterization of Anaplasmataceae and Bartonellaceae agents in our country. (AU)

Scientific publications

(26)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

INALDA ANGÉLICA DE SOUZA RAMOS; HEITOR MIRAGLIA HERRERA; NATÁLIA SERRA MENDES; SIMONE DE JESUS FERNANDES; JOÃO BOSCO VILELA CAMPOS; JOÃO VITOR ALMEIDA ALVES; GABRIEL CARVALHO DE MACEDO; ROSANGELA ZACARIAS MACHADO; MARCOS ROGÉRIO ANDRÉ. Filogeografia de genótipos msp4 de Anaplasma marginale em gado de corte no Pantanal brasileiro. Revista Brasileira de Parasitologia Veterinária, v. 28, n. 3, p. 451-457, 2019-08-01. (15/14896-1)

COSTA DE OLIVEIRA BRAGA, MARIA DO SOCORRO; PEREIRA, JOSE GOMES; FERNANDES, SIMONE DE JESUS; LOPES MARQUES, INGRID CAROLINNE; DE JESUS, RENATA PASSOS; FERREIRA, GLEYCIANNY SANTOS; XAVIER, DANIELE ROSA; BENEVENUTE, JYAN LUCAS; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Molecular detection of Anaplasmataceae agents in Dasyprocta azarae in northeastern Brazil. Revista Brasileira de Parasitologia Veterinária, v. 27, n. 1, p. 99-105, JAN-MAR 2018. (15/14896-1)

BENEVENUTE, JYAN LUCAS; DUMLER, JOHN STEPHEN; OGRZEWALSKA, MARIA; RODRIGUES ROQUE, ANDRE LUIZ; CALIFRE MELLO, VICTORIA VALENTE; MARQUES DE SOUSA, KEYLA CARSTENS; GONCALVES, LUIZ RICARDO; D'ANDREA, PAULO SERGIO; DE SAMPAIO LEMOS, ELBA REGINA; MACHADO, ROSANGELA ZACARIAS; et al. Assessment of a quantitative 5 ` nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil. TICKS AND TICK-BORNE DISEASES, v. 8, n. 4, p. 646-656, 2017. (15/14896-1)

MENDES, NATALIA SERRA; DE SOUZA RAMOS, INALDA ANGELICA; HERRERA, HEITOR MIRAGLIA; VILELA CAMPOS, JOAO BOSCO; DE ALMEIDA ALVES, JOAO VICTOR; DE MACEDO, GABRIEL CARVALHO; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Genetic diversity of Babesia bovis in beef cattle in a large wetland in Brazil. Parasitology Research, v. 118, n. 7, p. 2027-2040, JUL 2019. (15/14896-1)

GONCALVES, LUIZ RICARDO; DE MENDONCA FAVACHO, ALEXSANDRA RODRIGUES; RODRIGUES ROQUE, ANDRE LUIZ; MENDES, NATALIA SERRA; FIDELIS JUNIOR, OTAVIO LUIZ; BENEVENUTE, JYAN LUCAS; HERRERA, HEITOR MIRAGLIA; D'ANDREA, PAULO SERGIO; SAMPAIO DE LEMOS, ELBA REGINA; MACHADO, ROSANGELA ZACARIAS; et al. Association of Bartonella Species with Wild and Synanthropic Rodents in Different Brazilian Biomes. Applied and Environmental Microbiology, v. 82, n. 24, p. 7154-7164, DEC 2016. (15/14896-1)

MARQUES DE SOUSA, KEYLA CARSTENS; FERNANDES, MARINA PUGNAGHI; HERRERA, HEITOR MIRAGLIA; BENEVENUTE, JYAN LUCAS; SANTOS, FILIPE MARTINS; ROCHA, FABIANA LOPES; GOMES BARRETO, WANESSA TEIXEIRA; MACEDO, GABRIEL CARVALHO; CAMPOS, JOAO BOSCO; MARTINS, THIAGO FERNANDES; et al. Molecular detection of Hepatozoon spp. in domestic dogs and wild mammals in southern Pantanal, Brazil with implications in the transmission route. Veterinary Parasitology, v. 237, p. 37-46, APR 15 2017. (15/14896-1, 13/13186-5, 16/10676-0)

MARQUES DE SOUSA, KEYLA CARSTENS; FERNANDES, MARINA PUGNAGHI; HERRERA, HEITOR MIRAGLIA; FRESCHI, CARLA ROBERTA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Diversity of piroplasmids among wild and domestic mammals and ectoparasites in Pantanal wetland, Brazil. TICKS AND TICK-BORNE DISEASES, v. 9, n. 2, p. 245-253, FEB 2018. (15/14896-1, 13/13186-5, 16/10676-0)

DO AMARAL, RENAN BRESSIANINI; LOURENCO, ELIZABETE CAPTIVO; FAMADAS, KATIE MARIA; GARCIA, AMANDA BARBOSA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Molecular detection of Bartonella spp. and Rickettsia spp. in bat ectoparasites in Brazil. PLoS One, v. 13, n. 6, JUN 5 2018. (15/14896-1, 16/12574-0)

DE SOUZA RAMOS, INALDA ANGELICA; HERRERA, HEITOR MIRAGLIA; FERNANDES, SIMONE DE JESUS; DO AMARAL, RENAN BRESSIANINI; DE SOUZA ZANATTO, DIEGO CARLOS; VIERA DA SILVA, THIAGO MERIGHI; HORTA, BRUNA LE SENECHAL; VILELA CAMPOS, JOAO BOSCO; ALMEIDA ALVES, JOAO VITOR; DE MACEDO, GABRIEL CARVALHO; et al. Genetic diversity of Anaplasma marginale in beef cattle in the Brazilian Pantanal. TICKS AND TICK-BORNE DISEASES, v. 10, n. 4, p. 805-814, JUN 2019. (15/14896-1)

FERNANDES, SIMONE DE JESUS; MATOS, CARLOS ANTONIO; FRESCHI, CARLA ROBERTA; DE SOUZA RAMOS, INALDA ANGELICA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Diversity of Anaplasma species in cattle in Mozambique. TICKS AND TICK-BORNE DISEASES, v. 10, n. 3, p. 651-664, APR 2019. (15/14896-1)

DE SOUSA, K. C. M.; CALCHI, A. C.; HERRERA, H. M.; DUMLER, J. S.; BARROS-BATTESTI, D. M.; MACHADO, R. Z.; ANDRE, M. R.. Anaplasmataceae agents among wild mammals and ectoparasites in Brazil. EPIDEMIOLOGY AND INFECTION, v. 145, n. 16, p. 3424-3437, DEC 2017. (15/14896-1, 13/13186-5, 15/22387-0)

WERTHER, KARIN; LUZZI, MAYARA DE CASSIA; GONCALVES, LUIZ RICARDO; DE OLIVEIRA, JULIANA PAULA; FERREIRA ALVES JUNIOR, JOSE ROBERTO; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Arthropod-borne agents in wild Orinoco geese (Neochen jubata) in Brazil. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, v. 55, p. 30-41, DEC 2017. (15/14896-1, 11/11230-1, 13/15688-8)

MARQUES DE SOUSA, KEYLA CARSTENS; HERRERA, HEITOR MIRAGLIA; SECATO, CAROLINE TOSTES; OLIVEIRA, ANDRE DO VALE; SANTOS, FILIPE MARTINS; ROCHA, FABIANA LOPES; GOMES BARRETO, WANESSA TEIXEIRA; MACEDO, GABRIEL CARVALHO; ESTRELA DE ANDRADE PINTO, PEDRO CORDEIRO; MACHADO, ROSANGELA ZACARIAS; et al. Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland. Acta Tropica, v. 171, p. 172-181, JUL 2017. (16/01216-5, 15/14896-1, 13/13186-5)

BENEVENUTE, JYAN LUCAS; DUMLER, JOHN STEPHEN; OGRZEWALSKA, MARIA; RODRIGUES ROQUE, ANDRE LUIZ; CALIFRE MELLO, VICTORIA VALENTE; MARQUES DE SOUSA, KEYLA CARSTENS; GONCALVES, LUIZ RICARDO; D'ANDREA, PAULO SERGIO; DE SAMPAIO LEMOS, ELBA REGINA; MACHADO, ROSANGELA ZACARIAS; et al. Assessment of a quantitative 5 ' nuclease real-time polymerase chain reaction using groEL gene for Ehrlichia and Anaplasma species in rodents in Brazil. TICKS AND TICK-BORNE DISEASES, v. 8, n. 4, p. 11-pg., 2017-01-01. (15/14896-1)

BOUER, ANDREA; ANDRE, MARCOS ROGERIO; GONCALVES, LUIZ RICARDO; LUZZI, MAYARA DE CASSIA; DE OLIVEIRA, JULIANA PAULA; RODRIGUES, ADRIANA CARLOS; VARANI, ALESSANDRO DE MELO; OLIVEIRA DE MIRANDA, VITOR FERNANDES; PERLES, LIVIA; WERTHER, KARIN; et al. Hepatozoon caimani in Caiman crocodilus yacare (Crocodylia, Alligatoridae) from North Pantanal, Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 26, n. 3, p. 7-pg., 2017-07-01. (15/14896-1)

DE SOUZA RAMOS, INALDA ANGELICA; HERRERA, HEITOR MIRAGLIA; MENDES, NATALIA SERRA; FERNANDES, SIMONE DE JESUS; VILELA CAMPOS, JOAO BOSCO; ALMEIDA ALVES, JOAO VITOR; DE MACEDO, GABRIEL CARVALHO; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Phylogeography of msp4 genotypes of Anaplasma marginale in beef cattle from the Brazilian Pantanal. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 28, n. 3, p. 7-pg., 2019-07-01. (15/14896-1)

MARIA DO SOCORRO COSTA DE OLIVEIRA BRAGA; JOSÉ GOMES PEREIRA; SIMONE DE JESUS FERNANDES; INGRID CAROLINNE LOPES MARQUES; RENATA PASSOS DE JESUS; GLEYCIANNY SANTOS FERREIRA; DANIELE ROSA XAVIER; JYAN LUCAS BENEVENUTE; ROSANGELA ZACARIAS MACHADO; MARCOS ROGÉRIO ANDRÉ. Detecção molecular de agentes Anaplasmataceae em Dasyprocta azarae no nordeste do Brasil. Revista Brasileira de Parasitologia Veterinária, v. 27, n. 1, p. 98-104, 2018-02-19. (15/14896-1)

MARIANA APARECIDA TOLEDO; ALEXANDRO IRIS LEITE; LUIZ RICARDO GONÇALVES; KEYLA CARSTENS MARQUES DE SOUSA; RENAN BRESSIANINI DO AMARAL; GLAUCENYRA CECÍLIA PINHEIRO DA SILVA; ROSANGELA ZACARIAS MACHADO; MARCOS ROGÉRIO ANDRÉ. Alta ocorrência de infecção por Mycoplasma suis em suínos de criações não tecnificadas de Mossoró, estado do Rio Grande do Norte, Nordeste brasileiro. Revista Brasileira de Parasitologia Veterinária, v. 25, n. 4, p. 414-417, 2016-12-08. (15/14896-1, 14/00931-7)

BOUER, ANDREA; ANDRE, MARCOS ROGERIO; GONCALVES, LUIZ RICARDO; LUZZI, MAYARA DE CASSIA; DE OLIVEIRA, JULIANA PAULA; RODRIGUES, ADRIANA CARLOS; VARANI, ALESSANDRO DE MELO; OLIVEIRA DE MIRANDA, VITOR FERNANDES; PERLES, LIVIA; WERTHER, KARIN; et al. Hepatozoon caimani em Caiman crocodilus yacare (Crocodylia, Alligatoridae) do Norte do Pantanal, Brasil. Revista Brasileira de Parasitologia Veterinária, v. 26, n. 3, p. 352-358, JUL-SEP 2017. (15/14896-1)

CALIFRE DE MELLO, VICTORIA VALENTE; DE SOUZA RAMOS, INALDA ANGELICA; HERRERA, HEITOR MIRAGLIA; MENDES, NATALIA SERRA; CALCHI, ANA CLAUDIA; VILELA CAMPOS, JOAO BOSCO; MACEDO, GABRIEL CARVALHO; ALMEIDA ALVES, JOAO VITOR; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Occurrence and genetic diversity of hemoplasmas in beef cattle from the Brazilian Pantanal, an endemic area for bovine trypanosomiasis in South America. COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, v. 66, OCT 2019. (15/14896-1)

MARQUES DE SOUSA, KEYLA CARSTENS; HERRERA, HEITOR MIRAGLIA; ROCHA, FABIANA LOPES; COSTA, FRANCISCO BORGES; MARTINS, THIAGO FERNANDES; LABRUNA, MARCELO BAHIA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Rickettsia spp. among wild mammals and their respective ectoparasites in Pantanal wetland, Brazil. TICKS AND TICK-BORNE DISEASES, v. 9, n. 1, p. 10-17, JAN 2018. (15/14896-1, 13/13186-5)

MARQUES DE SOUSA, KEYLA CARSTENS; DO AMARAL, RENAN BRESSIANINI; HERRERA, HEITOR MIRAGLIA; SANTOS, FILIPE MARTINS; MACEDO, GABRIEL CARVALHO; ESTRELA DE ANDRADE PINTO, PEDRO CORDEIRO; BARROS-BATTESTI, DARCI MORAES; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Genetic Diversity of Bartonella spp. in Wild Mammals and Ectoparasites in Brazilian Pantanal. MICROBIAL ECOLOGY, v. 76, n. 2, p. 544-554, AUG 2018. (15/03262-1, 15/14896-1, 13/13186-5)

PERLES, L.; ROQUE, A. L. R.; D'ANDREA, P. S.; LEMOS, E. R. S.; SANTOS, A. F.; MORALES, A. C.; MACHADO, R. Z.; ANDRE, M. R.. Genetic diversity of Hepatozoon spp. in rodents from Brazil. SCIENTIFIC REPORTS, v. 9, JUL 12 2019. (15/14896-1)

IKEDA, P.; SEKI, M. C.; CARRASCO, A. O. T.; RUDIAK, L. V.; MIRANDA, J. M. D.; GONCALVES, S. M. M.; HOPPE, E. G. L.; ALBUQUERQUE, A. C. A.; TEIXEIRA, M. M. G.; PASSOS, C. E.; et al. Evidence and molecular characterization of Bartonella spp. and hemoplasmas in neotropical bats in Brazil. EPIDEMIOLOGY AND INFECTION, v. 145, n. 10, p. 2038-2052, JUL 2017. (15/14896-1, 15/04773-0)

COSTA DE OLIVEIRA BRAGA, MARIA DO SOCORRO; PEREIRA, JOSE GOMES; FERNANDES, SIMONE DE JESUS; LOPES MARQUES, INGRID CAROLINNE; DE JESUS, RENATA PASSOS; FERREIRA, GLEYCIANNY SANTOS; XAVIER, DANIELE ROSA; BENEVENUTE, JYAN LUCAS; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. Molecular detection of Anaplasmataceae agents in Dasyprocta azarae in northeastern Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 27, n. 1, p. 7-pg., 2018-01-01. (15/14896-1)

TOLEDO, MARIANA APARECIDA; LEITE, ALEXANDRO IRIS; GONCALVES, LUIZ RICARDO; MARQUES DE SOUSA, KEYLA CARSTENS; DO AMARAL, RENAN BRESSIANINI; PINHEIRO DA SILVA, GLAUCENYRA CECILIA; MACHADO, ROSANGELA ZACARIAS; ANDRE, MARCOS ROGERIO. High occurrence of Mycoplasma suis infection in swine herds from non-technified farms in Mossoro, state of Rio Grande do Norte, Northeastern Brazil. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 25, n. 4, p. 4-pg., 2016-09-01. (15/14896-1, 14/00931-7)