Characterization of the optimal hiPSC-derived cardiac cell population for heart regeneration after myocardial infarction

• Grant number: 15/50216-5
• Support Opportunities: Research Grants - Research Partnership for Technological Innovation - PITE
• Start date: August 01, 2016
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Agreement: AstraZeneca/MedImmune
• Principal Investigator: José Eduardo Krieger
• Grantee: José Eduardo Krieger
• Host Institution: Instituto do Coração Professor Euryclides de Jesus Zerbini (INCOR). Hospital das Clínicas da Faculdade de Medicina da USP (HCFMUSP). Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Company: AstraZeneca do Brasil Ltda
• City: São Paulo
• Pesquisadores principais: Clara Steichen ; Rafael Dariolli ; Thayane Antoniolli Crestani

Abstract

The main goal of this project is to characterize the optimal hiPSC-derived cell population for cardiac regeneration after MI, using a preclinical model of Ml in pigs. First, human iPSCs will be generated from either human fibroblasts or urine samples using a non-integrative reprogramming strategy based on episomal transfection. hiPSCs will be characterized to ensure they show the typical features of pluripotent stem cells. Then, we will differentiate them toward the cardiac lineage using an efficient modified protocol based on small molecules enabling Wnt pathway control. The second aim of this project is a thoroughly characterization of cell populations at different stages of differentiation, from immature mesoderm cells to mature cardiomyocytes (targeting committed, although not fully differentiated cardiomyocytes). We will then perform a pilot study using a rat model of myocardial infarction to identify the optimal cell (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ZOGBI, CAMILA; OLIVEIRA, NATHALIA C.; LEVY, DEBORA; BYDLOWSKI, SERGIO P.; BASSANEZE, VINICIUS; NERI, ELIDA A.; KRIEGER, JOSE E.. Beneficial effects of IL-4 and IL-6 on rat neonatal target cardiac cells. SCIENTIFIC REPORTS, v. 10, n. 1, JUL 23 2020. (15/50216-5, 17/17296-0, 17/07024-3, 13/17368-0)

JENSEN, LEONARDO; NERI, ELIDA; BASSANEZE, VINICIUS; DE ALMEIDA OLIVEIRA, NATHALIA C.; DARIOLLI, RAFAEL; TURACA, LAURO T.; LEVY, DEBORA; VERONEZ, DOUGLAS; FERRAZ, MARIANA S. A.; ALENCAR, ADRIANO M.; et al. Integrated molecular, biochemical, and physiological assessment unravels key extraction method mediated influences on rat neonatal cardiomyocytes. Journal of Cellular Physiology, v. 233, n. 7, p. 5420-5430, JUL 2018. (16/50439-7, 13/17368-0, 11/19678-1, 15/50216-5)

MUNOZ, JUAN J. A. M.; DARIOLLI, RAFAEL; DA SILVA, CAIO MATEUS; NERI, ELIDA A.; VALADAO, IURI C.; TURACA, LAURO THIAGO; LIMA, VANESSA M.; PERES DE CARVALHO, MARIANA LOMBARDI; VELHO, MARILIZA R.; SOBIE, ERIC A.; et al. Time-regulated transcripts with the potential to modulate human pluripotent stem cell-derived cardiomyocyte differentiation. STEM CELL RESEARCH & THERAPY, v. 13, n. 1, p. 27-pg., 2022-09-02. (16/07541-5, 14/50889-7, 13/17368-0, 15/50216-5, 14/24577-8)

CRESTANI, THAYANE; STEICHEN, CLARA; NERI, ELIDA; RODRIGUES, MARILIZA; FONSECA-ALANIZ, MIRIAM HELENA; ORMROD, BETH; HOLT, MARK R.; PANDEY, PRAGATI; HARDING, SIAN; EHLER, ELISABETH; et al. Electrical stimulation applied during differentiation drives the hiPSC-CMs towards a mature cardiac conduction-like cells. Biochemical and Biophysical Research Communications, v. 533, n. 3, p. 376-382, DEC 10 2020. (13/17368-0, 16/11321-0, 14/14086-7, 18/16860-2, 15/50216-5)

DA SILVA, AGATHA RIBEIRO; NERI, ELIDE A.; TURACA, LAURO THIAGO; DARIOLLI, RAFAEL; FONSECA-ALANIZ, MIRIAM H.; SANTOS-MIRANDA, ARTUR; ROMAN-CAMPOS, DANILO; VENTURINI, GABRIELA; KRIEGER, JOSE E.. NOTCH1 is critical for fibroblast-mediated induction of cardiomyocyte specialization into ventricular conduction system-like cells in vitro. SCIENTIFIC REPORTS, v. 10, n. 1, p. 18-pg., 2020-09-30. (19/21304-4, 14/24577-8, 18/22830-9, 15/50216-5, 17/05829-4, 13/17368-0, 14/09861-1)