Gender differences in ontogenetic development, behavior and energy metabolism in rats subjected to neonatal anoxia: focus on the hippocampus, hypothalamus, leptin and glia cels

• Grant number: 15/18415-8
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2016
• End date: October 31, 2018
• Field of knowledge: Biological Sciences - Morphology - Anatomy
• Principal Investigator: Maria Inês Nogueira
• Grantee: Maria Inês Nogueira
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The neonatal anoxia, a global clinical problem has been studied in Neuroscience Laboratory ICB-USP, with a model developed, adapted and already validated. These studies showed deleterious effects in the hippocampus of rats sbjected to neonatal anoxia that persist into adulthood, such as higher immunoreactivity astrocytes, neuronal death in the hippocampus by apoptosis and necrosis, altered neurogenesis rate, reduced brain volume, which corroborates spatial memory impairment and an increase in behaviors like anxiety, age dependent, and loss of spatial memory. In anoxic animals was observed weight gain compared to control, as well as increased rostrocaudal and nasoanal diameters, already evident in the first weeks of breastfeeding. These data suggest involvement of the hypothalamus structure directly related to energy metabolism and also with somatic parameters, suggesting further possible gender differences in the responses to anoxic stimulation. These are the questions that this study plans to address with somatic maturation assessment, sensorimotor and behavioral as well as immunohistochemistry and stereology of neuronal activation, OBRb the leptin receptor and glial response: astrocytes, microglia and oligodendrocytes. Understanding the structures and mechanisms involved in this neonatal anoxia model aims to contribute to the identification of windows to possible interference or actions that minimize or reduce symptoms from the effects of this injury is this socio-educational and public health concern. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KUMAR, AMRITA JHA; TAKADA, SILVIA HONDA; MOTTA-TEIXEIR, LIVIA CLEMENTE; LEE, VITOR YONAMINE; XAVIER, GILBERTO FERNANDO; NOGUEIRA, MARIA INES. Sex differences in somatic and sensory motor development after neonatal anoxia in Wistar rats. Behavioural Brain Research, v. 333, p. 242-250, AUG 30 2017. (14/22313-3, 15/18415-8)

HELOU, AMMIR YACOUB; MARTINS, DANIEL OLIVEIRA; ARRUDA, BRUNA PETRUCELLI; DE SOUZA, MATHEUS CERUSSI; CRUZ-OCHOA, NATALIA ANDREA; NOGUEIRA, MARIA INES; CHACUR, MARUCIA. Neonatal anoxia increases nociceptive response in rats: Sex differences and lumbar spinal cord and insula alterations. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, v. 81, n. 8, AUG 2021. (15/18415-8, 14/22313-3, 17/05218-5, 15/24256-0)

HELOU, AMMIR YACOUB; CRUZ OCHOA, NATALIA ANDREA; FELICIO, LUCIANO FREITAS; NOGUEIRA, MARIA INES. Neonatal Anoxia Impacts Rats' Maternal Behavior and Offspring Development due to Ultrasonic Vocalization's Impairment. DEVELOPMENTAL NEUROSCIENCE, v. 44, n. 6, p. 14-pg., 2022-01-01. (17/05218-5, 15/18415-8)

KUMAR, AMRITA JHA; MARTINS, DANIEL OLIVEIRA; ARRUDA, BRUNA PETRUCELLI; LEE, VITOR YONAMINE; CHACUR, MARUCIA; NOGUEIRA, MARIA INES. Impairment of nociceptive responses after neonatal anoxia correlates with somatosensory thalamic damage: A study in rats. Behavioural Brain Research, v. 390, JUL 15 2020. (15/18415-8, 14/22313-3)

VASQUEZ MATSUDA, VICTOR DANIEL; BUSTELO TEJADA, MARTIN; MOTTA-TEIXEIRA, LIVIA CLEMENTE; IKEBARA, JULIANE MIDORI; CARDOSO, DEBORA STERZECK; MACHADO-NILS, ALINE VILAR; LEE, VITOR YONAMINE; DICCINI, ISABELLE; ARRUDA, BRUNA PETRUCELLI; MARTINS, PAMELA PINHEIRO; et al. Impact of neonatal anoxia and hypothermic treatment on development and memory of rats. Experimental Neurology, v. 340, JUN 2021. (17/26439-0, 18/14072-7, 18/06731-0, 15/18415-8, 16/18941-4)

KUMAR, AMRITA JHA; MOTTA-TEIXEIRA, LIVIA CLEMENTE; TAKADA, SILVIA HONDA; YONAMINE-LEE, VITOR; MACHADO-NILS, ALINE VILAR; XAVIER, GILBERTO FERNANDO; NOGUEIRA, MARIA INES. Behavioral, cognitive and histological changes following neonatal anoxia: Male and female rats' differences at adolescent age. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, v. 73, p. 50-58, APR 2019. (14/22313-3, 16/18941-4, 15/18415-8)

CRUZ-OCHOA, NATALIA ANDREA; ESPERANZA OCHOA-AMAYA, JULIETA; LORENA PULECIO, SANDY; XAVIER, GILBERTO FERNANDO; NOGUEIRA, MARIA INES. Neonatal anoxia impairs long-term energy metabolism and somatic development of Wistar rats. INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, v. 79, p. 76-85, DEC 2019. (15/18415-8, 10/52068-0)

KUMAR, AMRITA JHA; HELOU, AMMIR YACOUB; PETRUCELLI, BRUNA ARRUDA; XAVIER, GILBERTO FERNANDO; MARTINS, DANIEL OLIVEIRA; CHACUR, MARUCIA; NOGUEIRA, MARIA INES. Sensorimotor development of male and female rats subjected to neonatal anoxia. DEVELOPMENTAL PSYCHOBIOLOGY, v. 64, n. 7, p. 10-pg., 2022-11-01. (14/22313-3, 15/18415-8)