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Role of Hydrogen Sulphide (H2S) on the ventilatory responses to hypercapnia

Grant number: 15/24950-3
Support type:Regular Research Grants
Duration: April 01, 2016 - March 31, 2018
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Luiz Guilherme de Siqueira Branco
Grantee:Luiz Guilherme de Siqueira Branco
Home Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Hydrogen sulfide (H2S), recently described as the third member of the group of gaseous transmitters, is produced endogenously by metabolic pathways with predominant participation of two enzymes: cystathionine beta-synthase (CBS ) and cystathionine gamma-lyase (CSE ). The presence and participation of CBS and CSE for generating H2S in response to hypoxia have been reported in the carotid body. However, the presence and distribution of these enzymes have not been described in specific regions of the brainstem, in particular of medullary raphe nuclei, which play an important role in ventilatory responses to hypercapnia. Since it is not known the role of endogenous H2S in the bulbar raphe on the ventilatory responses to hypercapnia, adding to the therapeutic potential for the use of this gas in clinical practice, it is extremely important to study the central mechanisms by which H2S participates in the control respiratory, in particular the aspects relating to the central chemoreception CO2/pH. Thus, this proposal aims to assess the role of H2S in the bulbar raphe on the ventilatory responses to hypercapnia. This will have three specific objectives: 1) to study the effect of microinjection in the bulbar raphe inhibitor CBS enzyme and H2S donor in ventilatory responses to hypercapnia (ventilation, O2 consumption and body temperature), 2 ) by means of immunohistochemistry, to analyze the presence and distribution of CBS enzyme in the region of bulbar raphe, as well as its co- location with serotonergic neurons in this region, which is important in central chemoreception and 3) to analyze the effect of exposure to hypercapnia on the endogenous production of H2S in the bulbar raphe. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SORIANO, RENATO N.; BRAGA, SARA P.; BREDER, JESSICA S. C.; BATALHAO, MARCELO E.; OLIVEIRA-PELEGRIN, GABRIELA R.; FERREIRA, LUIZ FERNANDO R.; ROCHA, MARIA JOSE A.; CARNIO, EVELIN C.; BRANCO, LUIZ G. S. Endogenous peripheral hydrogen sulfide is propyretic: its permissive role in brown adipose tissue thermogenesis in rats. Experimental Physiology, v. 103, n. 3, p. 397-407, MAR 1 2018. Web of Science Citations: 5.
SABINO, JOAO PAULO J.; SORIANO, RENATO N.; DONATTI, ALBERTO F.; FERNANDEZ, RODRIGO RESTREPO; KWIATKOSKI, MARCELO; FRANCESCATO, HELOISA D. C.; COIMBRA, TEREZILA M.; BRANCO, LUIZ G. S. Involvement of endogenous central hydrogen sulfide (H2S) in hypoxia-induced hypothermia in spontaneously hypertensive rats. Canadian Journal of Physiology and Pharmacology, v. 95, n. 2, p. 157-162, FEB 2017. Web of Science Citations: 1.
NOGUEIRA, JONATAS E.; SORIANO, RENATO N.; FERNANDEZ, RODRIGO A. R.; FRANCESCATO, HELOISA D. C.; SAIA, RAFAEL S.; COIMBRA, TEREZILA M.; ANTUNES-RODRIGUES, JOSE; BRANCO, LUIZ G. S. Effect of Physical Exercise on the Febrigenic Signaling is Modulated by Preoptic Hydrogen Sulfide Production. PLoS One, v. 12, n. 1 JAN 24 2017. Web of Science Citations: 5.

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