Cell signaling in Trypanosoma during host-parasite interaction

• Grant number: 15/22031-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: April 01, 2016
• End date: May 31, 2020
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Sergio Schenkman
• Grantee: Sergio Schenkman
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Beatriz Amaral de Castilho ; Bradford Scott Mcgwire ; Mark C. Field
• Associated scholarship(s): 18/12886-7 - The role of protein kinase AMPK in the Trypanosoma cruzi differentiation, BP.MS; 18/07766-2 - TcCyp19 caracterization and evaluation of its role during cell infection by Trypanosoma cruzi, BP.DD; 17/19303-4 - Effect of Trypanosoma cruzi cyclophilin 19 on mammalian cells, BP.IC; + associated scholarships 17/02496-4 - Role of protein and sirtuins acetylation in T. cruzi infection process, BP.PD; 17/02416-0 - Protein secretion in Trypanosoma, BP.PD; 16/20743-6 - Characterizing protein kinase translation initiation factor 2 of Trypanosoma cruzi, BP.IC - associated scholarships

Abstract

Response mechanisms of protozoan parasites to the situations found in the hosts is not well known. This understanding can help to identify new chemotherapeutic targets and on the comprehension of the mechanisms that lead to pathogenesis. Our group has studied for last several years, how protein synthesis is regulated in Trypanosoma under stressing conditions. In this thematic project, we intend to continue these studies and extend our approach to other projects already initiated by our group. They will include the characterization and the role of protein deacetylases and cyclophilins (enzymes that isomerize proline bonds in proteins) of Trypanosoma cruzi, the agent of Chagas disease. We also intend to start a novel study of proteins involved in secretion of proteins anchored by glycosylphosphatidylinositol in Trypanosoma, comprising the major surface proteins of the parasite. Here, we are presenting four interconnected subprojects that will be conducted by a group of 2 post-doctoral fellows and 3 graduate students, which will allow understanding how these parasites control the different types of stress to survive in their hosts. (AU)

Scientific publications

(10)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MATUTINO BASTOS, TANIRA; BOTELHO PEREIRA SOARES, MILENA; HADDAD FRANCO, CAIO; ALCANTARA, LAURA; ANTONINI, LORENZO; SABATINO, MANUELA; MAUTONE, NICOLA; HOLANDA FREITAS-JUNIOR, LUCIO; MORAES, CAROLINA BORSOI; RAGNO, RINO; et al. Identification of Inhibitors to Trypanosoma cruzi Sirtuins Based on Compounds Developed to Human Enzymes. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 10, MAY 2020. (18/09948-0, 15/22031-0)

MOREIRA, DOUGLAS DE SOUZA; DUARTE, ANA PAULA; MIRSKY PAIS, FABIANO SVIATOPOLK; DA SILVA-PEREIRA, ROSIANE APARECIDA; ROMANHA, ALVARO JOSE; SCHENKMAN, SERGIO; FONSECA MURTA, SILVANE MARIA. Overexpression of eukaryotic initiation factor 5A (eIF5A) affects susceptibility to benznidazole in Trypanosoma cruzi populations. Memórias do Instituto Oswaldo Cruz, v. 113, n. 9, 2018. (15/22031-0)

DE LIMA STEIN, MARIANA LEDO; ICIMOTO, MARCELO YUDI; DE CASTRO LEVATTI, ERICA VALADARES; OLIVEIRA, VITOR; STRAUS, ANITA HILDA; SCHENKMAN, SERGIO. Characterization and role of the 3-methylglutaconyl coenzyme A hidratase in Trypanosoma brucei. Molecular and Biochemical Parasitology, v. 214, p. 36-46, JUN 2017. (15/22031-0, 11/51973-3)

LEITE, ARIELY BARBOSA; SEVERO GOMES, ANTONIEL AUGUSTO; DE CASTRO NASCIMENTO SOUSA, ANA CAROLINE; DE MATTOS FONTES, MARCOS ROBERTO; SCHENKMAN, SERGIO; MORETTI, NILMAR SILVIO. Effect of lysine acetylation on the regulation of Trypanosoma brucei glycosomal aldolase activity. Biochemical Journal, v. 477, n. 9, p. 1733-1744, MAY 2020. (18/09948-0, 15/22031-0)

DOS SANTOS MOURA, LEILA; SANTANA NUNES, VINICIUS; GOMES, ANTONIEL A. S.; SOUSA, ANA CAROLINE DE CASTRO NASCIMENTO; FONTES, MARCOS R. M.; SCHENKMAN, SERGIO; MORETTI, NILMAR SILVIO. Mitochondrial Sirtuin TcSir2rp3 Affects TcSODA Activity and Oxidative Stress Response in Trypanosoma cruzi. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, NOV 11 2021. (14/03714-7, 20/07870-4, 18/09948-0, 15/22031-0)

JHA, BIJAY KUMAR; VARIKUTI, SANJAY; VERMA, CHAITENYA; SHIVAHARE, RAHUL; BISHOP, NICHOLAS; DOS SANTOS, GREGORY P.; MCDONALD, JACQUELYN; SUR, AAKASH; MYLER, PETER J.; SCHENKMAN, SERGIO; et al. Immunization with a Trypanosoma cruzi cyclophilin-19 deletion mutant protects against acute Chagas disease in mice. NPJ VACCINES, v. 8, n. 1, p. 15-pg., 2023-04-25. (15/22031-0)

BASTOS, TANIRA MATUTINO; RUSSO, HELENA MANNOCHIO; MORETTI, NILMAR SILVIO; SCHENKMAN, SERGIO; MARCOURT, LAURENCE; GUPTA, MAHABIR PRASHAD; WOLFENDER, JEAN-LUC; QUEIROZ, EMERSON FERREIRA; PEREIRA SOARES, MILENA BOTELHO. Chemical Constituents of Anacardium occidentale as Inhibitors of Trypanosoma cruzi Sirtuins. Molecules, v. 24, n. 7, APR 3 2019. (15/22031-0, 18/09948-0)

ROUT, MICHAEL P.; OBADO, SAMSON O.; SCHENKMAN, SERGIO; FIELD, MARK C.. Specialising the parasite nucleus: Pores, lamins, chromatin, and diversity. PLOS PATHOGENS, v. 13, n. 3, MAR 2017. (11/51973-3, 15/22031-0)

MORETTI, NILMAR SILVIO; CESTARI, IGOR; ANUPAMA, ATASHI; STUART, KEN; SCHENKMAN, SERGIO. Comparative Proteomic Analysis of Lysine Acetylation in Trypanosomes. JOURNAL OF PROTEOME RESEARCH, v. 17, n. 1, p. 374-385, JAN 2018. (11/51973-3, 15/22031-0, 13/20074-9)

MORETTI, NILMAR SILVIO; MORTARA, RENATO ARRUDA; SCHENKMAN, SERGIO. Trypanosoma cruzi. Trends in Parasitology, v. 36, n. 4, p. 2-pg., 2020-04-01. (15/22031-0, 16/15000-4)