Development of biomarkers and Leishmania infantum strains

• Grant number: 15/23767-0
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2016
• End date: November 30, 2018
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Selma Giorgio
• Grantee: Selma Giorgio
• Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Daniel Ruiz Abánades ; Marcos Nogueira Eberlin

Abstract

Leishmaniasis is a group of neglected and endemic diseases that occur in tropical and subtropical countries. Leishmania protozoan parasite infect mononuclear phagocytes; and pathological manifestations are cutaneous and mucosal lesions and visceral disease in humans. Different methods are used for diagnosis, but none with high sensitivity and specificity, there is no vaccine for large-scale use, and there are reports of treatment failures and drug resistance. Despite the difficulties, justifying new alternatives and approaches to prognosis, diagnosis, treatment and prevention of leishmaniasis, in vitro model (cultured macrophages infected with Leishmania) and in vivo model (inbred mice) are excellent tools for immunological and biochemical studies. This project is divided into two sub-projects: 1) biomarkers research: assessment of biomolecules with differential expression during mouse infection with L. infantum and L. amazonensis, by MALDI imaging mass spectrometry and immunohistochemistry; 2) development of transgenic strains of L. infantum: transfected parasites that express proteins / toxic polypeptides only in amastigote form i.e. suicide parasites die when differentiate into amastigotes, and are able to proliferate and infect during promastigote stage. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NEGRAO, FERNANDA; GIORGIO, SELMA; EBERLIN, MARCOS NOGUEIRA; YATES, III, JOHN R.. Comparative Proteomic Analysis of Murine Cutaneous Lesions Induced by Leishmania amazonensis or Leishmania major. ACS INFECTIOUS DISEASES, v. 5, n. 8, p. 1295-1305, AUG 2019. (15/23767-0, 17/22651-4)

NEGRAO, FERNANDA; ABANADES, DANIEL R.; JAEEGER, CAROLINE F.; ROCHA, DANIELE F. O.; BELAZ, KATIA R. A.; GIORGIO, SELMA; EBERLIN, MARCOS N.; ANGOLINI, CELIO F. F.. Lipidomic alterations of in vitro macrophage infection by L-infantum and L-amazonensis. MOLECULAR BIOSYSTEMS, v. 13, n. 11, p. 2401-2406, NOV 2017. (16/11517-2, 15/23767-0, 12/07206-0, 13/11100-6, 10/51677-2)

ZORGI, NAHIARA ESTEVES; ARRUDA, LEONARDO V.; PALADINE, IZADORA; ROQUE, GUILHERME A. S.; ARAUJO, THALITA F.; BROCCHI, MARCELO; BARRAL, MANOEL; SANCHIZ, AFRICA; MARIA REQUENA, JOSE; ABANADES, DANIEL R.; et al. Leishmania infantum transfected with toxic plasmid induces protection in mice infected with wild type L. infantum or L. amazonensis. Molecular Immunology, v. 127, p. 95-106, NOV 2020. (18/23302-6, 19/06322-6, 15/23767-0, 16/11539-6)

NEGRAO, FERNANDA; FERNANDEZ-COSTA, CAROLINA; ZORGI, NAHIARA; GIORGIO, SELMA; EBERLIN, MARCOS NOGUEIRA; YATES, III, JOHN R.. Label-Free Proteomic Analysis Reveals Parasite-Specific Protein Alterations in Macrophages Following Leishmania amazonensis, Leishmania major, or Leishmania infantum Infection. ACS INFECTIOUS DISEASES, v. 5, n. 6, p. 851-862, JUN 2019. (15/23767-0, 17/22651-4)

NEGRAO, FERNANDA; DIEDRICH, JOLENE K.; GIORGIO, SELMA; EBERLIN, MARCOS N.; YATES, III, JOHN R.. Tandem Mass Tag Proteomic Analysis of in Vitro and in Vivo Models of Cutaneous Leishmaniasis Reveals Parasite-Specific and Nonspecific Modulation of Proteins in the Host. ACS INFECTIOUS DISEASES, v. 5, n. 12, SI, p. 2136-2147, DEC 2019. (15/23767-0, 17/22651-4)

NEGRAO, FERNANDA; DIEDRICH, JOLENE K.; GIORGIO, SELMA; EBERLIN, MARCOS N.; YATES, JOHN R., III. Tandem Mass Tag Proteomic Analysis of in Vitro and in Vivo Models of Cutaneous Leishmaniasis Reveals Parasite-Specific and Nonspecific Modulation of Proteins in the Host. ACS INFECTIOUS DISEASES, v. 5, n. 12, p. 23-pg., 2019-12-01. (15/23767-0, 17/22651-4)

JAEGGER, C. F.; NEGRAO, F.; ASSIS, D. M.; BELAZ, K. R. A.; ANGOLINI, C. F. F.; FERNANDES, A. M. A. P.; SANTOS, V. G.; PIMENTEL, A.; ABANADES, D. R.; GIORGIO, S.; et al. MALDI MS imaging investigation of the host response to visceral leishmaniasis. MOLECULAR BIOSYSTEMS, v. 13, n. 10, p. 1946-1953, OCT 2017. (16/11517-2, 15/23767-0, 13/11100-6, 12/07206-0)

NEGRAO, FERNANDA; ROCHA, DANIELE F. DE O.; JAEEGER, CAROLINE F.; ROCHA, FRANCISCA J. S.; EBERLIN, MARCOS N.; GIORGIO, SELMA. Murine cutaneous leishmaniasis investigated by MALDI mass spectrometry imaging. MOLECULAR BIOSYSTEMS, v. 13, n. 10, p. 2036-2043, OCT 2017. (16/11517-2, 15/23767-0)

NEGRAO, FERNANDA; EBERLIN, MARCOS NOGUEIRA; GIORGIO, SELMA. Proteomic approaches for drug discovery against tegumentary leishmaniasis. BIOMEDICINE & PHARMACOTHERAPY, v. 95, p. 577-582, NOV 2017. (16/11517-2, 15/23767-0)

BENINCASA, JULIA C.; DE FREITAS FILHO, LUIZ H.; CARNEIRO, GIANE D.; SIELSKI, MICHELI S.; GIORGIO, SELMA; WERNECK, CLAUDIO C.; VICENTE, CRISTINA P.. Hyperbaric oxygen affects endothelial progenitor cells proliferation in vitro. Cell Biology International, v. 43, n. 2, p. 136-146, FEB 2019. (15/23767-0)

ORTEGA, VANESSA; RADAIC, ALLAN; DE JESUS, MARCELO BISPO; DE PAULA, ENEIDA; GIORGIO, SELMA. Improved efficacy of meglumine antimoniate incorporated in anionic liposomes against Leishmania infantum infecting canine macrophages. Journal of Pharmacy and Pharmacology, v. 74, n. 6, p. 9-pg., 2021-07-21. (14/12443-7, 15/23767-0, 13/01536-1)