Cytotoxicity and photo-cytotoxicity of nitrosyl ruthenium anticancer drug in aqueous solution or incorporated in a drug delivery system.an innovative purpose for metal based drug

• Grant number: 15/50101-3
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2016 - May 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Roberto Santana da Silva
• Grantee: Roberto Santana da Silva
• Principal researcher abroad: Alexandra R. Fernandes
• Institution abroad: Universidade Nova de Lisboa, Portugal
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 13/08851-0 - Ruthenium phthalocyanine complexes as prototypes for metallo-drugs photochemically active: photobiological studies, theranostic effect and metabolism evaluation, AP.R

Abstract

The objective of this project is the study of energy transfer between donor electron species and complex trans- [RuNO (pc-R) (N02)] (PC-R is modified phthalocyanine) as producing agents of reactive oxygen species (ROS) and nitrogen (ERONs) by light irradiation at 500 nm to 700 nm region. Measurements of NO, singlet oxygen and its derivatives shall be determined on the basis of different concentrations of oxygen and the cytotoxic potential assessed in cancer cell lines. Photochemical studies will be conducted both in deaerated medium as a function of oxygen concentration. Biochemical processes related to complex interaction of ruthenium-cell will be evaluated for interaction with ruthenium cell surface proteoglycans by labeling with fluorophores; Akt phosphorylation and mitogen-activated protein kinase (MAPK); Evaluation of AMPK (AMP-activated protein kinase), JNK (c-jun terminal kinase-N) and ATF3 (3 transcription factor activation), in response to treatment with ruthenium complexes. (AU)