Human cell culture model to investigate the effect of inhaled fragrances on airway

• Grant number: 16/02782-4
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2016
• End date: August 31, 2019
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Mariangela Macchione
• Grantee: Mariangela Macchione
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Carla Máximo Prado ; Fernanda Magalhães Arantes Costa ; Kelly Yoshizaki ; Luís Fernando Amato Lourenço ; Monique Matsuda ; Paulo Hilário Nascimento Saldiva ; Rosimeire Aparecida Roela ; Thais Mauad

Abstract

For ethical and regulatory reasons, in vitro tests for scoring potential toxicities of cosmetics are essential. Despite estimates indicating that sensitivity to fragrance products is common, the frequency of mucosal symptoms elicited by exposure to these products in the general population is unknown. Here, we will use a co-culture of BEAS-2B and macrophage cells to study effects of fragrances to know their sensitization effects on respiratory tract cells. We will study cytotoxicity through the MTT assay, LDH assay, Annexin V-propidium iodide staining (apoptosis assay), time and concentration of the fragrance that produced inflammatory mediators and CYPs. We will conduct measures using real-time reverse transcriptasepolymerase chain reaction, Western blot, ELISA and flow cytometry. (AU)