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Analysis of electrophysiological and morphofunctional properties of neurons in the central nucleus of inferior colliculus related to genesis and spreading of sound-induced convulsive seizures

Grant number: 15/22327-7
Support type:Research Grants - Young Investigators Grants
Duration: September 01, 2016 - August 31, 2020
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Alexandra Olimpio Siqueira Cunha
Grantee:Alexandra Olimpio Siqueira Cunha
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Assoc. researchers:Norberto Garcia Cairasco ; Ricardo Mauricio Xavier Leão
Associated scholarship(s):19/18428-3 - Morphological alterations of neurons from the central nucleus of inferior colliculus in Wistar and war strains submitted to repetitive sound stimulation, BP.IC
16/18226-3 - The role of inferior colliculus as an integrative structure for auditory information and as a site for initialization and spread of audiogenic seizures: an eletrophysiological and morpho-functional study, BP.JP

Abstract

Virtually all auditory information arrives in inferior colliculus in a tonotopic manner from primary auditory structures, as well as from prosencephalic structures such as: thalamus, amygdala and auditory cortex. Intracollicular circuits integrate the received information and send projections to extracollicular ascendant and descendant pathways. The hyper activation of collicular circuits is apparently involved in the seizures observed upon a high intensity sound stimulus. The so called audiogenic seizures may occur in susceptible animals; generally rodents genetically selected and basically consist of the hyper-activation and hyper-synchronization of groups of neurons. Interestingly, the repetition of the sound stimulus may lead to the spread of the epileptiform activity to limbic areas, leading to behaviour and electroencephalographic alterations similar to those observed in models of temporal lobe epilepsy. The WAR strain (Wistar audiogenic rat) is greatly susceptible to audiogenic seizures and can be used to assess circuits involved with the genesis of the seizures in auditory brain stem and recruitment of limbic areas. Therefore, and based on the major role of inferior colliculus in auditory processing in general, our hypothesis is that specific intracollicular circuits are capable of generate and spread epileptiform activity when hyper-activated. Thus, the present study aims at the investigation of alterations in neuronal membrane properties, synaptic plasticity, intrinsic and extrinsic connectivity and auditory processing of neurons and pathways to and from inferior colliculus using whole cell patch clamp in vivo and optogenetics. The importance of this study is based mostly on the lack of data on intracollicular circuits, as well as in the continuous need for knowledge in neuronal processing that lead to epileptiform activity during epileptic seizures. (AU)

Scientific publications (8)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE DEUS, JUNIA L.; AMORIM, MATEUS R.; RIBEIRO, ALINE B.; BARCELLOS-FILHO, PROCOPIO C. G.; CEBALLOS, CESAR C.; BRANCO, LUIZ GUILHERME S.; CUNHA, ALEXANDRA O. S.; LEAO, RICARDO M. Loss of Brain-Derived Neurotrophic Factor Mediates Inhibition of Hippocampal Long-Term Potentiation by High-Intensity Sound. Cellular and Molecular Neurobiology, MAY 2020. Web of Science Citations: 0.
PENA, RODRIGO F. O.; CEBALLOS, CESAR CELIS; DE DEUS, JUNIA LARA; ROQUE, ANTONIO CARLOS; GARCIA-CAIRASCO, NORBERTO; LEAO, RICARDO MAURICIO; SIQUEIRA CUNHA, ALEXANDRA OLIMPIO. Modeling Hippocampal CA1 Gabaergic Synapses of Audiogenic Rats. International Journal of Neural Systems, v. 30, n. 5 MAY 2020. Web of Science Citations: 0.
DE DEUS, JUNIA LARA; AMORIM, MATEUS RAMOS; GAMA DE BARCELLOS FILHO, PROCOPIO CLEBER; CORTES DE OLIVEIRA, JOSE ANTONIO; BATALHAO, MARCELO EDUARDO; GARCIA-CAIRASCO, NORBERTO; CARNIO, EVELIN CAPELLARI; LEAO, RICARDO MAURICIO; DE SIQUEIRA BRANCO, LUIZ GUILHERME; SIQUEIRA CUNHA, ALEXANDRA OLIMPIO. Inflammatory markers in the hippocampus after audiogenic kindling. Neuroscience Letters, v. 721, MAR 16 2020. Web of Science Citations: 0.
SIQUEIRA CUNHA, ALEXANDRA OLIMPIO; MORADI, MARZIEH; DE DEUS, JUNIA LARA; CEBALLOS, CESAR CELIS; MOREIRA BENITES, NIKOLLAS; GAMA DE BARCELLOS FILHO, PROCOPIO CLEBER; CORTES DE OLIVEIRA, JOSE ANTONIO; GARCIA-CAIRASCO, NORBERTO; LEAO, RICARDO. Alterations in brainstem auditory processing, the acoustic startle response and sensorimotor gating of startle in Wistar audiogenic rats (WAR), an animal model of reflex epilepsies. Brain Research, v. 1727, JAN 15 2020. Web of Science Citations: 0.
PORTO DECHANDT, CARLOS ROBERTO; FERRARI, GUSTAVO DUARTE; DOS SANTOS, JONATHAS RODRIGO; CORTES DE OLIVEIRA, JOSE ANTONIO; PATRICIO SILVA-JR, RUI MILTON; SIQUEIRA CUNHA, ALEXANDRA OLIMPIO; GARCIA-CAIRASCO, NORBERTO; ALBERICI, LUCIANE CARLA. Energy Metabolism and Redox State in Brains of Wistar Audiogenic Rats, a Genetic Model of Epilepsy. FRONTIERS IN NEUROLOGY, v. 10, OCT 1 2019. Web of Science Citations: 0.
CUNHA, ALEXANDRA O. S.; DE DEUS, JUNIA L.; CEBALLOS, CESAR C.; LEAO, RICARDO M. Increased hippocampal GABAergic inhibition after long-term high-intensity sound exposure. PLoS One, v. 14, n. 5 MAY 8 2019. Web of Science Citations: 1.
SIQUEIRA CUNHA, ALEXANDRA OLIMPIO; CEBALLOS, CESAR CELIS; DE DEUS, JUNIA LARA; DE OLIVEIRA PENA, RODRIGO FELIPE; CORTES DE OLIVEIRA, JOSE ANTONIO; ROQUE, ANTONIO CARLOS; GARCIA-CAIRASCO, NORBERTO; LEAO, RICARDO MAURICIO. Intrinsic and synaptic properties of hippocampal CA1 pyramidal neurons of the Wistar Audiogenic Rat (WAR) strain, a genetic model of epilepsy. SCIENTIFIC REPORTS, v. 8, JUL 10 2018. Web of Science Citations: 2.
SIQUEIRA CUNHA, ALEXANDRA OLIMPIO; CEBALLOS, CESAR CELIS; DE DEUS, JUNIA LARA; LEAO, RICARDO MAURICIO. Long-term high-intensity sound stimulation inhibits h current (I-h) in CA1 pyramidal neurons. European Journal of Neuroscience, v. 47, n. 11, p. 1401-1413, JUN 2018. Web of Science Citations: 1.

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