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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intrinsic and synaptic properties of hippocampal CA1 pyramidal neurons of the Wistar Audiogenic Rat (WAR) strain, a genetic model of epilepsy

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Siqueira Cunha, Alexandra Olimpio [1] ; Ceballos, Cesar Celis [1, 2] ; de Deus, Junia Lara [1] ; de Oliveira Pena, Rodrigo Felipe [2] ; Cortes de Oliveira, Jose Antonio [1] ; Roque, Antonio Carlos [2] ; Garcia-Cairasco, Norberto [1] ; Leao, Ricardo Mauricio [1]
Total Authors: 8
[1] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Philosophy Sci & Letters Ribeirao Preto, Dept Phys, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 8, JUL 10 2018.
Web of Science Citations: 1

Despite the many studies focusing on epilepsy, a lot of the basic mechanisms underlying seizure susceptibility are mainly unclear. Here, we studied cellular electrical excitability, as well as excitatory and inhibitory synaptic neurotransmission of CA1 pyramidal neurons from the dorsal hippocampus of a genetic model of epilepsy, the Wistar Audiogenic Rat (WARs) in which limbic seizures appear after repeated audiogenic stimulation. We examined intrinsic properties of neurons, as well as EPSCs evoked by Schaffer-collateral stimulation in slices from WARs and Wistar parental strain. We also analyzed spontaneous IPSCs and quantal miniature inhibitory events. Our data show that even in the absence of previous seizures, GABAergic neurotransmission is reduced in the dorsal hippocampus of WARs. We observed a decrease in the frequency of IPSCs and mIPSCs. Moreover, mIPSCs of WARs had faster rise times, indicating that they probably arise from more proximal synapses. Finally, intrinsic membrane properties, firing and excitatory neurotransmission mediated by both NMDA and non-NMDA receptors are similar to the parental strain. Since GABAergic inhibition towards CA1 pyramidal neurons is reduced in WARs, the inhibitory network could be ineffective to prevent the seizure-dependent spread of hyperexcitation. These functional changes could make these animals more susceptible to the limbic seizures observed during the audiogenic kindling. (AU)

FAPESP's process: 15/22327-7 - Analysis of electrophysiological and morphofunctional properties of neurons in the central nucleus of inferior colliculus related to genesis and spreading of sound-induced convulsive seizures
Grantee:Alexandra Olimpio Siqueira Cunha
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 13/07699-0 - Research, Innovation and Dissemination Center for Neuromathematics - NeuroMat
Grantee:Jefferson Antonio Galves
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 15/50122-0 - Dynamic phenomena in complex networks: basics and applications
Grantee:Elbert Einstein Nehrer Macau
Support type: Research Projects - Thematic Grants
FAPESP's process: 16/01607-4 - High intensity sound stimulation and synaptic plasticity
Grantee:Ricardo Mauricio Xavier Leão
Support type: Regular Research Grants