Study of the effects of extracellular vesicles released by Leishmania amazonensis promastigotes on macrophages response and disease progression

• Grant number: 16/17245-4
• Support Opportunities: Regular Research Grants
• Duration: December 01, 2016 - November 30, 2018
• Field of knowledge: Health Sciences - Pharmacy
• Principal Investigator: Patricia Xander Batista
• Grantee: Patricia Xander Batista
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil
• Associated researchers: Wagner Luiz Batista
• Associated scholarship(s): 18/06597-2 - Study of the effects of extracellular vesicles released by Leishmania amazonensis promastigotes on macrophages response and disease progression, BP.TT

Abstract

Leishmania spp is the etiological agent of leishmaniasis a group of diseases with a broad spectrum of signs and symptoms ranging from cutaneous to visceral leishmaniasis. The disease is initiated by the interaction between the parasite molecules and receptors expressed in the host cell surface. In mammalian hosts, the parasite is able to infect and proliferate within phagocytic cells, especially macrophages. The parasite-host interaction is not fully understood and has recently been shown that extracellular vesicles (EVs) released by some species of Leishmania appear to contribute to the establishment of infection and immunomodulation. EVs secreted by parasites and micro-organisms appear to contribute to the pathogenesis of infectious diseases, but the mechanisms involved in this process have not been completely understood. Moreover, studies have not been performed to verify the role of EVs produced by L. amazonensis (specie responsible for cutaneous leishmaniasis in Brazil) in the activation and/or modulation of phagocytic cells of the immune system and disease progression. This project aim to evaluate the influence of EVs released by L. amazonensis promastigotes on immune response. This project can contribute to a better understanding of the host-parasite relationship and modulation/activation of the immune system in L. amazonensis infection. These results can be further used in the identification of new molecular targets as well as the development of alternative therapeutic and diagnostic strategies. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

COSTA BARBOSA, FERNANDA MARINS; DUPIN, TALITA VIEIRA; TOLEDO, MAYTE DOS SANTOS; DOS CAMPOS REIS, NATASHA FERRAZ; RIBEIRO, KLEBER; CRONEMBERGER-ANDRADE, ANDRE; RUGANI, JERONIMO NUNES; PIZARRO DE LORENZO, BEATRIZ HELENA; NOVAES E BRITO, RONNI ROMULO; SOARES, RODRIGO PEDRO; et al. Extracellular Vesicles Released by Leishmania (Leishmania) amazonensis Promote Disease Progression and Induce the Production of Different Cytokines in Macrophages and B-1 Cells. FRONTIERS IN MICROBIOLOGY, v. 9, DEC 21 2018. (16/17245-4)

NOVAES E BRITO, RONNI ROMULO; TOLEDO, MAYTE DOS SANTOS; LABUSSIERE, GABRIELA MARTINS; DUPIN, TALITA VIEIRA; DE CAMPOS REIS, NATASHA FERRAZ; PEREZ, ELIZABETH CRISTINA; XANDER, PATRICIA. B-1 cell response in immunity against parasites. Parasitology Research, v. 118, n. 5, p. 1343-1352, MAY 2019. (16/17245-4)

SOARES, RODRIGO P.; XANDER, PATRICIA; COSTA, ADRIANA OLIVEIRA; MARCILLA, ANTONIO; MENEZES-NETO, ARMANDO; DEL PORTILLO, HERNANDO; WITWER, KENNETH; WAUBEN, MARCA; NOLTE-'T HOEN, ESTHER; OLIVIER, MARTIN; et al. Highlights of the Sao Paulo ISEV workshop on extracellular vesicles in cross-kingdom communication. JOURNAL OF EXTRACELLULAR VESICLES, v. 6, n. 1, NOV 26 2017. (16/17245-4, 16/01917-3, 16/12111-0)

REIS, NATASHA FERRAZ DE CAMPOS; DUPIN, TALITA VIEIRA; COSTA, CAROLINA RIZZARO; TOLEDO, MAYTE DOS SANTOS; DE OLIVEIRA, VIVIAN CRISTINA; POPI, ANA FLAVIA; TORRECILHAS, ANA CLAUDIA; XANDER, PATRICIA. Leishmania amazonensis Promastigotes or Extracellular Vesicles Modulate B-1 Cell Activation and Differentiation. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 10, OCT 30 2020. (19/21614-3, 18/06597-2, 16/17245-4)