Profile of gene expression associated with heat stress in Nellore and Caracu

• Grant number: 16/19222-1
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: May 31, 2019
• Field of knowledge: Agronomical Sciences - Animal Husbandry - Genetics and Improvement of Domestic Animals
• Principal Investigator: Claudia Cristina Paro de Paz
• Grantee: Claudia Cristina Paro de Paz
• Host Institution: Instituto de Zootecnia. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). Nova Odessa , SP, Brazil
• Associated researchers: Danísio Prado Munari ; João Alberto Negrão ; Joslaine Noely dos Santos Goncalves Cyrillo ; Lenira El Faro Zadra ; Maria Eugênia Zerlotti Mercadante ; Maria Lucia Pereira Lima ; Nedenia Bonvino Stafuzza

Abstract

The primary purpose of this proposal is to quantify and compare the effect of thermal challenge on the expression profile of coding genes of the heat shock proteins 60, 70 and 90 (Hsp60, Hsp70 and Hsp90) among Nellore (Bos indicus) and Caracu (Bos taurus) breeds of cattle. During the summer months, when temperatures reach their peak (October-February), the rectal and skin surface temperatures, heart and respiratory rates will be measured in the animals of both races. Animal blood samples will be collected before (in the morning - 7:30 am) and after (the afternoon - 3:00 pm) exposure to the sun, for metering adrenocorticotropic hormone (ATCH) and cortisol. Blood samples will be used for mRNA extraction and study of the levels of transcripts of the coding genes of the heat shock proteins Hsp60, Hsp70 and Hsp90 by real-time PCR. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LIMA, SERGIO B. G. P. N. P.; STAFUZZA, NEDENIA BONVINO; PIRES, BIANCA V.; BONILHA, SARAH F. M.; CYRILLO, JOSLAINE N. S. G.; NEGRAO, JOAO A.; PAZ, CLAUDIA C. P.. Effect of high temperature on physiological parameters of Nelore (Bos taurus indicus) and Caracu (Bos taurus taurus) cattle breeds. TROPICAL ANIMAL HEALTH AND PRODUCTION, v. 52, n. 5, MAR 2020. (18/19216-7, 19/10438-0, 16/19222-1)

PIRES, B. V.; STAFUZZA, N. B.; LIMA, S. B. G. P. N. P.; NEGRAO, J. A.; PAZ, C. C. P.. Differential expression of heat shock protein genes associated with heat stress in Nelore and Caracu beef cattle. LIVESTOCK SCIENCE, v. 230, DEC 2019. (18/19216-7, 19/10438-0, 16/19222-1)

PIRES, BIANCA VILELA; FREITAS, ANA CLAUDIA; KLEIN, JOHN LENON; DE MELO, THAISE PINTO; STAFUZZA, NEDENIA BONVINO; PARO DE PAZ, CLAUDIA CRISTINA. Meta-analysis and meta-regression of core body temperature in taurine and zebuine cattle under different environmental conditions. LIVESTOCK SCIENCE, v. 265, p. 9-pg., 2022-10-28. (18/19216-7, 16/19222-1, 19/10438-0)

FREITAS, ANIELLY DE P.; LIMA, MARIA LUCIA P.; SIMILI, FLAVIA F.; NEGRAO, JOAO A.; SCHENKEL, FLAVIO S.; DE PAZ, CLAUDIA CRISTINA P.. Influence of handling in corrals on the temperament of different breeds of beef cattle raised in Brazil. JOURNAL OF ANIMAL SCIENCE, v. 101, p. 11-pg., 2023-01-03. (13/25355-6, 17/50339-5, 16/19222-1)

FREITAS, ANIELLY P.; LIMA, MARIA LUCIA P.; SIMILI, FLAVIA F.; SCHENKEL, FLAVIO S.; FARO, LENIRA E.; SANTANA, MARIO L.; PAZ, CLAUDIA CRISTINA P.. Genetic parameters for behavioral and growth traits of Nellore cattle. JOURNAL OF ANIMAL SCIENCE, v. 101, p. 10-pg., 2023-01-03. (17/50339-5, 16/19222-1)