The influence of commercially pure Titanium (cpTi) coating with Ca and P incorporation produced by plasma electrolytic oxidation on low density bone: An in vitro, ex-vivo and in vivo study

• Grant number: 16/20297-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: January 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Oral and Maxillofacial Surgery
• Principal Investigator: Leonardo Perez Faverani
• Grantee: Leonardo Perez Faverani
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated researchers: Nilson Cristino da Cruz ; Roberta Okamoto ; Valentim Adelino Ricardo Barão ; Wirley Goncalves Assuncao

Abstract

The medical and dental implants of titanium are frequently modified, especially its microstructure, obtaining better physical, chemical, and biological properties of the peri-implantar bone. Thus, the highest challenge current is to develop a surface on the topography of the implants to improve the bone healing on critical areas, mainly represented by bone with lower density. Therefore, this study will analyze the structural, molecular and healing parameters through a new coating method by plasma electrolytic oxidation (PEO) with Ca and P incorporation on the surface of the commercially pure titanium (cpTi) in low density bone. The structural characterization will be conducted on cpTi disks, which will be polished in a sequential granulation of sandpaper and then they will be submitted to the PEO method of Ca and P incorporation. The samples control will be treated with double etching-acid commercially available. After the texturing process, the disks will be analyzed in Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS), X-ray Difratography, Profilometry and Surface Free Energy (baseline). The animals used to the ex- in vivo and in vivo studies (Wistar female rats, n=10) will be divided into 3 groups: OBD Ovx (young rats - 6 months subjected to the ovariectomy for osteoporosis induction); ODN Sham (young rats - 6 months with normal bone density only induced at the same stress) and OBD Sen (old rats - 18 months with low bone density). The ex-vivo study will be conducted through culture of osteoblasts provided from calvaria bone of 60 female rats subjected to the same divisions as explained before (OBD Ovx, OBD Sen and ODN Sham). Thus, the total protein content, ALP activity and alizarin area will be analyzed. In the in vivo study, the same texturing methods performed on the disks will be carried out on the cpTi implants surface (2 x 4 mm) for placement in both tibias, for the following analyzes: reverse torque, PCR (OPG, RANKL, and OC), histological and histometric (Bone Implant Contact - BIC and Newly formed bone area - NB), microtomography (BV, BV/TV, Tb.Th, Tb.Sp, and Tb.N), of bone dynamics by fluorochromes (calcein/alizarin), and immunohistochemistry (OPG, RANKL, and OC). All quantitative data will be submitted to the normality curve to establish the best statistical test (parametric or nonparametric), considering P<0.05 significance level. Key-words: Osteoporosis, bone regeneration, Cell Culture Techniques, dental implants. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FAVERANI, LEONARDO P.; SILVA, WILLIAM P. P.; DE SOUSA, CECILIA ALVES; FREITAS, GILEADE; BASSI, ANA PAULA F.; SHIBLI, JAMIL A.; BARAO, VALENTIM A. R.; ROSA, ADALBERTO L.; SUKOTJO, CORTINO; ASSUNCAO, WIRLEY G.. apping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coatin. MATERIALS, v. 15, n. 3, FEB 2022. (16/20297-6)

BRAGA POLO, TARIK OKON; DA SILVA, WILLIAM PHILLIP; CORREA MOMESSO, GUSTAVO ANTONIO; LIMA-NETO, TIBURTINO JOSE; BARBOSA, STEFANY; CORDEIRO, JAIRO MATOZINHO; HASSUMI, JAQUELINE SUEMI; DA CRUZ, NILSON CRISTINO; OKAMOTO, ROBERTA; BARAO, VALENTIM A. R.; et al. Plasma Electrolytic Oxidation as a Feasible Surface Treatment for Biomedical Applications: an in vivo study. SCIENTIFIC REPORTS, v. 10, n. 1, JUN 19 2020. (16/20297-6)

BRAGA POLO, TARIK OCON; CORREA MOMESSO, GUSTAVO ANTONIO; PEREIRA SILVA, WILLIAM PHILLIP; DE SOUZA SANTOS, ANDERSON MAIKON; FONSECA-SANTOS, JOAO MATHEUS; DA CRUZ, NILSON CRISTINO; BARAO, VALENTIM A. R.; GARCIA, VALDIR GOUVEIA; THEODORO, LETICIA HELENA; FAVERANI, LEONARDO P.. Is an anodizing coating associated to the photobiomodulation able to optimize bone healing in ovariectomized animal model. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 217, APR 2021. (18/11496-0, 16/20297-6)

WILLIAM PHILLIP PEREIRA DA SILVA; LEONARDO ALAN DELANORA; BARBARA RIBEIRO RIOS; STÉFANY BARBOSA; MARIA ELOISE DE SÁ SIMON; CORTINO SUKOTJO; LEONARDO P FAVERANI. Feasible low bone density condition for assessing bioactivity in ex-in vivo and in vivo studies. Journal of Applied Oral Science, v. 31, 2023-07-07. (16/20297-6)

CORREA MOMESSO, GUSTAVO ANTONIO; BRAGA POLO, TARIK OCON; PEREIRA DA SILVA, WILLIAM PHILLIP; BARBOSA, STEFANY; FREITAS, GILEADE P.; LOPES, HELENA BACHA; ROSA, ADALBERTO LUIZ; CORDEIRO, JAIRO MATOZINHO; TORO, LUAN FELIPE; CHIBA, FERNANDO YAMAMOTO; et al. Miniplates coated by plasma electrolytic oxidation improve bone healing of simulated femoral fractures on low bone mineral density rats. Materials Science & Engineering C-Materials for Biological Applications, v. 120, JAN 2021. (16/20297-6, 18/00522-0)

POLO, TARIK OCON BRAGA; FONSECA-SANTOS, JOAO MATHEUS; MOMESSO, GUSTAVO ANTONIO CORREA; DA SILVA, WILLIAM PHILLIP PEREIRA; BARBOSA, STEFANY; SANTOS, ANDERSON MAIKON DE SOUZA; SILVA, MIRELA CAROLINE; GARCIA, VALDIR GOUVEIA; THEODORO, LETICIA HELENA; FAVERANI, LEONARDO P.. Single intraoperative infrared laser optimized bone repair in rat femoral osteotomies with experimentally induced osteoporosis. Lasers in Medical Science, v. 38, n. 1, p. 10-pg., 2023-03-20. (16/20297-6, 18/11496-0)

POLO, TARIK OCON BRAGA; SILVA, WILLIAM PHILLIP PEREIRA; MOMESSO, GUSTAVO ANTONIO CORREA; LIMA-NETO, TIBURTINO JOSE; BARBOSA, STEFANY; CORDEIRO, JAIRO MATOZINHO; HASSUMI, JAQUELINE SUEMI; DA CRUZ, NILSON CRISTINO; OKAMOTO, ROBERTA; BARAO, VALENTIM A. R.; et al. Plasma Electrolytic Oxidation as a Feasible Surface Treatment for Biomedical Applications: an in vivo study (vol 10, 10000, 2020). SCIENTIFIC REPORTS, v. 10, n. 1, p. 2-pg., 2020-10-28. (16/20297-6)

CORREIA MOMESSO, GUSTAVO ANTONIO; DE SOUZA SANTOS, ANDERSON MAIKON; FONSECA E SANTOS, JOAO MATHEUS; DA CRUZ, NILSON CRISTINO; OKAMOTO, ROBERTA; RICARDO BARAO, VALENTIM ADELINO; SIROMA, RAFAEL SHINOSKE; SHIBLI, JAMIL AWAD; FAVERANI, LEONARDO PEREZ. Comparison between Plasma Electrolytic Oxidation Coating and Sandblasted Acid-Etched Surface Treatment: Histometric, Tomographic, and Expression Levels of Osteoclastogenic Factors in Osteoporotic Rats. MATERIALS, v. 13, n. 7, APR 2020. (16/20297-6)