Physiological mechanisms involved in skin wound healing after treatment with menthol-based cream in diabetic and non-diabetic rats

• Grant number: 16/13307-5
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2017
• End date: February 28, 2019
• Field of knowledge: Biological Sciences - Physiology - Physiology of Organs and Systems
• Principal Investigator: Ariane Leite Rozza
• Grantee: Ariane Leite Rozza
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Claudia Helena Pellizzon

Abstract

The loss of skin integrity due to injuries results in the formation of a wound. In consequence, the healing process is triggered, reestablishing the function and integrity of the injured skin. The healing process is classified into three overlapping phases: inflammatory, proliferative and extracellular matrix remodeling phase. Menthol is the main compound of essential oils from genus Mentha, presenting antinociceptive, local anesthetic and antimicrobial effects, and has been related to glycemic decreasing and diabetes prevention. This research project aims at clarifying the physiological mechanisms triggered by menthol in skin wound healing, in diabetic and non-diabetic rats. After anesthesia, a circular excision of 2 cm of diameter will be created on the dorsal region of male Wistar rats. Rats will be distributed into 6 groups: base cream, collagenase cream, menthol-based cream at 0.25, 0.5 and 1.0% and sham group. The wounds will be treated and measured daily for 3, 7 or 14 days (according to the phases described in the literature). Diabetes will be streptozotocin-induced in another group of rats, in which the skin wound will be developed and treated with base cream, insulin-based cream or menthol-based cream (lower effective concentration) during 14 days. After euthanasia, histological, histomorphometrical and immunohistochemical analyses will be performed, besides antioxidant and inflammatory activity analyzes, metalloproteinase activity and protein and gene expression. Analyses of hepatic and renal dysfunction will be performed in the rats' serum, besides histopathological analyzes in rat organs. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROZZA, ARIANE LEITE; BESERRA, FERNANDO PEREIRA; VIEIRA, ANA JULIA; OLIVEIRA DE SOUZA, EDUARDO; HUSSNI, CARLOS ALBERTO; MARTINEZ, EMANUEL RICARDO MONTEIRO; NOBREGA, RAFAEL HENRIQUE; PELLIZZON, CLAUDIA HELENA. The Use of Menthol in Skin Wound Healing-Anti-Inflammatory Potential, Antioxidant Defense System Stimulation and Increased Epithelialization. PHARMACEUTICS, v. 13, n. 11, NOV 2021. (13/23340-1, 13/22202-4, 15/06636-0, 16/13307-5)