| Grant number: | 16/20177-0 |
| Support Opportunities: | Regular Research Grants |
| Start date: | May 01, 2017 |
| End date: | April 30, 2019 |
| Field of knowledge: | Biological Sciences - Zoology - Morphology of Recent Groups |
| Principal Investigator: | Antonio Leão Castilho |
| Grantee: | Antonio Leão Castilho |
| Host Institution: | Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil |
| Associated researchers: | Fernando Jose Zara ; George Shigueki Yasui ; Graziela Gorete Romagnoli ; Sérgio Luiz de Siqueira Bueno ; Vladimir Eliodoro Costa |
| Associated scholarship(s): | 18/04740-2 - Sampling and analysis of Aegla castro Schmitt, 1942, BP.TT |
Abstract
Anomurans Aegla spp. are endemics of South American Neotropical fauna and are fragile in environmental disturbance so much that there are species in extinction dangerous. Aegla castro has a geographical distribution of southern region of São Paulo state until northern Parana state, a wide distribution that could represent a complex of cryptic species. The aim of this project is to study individual of A. castro in different locations in order to verify if this taxon corresponds a complex of cryptic species. The animals will be collected with passive (trap) and active (net) methodologies and will be investigated the external morphology and dissected to the spermatozoal ultrastructure analysis (spermeotaxonomy). The nuclear DNA analysis will be conducted according the flow cytometer methodology that measure the DNA fluorescence intensity of the muscle (claw), identified with fluorescence marker. Spermeotaxonomy and nuclear DNA of two external groups, Aegla parana and A. marginata, will be analyzed with the aim to compare if the intraspecific morphologic and genetic range (DNA fluorescence intensity) are higher than interspecific ones. We propose that the genetic isolation range correspond an inverse relation with migratory ability or direct relation with geographic isolation. Stable isotope is an efficient tool with low costs and high precision to measure migration route that we will measure the Carbon, Hydrogen, and Nitrogen isotopes of the claw muscle. They will be compared with local isotopes of diet stomach content and the river water. With morphology, genetic and stable isotope focus we will test our hypothesis that there is not morphological difference, but genetic ones, along the A. castro geographic distribution, justified by the particular isotopic signature of each animal group according to the local habitat signature. (AU)
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