Clinical and microbial evaluation of subjects under Periodontal Maintenace Therapy: A Prospective Cohort Study.

• Grant number: 16/15999-1
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2017
• End date: July 31, 2019
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: José Roberto Cortelli
• Grantee: José Roberto Cortelli
• Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade de Taubaté (UNITAU). Taubaté , SP, Brazil
• Associated researchers: Fernando de Oliveira Costa ; Sheila Cavalca Cortelli

Abstract

The present prospective open cohort study of 6 years of follow-up within a periodontal maintenance therapy program will evaluate in 56 subjects paired by gender (50% females) and age the levels of A. actinomycetemcomitans (A); the occurrence and distribution of a-b-c-d-e-f-g serotypes (B); the prevalence and distribution of A. actinomycetemcomitans JP2 and Non JP2 clones (C); detection of the cytolethal distending toxin genes (CdtA-CdtB and CdtC) (D); and the relationship between clinical and microbial parameters (E). The sample population will be composed of 28 regular compliers (mean age 54.6± 7.5 years) and 28 irregular compliers (mean age 52.5± 7.3 years). Therefore, it will be compared the effects of periodontal maintenance therapy between regular and irregular compliers on periodontal stability and it will be determined the relation among periodontal clinical parameters (periodontal pocket depth, clinical attachment level, blending on probing, furcation involvement, periodontal suppuration, plaque index) presence, levels and virulence of A. actinomycetemcomitans. Finally, it will be also compared results from both molecular techniques - conventional and real time polymerase chain reaction techniques (PCR and RT-PCR, respectively) to detect A. actinomycetemcomitans at baseline (at the end of active periodontal therapy) and at 6 years of periodontal maintenance therapy. The clinical periodontal examinations were performed by trained and systematically calibrated periodontists along the periodontal maintenance visits (mean 8.7± 4.0). Subgingival biofilm samples were collected, once a year, through standard procedures from the deepest periodontal pockets and storage at -80oC for further laboratorial processing. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORTELLI, JOSE ROBERTO; CORTELLI, SHEILA CAVALCA; AQUINO, DAVI ROMEIRO; MIRANDA, TAIS BROWNE; MOREIRA JARDIM, JOAO CARLOS; COSTA, FERNANDO DE OLIVEIRA. Aggregatibacter actinomycetemcomitans serotypes and JP2 outcomes related to clinical status over 6 years under periodontal maintenance therapy. ARCHIVES OF ORAL BIOLOGY, v. 116, AUG 2020. (16/15999-1)