Periodontal plastic surgery: assessment of periodontal wound healing immunology and randomized clinical trial for the development of new treatment protocol

• Grant number: 16/16102-5
• Support Opportunities: Regular Research Grants
• Start date: May 01, 2017
• End date: June 30, 2019
• Field of knowledge: Health Sciences - Dentistry - Periodontology
• Principal Investigator: Mauro Pedrine Santamaria
• Grantee: Mauro Pedrine Santamaria
• Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Gingival recession is a very common problem in the dental clinic. It can affect up to 100% of the population and can bring some negative clinical conditions such as dentin sensitivity and esthetic problems. As a result, many surgical techniques were developed to deal with this problem. However, even the best surgical techniques present a great variability in their results, and not all patients feel completely satisfied with the final esthetic outcome. One of the reasons that may explain this variation is the differences in the wound healing process of each patient. Most information on soft tissue healing comes from studies with epidermal, also termed cutaneous or skin wound healing; much less is known about the cellular aspects of soft tissue healing in the oral mucosa. Understanding wound healing at the molecular level provides the scientific basis to develop targeted strategies, mainly with the intension to overcome delayed wound healing or improve the results of periodontal plastic surgeries. Additionally, known methods to increase the healing speed of some medical conditions are barely used in periodontal plastic surgery. The understanding of their effects on clinical outcomes and on the molecular level of immunological biomarkers during gingival wound healing may set new treatment protocols in the periodontal plastic surgery armamentarium. Thus, the aim of the present study is to evaluate concentrations of inflammatory mediators and growth factors during the healing period of periodontal plastic surgery. Additionally, we aim to evaluate different methods of tissue biostimulation on periodontal wound healing after periodontal plastic surgery and their effects on the concentrations of inflammatory mediators and growth factors during the healing period. For this study will have 2 parts: 1. Randomized clinical trial in which it will evaluate the clinical and the immunological outcomes of a electrial stimulation protocol as adjunctive to coronally advanced flap for the treatment of gingival recession. The concentration of the immunological targets will be correlated with the clinical parameters in order to determine whether citokines or growth factors play a role on the clinical outcomes. 2. A mouse study that aims to evaluate whether the electrical stimulation protocol can speed the wound closure on oral palate mucosa e how this stimulation can change the concentration of each inflammatory and growth factor targets. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, CAMILA LOPES; NEVES JARDINI, MARIA APARECIDA; MORETTO NUNES, CAMILLA MAGNONI; BERNARDO, DANIELLA VICENSOTTO; VIANA CASARIN, RENATO CORREA; GEDRAITE, ESTEVAO DOS SANTOS; MATHIAS, MARCIO ANTONIO; LIU, FEI; MENDONCA, GUSTAVO; SILVEIRA MENDONCA, DANIELA BACCELLI; et al. Electrical stimulation enhances early palatal wound healing in mice. ARCHIVES OF ORAL BIOLOGY, v. 122, FEB 2021. (16/07528-9, 16/16102-5)

VIANA MIGUEL, MANUELA MARIA; MATHIAS-SANTAMARIA, INGRID FERNANDES; ROSSATO, AMANDA; FERREIRA FERRAZ, LAIS FERNANDA; FIGUEIREDO-NETO, ANTONIO MARTINS; DE MARCO, ANDREA CARVALHO; VIANA CASARIN, RENATO CORREA; WALLET, SHANNON MARGARET; TATAKIS, DIMITRIS N.; MATHIAS, MARCIO ANTONIO; et al. Microcurrent electrotherapy improves palatal wound healing: Randomized clinical trial. Journal of Periodontology, v. 92, n. 2, AUG 2020. (18/03353-5, 18/07340-5, 16/16102-5, 16/07528-9, 16/24531-3, 14/50983-3)