Mercury toxicity biomarker characterization in fish from Basin of the Madeira River-Rondônia using metalloproteomic strategies

• Grant number: 16/19404-2
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2017
• End date: May 31, 2019
• Field of knowledge: Biological Sciences - Biochemistry - Chemistry of Macromolecules
• Principal Investigator: Pedro de Magalhães Padilha
• Grantee: Pedro de Magalhães Padilha
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Camila Pereira Braga ; Jiri Adamec ; Marcelo de Oliveira Lima ; Marília Afonso Rabelo Buzalaf

Abstract

In recent decades, high mercury concentrations have been found in soils, sediments, fish and humans in the Amazon. However, studies evaluating mercury toxicity mechanisms at the cellular level in fish and riverine populations are scarce. However, evaluating potential biomarkers related toxicity by mapping the expression of proteins associated with mercury may indicate fish contamination levels and subsequent human exposure. In this context, a metalloproteomic approach, a relatively new field, can be used to identify biomarkers associated with mercury proteins, thus contributing to the elucidation of physiological and functional aspects of mercury transport in the biota. Recent metalloproteomic studies of fish in the Amazon region, developed by researchers from UNESP Botucatu, allowed fractionation and characterisation of mercury-associated proteins which show biomarker characteristics. However, the physiological and functional aspects of these proteins associated with mercury in Amazonian fish and the adaptation mechanisms presented by these animals in relation to exposure to the metal have not yet been elucidated. The aim of this work is develop and validate analytical methods to advance the knowledge of protein/enzymatic biomarkers of mercury contamination in samples of plasma, muscle and hepatic tissues of fish collected in areas near the reservoir of the JIRAU Hydroelectric Power Plant - Madeira River Basin using the following metalloproteomic strategies: proteome fractionation of samples by 2D PAGE; mercury mapping in protein spots by GFAAS; characterization of proteins associated with mercury by ESI-MS-MS; evaluation of the oxidative stress of fish contaminated with mercury through the activities of the enzymes involved in antioxidant defense; bioinformatic procedures that may contribute to the knowledge of these proteins function, metabolic pathways analysis, determination of protein-protein interactions, and finally, development of a website to make the characterized mercury biomarkers available. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAVECCI-MENDONCA, BRUNA; DE SOUZA VIEIRA, JOSE CAVALCANTE; DE LIMA, PAULA MONTEIRO; LEITE, ALINE LIMA; RABELO BUZALAF, MARILIA AFONSO; ZARA, LUIZ FABRICIO; PADILHA, PEDRO DE MAGALHAES. Study of proteins with mercury in fish from the Amazon region. Food Chemistry, v. 309, MAR 20 2020. (14/02638-5, 16/19404-2, 13/21297-1)

SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; DE QUEIROZ, JOAO VITOR; CAVECCI-MENDONCA, BRUNA; DE OLIVEIRA, GRASIELI; DE FREITAS, NATALIA GALVAO; HENRIQUE FERNANDES, ANA ANGELICA; FERNANDES, MILENI DA SILVA; BUZALAF, MARILIA AFONSO RABELO; ADAMEC, JIRI; et al. The effects of mercury exposure on Amazonian fishes: An investigation of potential biomarkers. Chemosphere, v. 316, p. 13-pg., 2023-01-13. (16/19404-2, 19/02538-4, 14/02668-1)

BITTARELLO, ALIS CORREIA; SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; DE PAULA ARAUJO, WELLINGTON LUIZ; BATAGLIOLI, IZABELA DA CUNHA; DA SILVA, JANAINA MACEDO; RABELO BUZALAF, MARILIA AFONSO; FLEURI, LUCIANA FRANCISCO; PADILHA, PEDRO DE MAGALHAES. Characterization of molecular biomarkers of mercury exposure to muscle tissue of Plagioscion squamosissimus and Colossoma macropomum from the Amazon region. Food Chemistry, v. 276, p. 247-254, MAR 15 2019. (16/19404-2, 14/02668-1, 13/21297-1)

SOUZA VIEIRA, JOSE CAVALCANTE; DE OLIVEIRA, GRASIELI; CAVALLINI, NUBYA GONCALVES; BRAGA, CAMILA PEREIRA; ADAMEC, JIRI; ZARA, LUIZ FABRICIO; RABELO BUZALAF, MARILIA AFONSO; PADILHA, PEDRO DE MAGALHAES. nvestigation of Protein Biomarkers and Oxidative Stress in Pinirampus pirinampu Exposed to Mercury Species from the Madeira River, Amazon-Brazi. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 200, n. 4, SEP 2021. (16/19404-2, 14/02668-1)

BITTARELLO, ALIS CORREIA; SOUZA VIEIRA, JOSE CAVALCANTE; BRAGA, CAMILA PEREIRA; BATAGLIOLI, IZABELA DA CUNHA; DE OLIVEIRA, GRASIELI; ROCHA, LEONE CAMPOS; ZARA, LUIZ FABRICIO; RABELO BUZALAF, MARILIA AFONSO; SILVA DE OLIVEIRA, LINCOLN CARLOS; ADAMEC, JIRI; et al. Metalloproteomic approach of mercury -binding proteins in liver and kidney tissues of Plagioscion squamosissimus (corvina) and Colossoma macropomum (tambaqui) from Amazon region: Possible identification of mercury contamination biomarkers. Science of The Total Environment, v. 711, APR 1 2020. (14/02668-1, 16/19404-2)

BATAGLIOLI, IZABELA DA CUNHA; DE QUEIROZ, JOAO VITOR; SOUZA VIEIRA, JOSE CAVALCANTE; CAVALLINE, NUBYA GONCALVES; BRAGA, CAMILA PEREIRA; RABELO BUZALAF, MARILIA AFONSO; ZARA, LUIS FABRICIO; ADAMEC, JIRI; PADILHA, PEDRO DE MAGALHAES. Mercury metalloproteomic profile in muscle tissue of Arapaima gigas from the Brazilian Amazon. ENVIRONMENTAL MONITORING AND ASSESSMENT, v. 194, n. 10, p. 11-pg., 2022-10-01. (17/09466-3, 16/19404-2)

BATAGLIOLI, IZABELA DA CUNHA; SOUZA VIEIRA, JOSE CAVALCANTE; DE QUEIROZ, JOAO VITOR; FERNANDES, MILENI DA SILVA; BITTARELLO, ALIS CORREIA; BRAGA, CAMILA PEREIRA; RABELO BUZALAF, MARILIA AFONSO; ADAMEC, JIRI; ZARA, LUIZ FABRICIO; PADILHA, PEDRO DE MAGALHAES. Physiological and functional aspects of metal -binding protein associated with mercury in the liver tissue of pirarucu (Arapaima gigas) from the Brazilian Amazon. Chemosphere, v. 236, DEC 2019. (17/09466-3, 16/19404-2)

DE QUEIROZ, JOAO VITOR; CAVECCI-MENDONCA, BRUNA; SOUZA VIEIRA, JOSE CAVALCANTE; MARTINS, RENATA APARECIDA; DE ALMEIDA ASSUNCAO, ANDREY SAVIO; CAVALLINI, NUBYA GONCALVES; DOS SANTOS, FELIPE ANDRE; PADILHA, PEDRO DE MAGALHAES. Metalloproteomic Strategies for Identifying Proteins as Biomarkers of Mercury Exposure in Serrasalmus rhombeus from the Amazon Region. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 199, n. 2, MAY 2020. (13/21297-1, 16/19404-2)

DE QUEIROZ, JOAO VITOR; SOUZA VIEIRA, JOSE CAVALCANTE; DE OLIVEIRA, GRASIELI; BRAGA, CAMILA PEREIRA; BATAGLIOLI, IZABELA DA CUNHA; DA SILVA, JANAINA MACEDO; DE PAULA ARAUJO, WELLINGTON LUIZ; PADILHA, PEDRO DE MAGALHAES. Identification of Biomarkers of Mercury Contamination in Brachyplatystoma filamentosum of the Madeira River, Brazil, Using Metalloproteomic Strategies. BIOLOGICAL TRACE ELEMENT RESEARCH, v. 187, n. 1, p. 291-300, JAN 2019. (13/21297-1, 16/19404-2)