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Hydrolysis of cassava bagasse assisted by high-intensity ultrasound: acidic saccharification of residual starch with posterior enzymatic treatment of lignocellulosic biomass


The increasing interest by non-conventional techniques has motivated new researches based on pretreatment of different residues from agroindustry to make viable bioethanol production. Power ultrasound, or high-intensity ultrasound, has been highlighted due to the cavitation effects on materials structure, for example. In combination with the availability of residues from cassava starch extraction processing, the aim of this project is to study two processes assisted by high-intensity ultrasound: 1. Acid hydrolysis of cassava bagasse to digest residual starch and 2. Enzymatic hydrolysis of the fibrous residue from first step. For this, the bagasse will be characterized according to starch, cellulose, hemicellulose and lignin content, whereas the different acid suspensions with cassava bagasse will had their density, rheological behavior, specific heat and vapor pressure determined and modeled. Structure effects will be evaluated through scanning electronic microscopy, changes in particle size and crystalinity index. In the best and worst conditions of the two steps, a sugar and undesirable compounds profile will be obtained by high performance liquid cromatography. Real powers and intensities will be determined and mapped in different points of the vessel with the aid of hydrophones and the calorimetric method. Thus, it is expected a well-designed sonication process that performs the saccharification of residual starch, increases the acessibility of lignocellulose for releasing sugar and enables bioethanol production. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
POLACHINI, TIAGO CARREGARI; HERNANDO, ISABEL; MULET, ANTONIO; TELIS-ROMERO, JAVIER; CARCEL, JUAN A. Ultrasound-assisted acid hydrolysis of cassava (Manihot esculenta) bagasse: Kinetics, acoustic field and structural effects. ULTRASONICS SONOCHEMISTRY, v. 70, JAN 2021. Web of Science Citations: 0.
POLACHINI, TIAGO CARREGARI; MULET, ANTONIO; CARCEL, JUAN A.; TELIS-ROMERO, JAVIER. Thermophysical properties of dilute acid slurries of cassava bagasse as a function of biomass loading, acid concentration, and temperature. ENVIRONMENTAL PROGRESS & SUSTAINABLE ENERGY, OCT 2020. Web of Science Citations: 0.
CARREGARI POLACHINI, TIAGO; MULET, ANTONIO; TELIS-ROMERO, JAVIER; CARCEL, JUAN A. Influence of high-intensity ultrasound application on the kinetics of sugar release from acid suspensions of artichoke (Cynara scolymus) biomass. CHEMICAL ENGINEERING AND PROCESSING, v. 145, NOV 2019. Web of Science Citations: 0.
CARREGARI POLACHINI, TIAGO; MULET, ANTONIO; CARCEL, JUAN A.; TELIS-ROMERO, JAVIER. Rheology of acid suspensions containing cassava bagasse: Effect of biomass loading, acid content and temperature. Powder Technology, v. 354, p. 271-280, SEP 2019. Web of Science Citations: 0.
DE CARVALHO, GISANDRO REIS; POLACHINI, TIAGO CARREGARI; DARROS-BARBOSA, ROGER; BON, JOSE; TELIS-ROMERO, JAVIER. Effect of intermittent high-intensity sonication and temperature on barley steeping for malt production. JOURNAL OF CEREAL SCIENCE, v. 82, p. 138-145, JUL 2018. Web of Science Citations: 2.

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