Development of lignin active enzyme mixture to produce sugars from lignocellulosic biomass for production of fuels, chemicals and bio based materials

• Grant number: 17/50025-0
• Support Opportunities: Regular Research Grants
• Start date: July 01, 2017
• End date: June 30, 2019
• Field of knowledge: Interdisciplinary Subjects
• Agreement: University of Nebraska - Lincoln
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Fernando Segato
• Grantee: Fernando Segato
• Principal researcher abroad: Mark Wilkins
• Institution abroad: University of Nebraska-Lincoln (UNL), United States
• Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil
• Associated research grant: 14/18714-2 - Enzymatic oxidation of sugarcane bagasse: discovery, characterization and new application of oxidative enzymes active in carbohydrates, applied to the enhancement of a fungal cell factory, AP.BIOEN.JP

Abstract

Production of fuels and chemicals from renewable lignocellulosic biomass is difficult due to its recalcitrance to deconstruction. This recalcitrance is mostly due to presence of lignin in plant cell walls. Lignin is disrupted and/or removed from plant cell walls through expensive physicochemical pretreatments that use harsh chemicals, high temperatures and high pressures. Lignin in lignocellulosic biomass is resistant to attack by most organisms; however, white rot fungi produce a number of enzymes that can degrade lignin. This project would combine the talents of two researchers, Dr. Mark Wilkins of the University of Nebraska-Lincoln and Dr. Femando Segato of the University of Sao Paulo, to develop an enzyme cocktail that degrades lignin, cellulose and hemicellulose to produce sugars that can be fermented by microbes to produce fuels and chemicals. The cocktail would indude lignin-degrading enzymes from Myceliopthora thermophila that have been heterologously expressed by the Segato lab. Sugarcane bagasse and com stover would be pretreated using liquid, hot water pretreatment with various degrees of severity in Wilkins lab, and then this biomass would be hydrolyzed using various combinations of lignin- degrading enzymes and cellulase. A response surface design would be used to optimize the enzyme cocktail. Each PD will visit each other's institution twice. In the first year, the main lab experiments will be conducted and in the second year each PD will train the other PD in their area of specialization. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CAMARGO, SUELEN; MULINARI, EVANDRO J.; DE ALMEIDA, LEONARDO R.; BERNARDES, AMANDA; PRADE, ROLF A.; GARCIA, WANIUS; SEGATO, FERNANDO; MUNIZ, JOAO R. C.. Functional and structural characterization of an alpha-L-arabinofuranosidase from Thermothielavioides terrestris and its exquisite domain-swapped beta-propeller fold crystal packing. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, v. 1868, n. 12, p. 12-pg., 2020-12-01. (17/16291-5, 16/09152-6, 14/18714-2, 14/50897-0, 19/22284-7, 17/50025-0)

LIU, ENSHI; SEGATO, FERNANDO; PRADE, ROLF A.; WILKINS, MARK R.. Exploring lignin depolymerization by a bi-enzyme system containing aryl alcohol oxidase and lignin peroxidase in aqueous biocompatible ionic liquids. APPLIED GEOCHEMISTRY, v. 131, AUG 2021. (14/18714-2, 17/50025-0)

VELASCO, JOSMAN; PELLEGRINI, VANESSA DE OLIVEIRA ARNOLDI; SEPULCHRO, ANA GABRIELA VEIGA; KADOWAKI, MARCO ANTONIO SEIKI; SANTO, MELISSA CRISTINA ESPIRITO; POLIKARPOV, IGOR; SEGATO, FERNANDO. Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification. Enzyme and Microbial Technology, v. 144, MAR 2021. (19/22284-7, 15/13684-0, 14/06923-6, 14/18714-2, 19/01165-0, 17/50025-0)

LIU, ENSHI; SEGATO, FERNANDO; WILKINS, MARK R.. Fed-batch production of Thermothelomyces thermophilus lignin peroxidase using a recombinant Aspergillus nidulans strain in stirred-tank bioreactor. Bioresource Technology, v. 325, APR 2021. (14/18714-2, 17/50025-0)

LIU, ENSHI; SEGATO, FERNANDO; PRADE, ROLF A.; WILKINS, MARK R.. xploring lignin depolymerization by a bi-enzyme system containing aryl alcohol oxidase and lignin peroxidase in aqueous biocompatible ionic liquid. Bioresource Technology, v. 338, OCT 2021. (14/18714-2, 17/50025-0)