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Interactions between inflammation and obesity: mechanisms by which the inflammatory process affects adipogenesis and obesity complications

Grant number: 17/05232-8
Support type:Regular Research Grants
Duration: September 01, 2017 - February 28, 2019
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal researcher:Heraldo Possolo de Souza
Grantee:Heraldo Possolo de Souza
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers: Mauro Figueiredo Carvalho de Andrade ; Thais Martins de Lima Salgado

Abstract

There is evidence that obesity complications such as type 2 diabetes and dyslipidemia can be attributed, at least partially, to the inflammatory response that occurs within adipose tissue.However, it is still unclear whether inflammation alone may play a role in the genesis of obesity. We also do not know the influence of an acute inflammatory event, such as that observed in sepsis, on energy metabolism and the development of obesity complications. So we designed two subprojects to try to answer these questions. In subproject # 1 the objective is to determine the molecular and cellular mechanisms by which lymphatic obstruction leads to the formation of adipose tissue in mouse tails. An experimental model of adipogenesis in mice will be used, where the lymphatic vessels of the tail will be ligated. To determine the mechanisms by which lymphatic stasis induces the formation of adipose tissue, the concentrations of adipokines, inflammatory cytokines, nuclear receptors (PPARs, CREB, etc.), growth factors will be determined, both by quantitative PCR and by immunohistochemistry. To establish a causal relationship between inflammatory mediators and adipogenesis, inflammatory pathways will be pharmacologically blocked or stimulated and transgenic mice will be used.In subproject # 2, animals rendered obese by increased caloric intake will be subjected to severe inflammatory aggression, such as that caused by sepsis. Survivors will be evaluated for their metabolic parameters and the characteristics of the inflammatory infiltrate present in adipose tissue.Both subprojects have the potential to generate translational knowledge relevant both to a better understanding of those diseases that develop through the deregulation of metabolic signaling pathways, inflammation and proliferation, and to new therapeutic targets for these diseases. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CUADRADO, GUILHERME A.; DE ANDRADE, MAURO F. C.; ARIGA, SUELY K.; DE LIMA, THAIS M.; SOUZA, HERALDO P. Inflammation Precedes Fat Deposition in an Experimental Model of Lymphedema. LYMPHATIC RESEARCH AND BIOLOGY, NOV 2020. Web of Science Citations: 0.
ARRUDA, G.; ARIGA, S.; DE LIMA, T. M.; SOUZA, H. P.; ANDRADE, M. A MODIFIED MOUSE-TAIL LYMPHEDEMA MODEL. LYMPHOLOGY, v. 53, n. 1, p. 29-37, MAR 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.