Development of alternative methods for fabrication of electrochemical paper-based sensors

• Grant number: 17/10522-5
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2017
• End date: September 30, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Thiago Regis Longo Cesar da Paixão
• Grantee: Thiago Regis Longo Cesar da Paixão
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Paper has been used as substrate to perform analytical tests for centuries however, since 2007 microfluidically patterned paper substrates opened up a large new area of sensing applications that have previously exclusively been available to microfluidic devices made from glass or synthetic polymeric materials. Based on the paper characteristics (liquid through capillary action, the capability of modification and reagents storage inside the fiber network, availability and large-scale production), this material is suitable for analytical devices fabrication. The microfluidic paper-based analytical device (µPADs) enabled to develop low cost, disposable and ecofriendly devices, that allows to perform analysis in developing countries, low-resource areas and point-of-care where lacking infrastructure and trained professionals. However, since colorimetric methods have been used preferentially for detection, the range of application of these devices it is not wide as it could be. Real samples have complex composition, hence the presence of species with similar chemical characteristics can hinder or even become impossible to qualify or quantify the analyte without of more selectivity and sensitivity analytical methods. In this sense, electrochemical detection provides a quantitative and alternative detection system for ¼PADs and can improve the performance of ¼PADs in terms of sensitivity and selectivity. Paper-based electrochemical devices (ePADs) were first published in 2009 and since then different fabrication methods have been reported using chemical reagent deposition and chemical atmosphere control for the manufacture of ePADs . In this project, we will try to fabricate ePADs devices without the need of chemical reagents using a laser to perform the paper pyrolysis creating devices without the need of chemical reagents and with low cost. In addition, alternative methods with the deposition of nanomaterials and metallic low cost materials are also reported for the development of electrochemical sensors in the forensic, clinical and environmental area. (AU)

Scientific publications

(26)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MENDES, LETICIA FRANCINE; SOUZA E SILVA, ANGELA RODRIGUES; BACIL, RAPHAEL PRATA; PIRES SERRANO, SILVIA HELENA; ANGNES, LUCIO; LONGO CESAR PAIXAO, THIAGO REGIS; DE ARAUJO, WILLIAM REIS. Forensic electrochemistry: Electrochemical study and quantification of xylazine in pharmaceutical and urine samples. Electrochimica Acta, v. 295, p. 726-734, FEB 1 2019. (17/10522-5, 15/08539-1, 16/16477-9, 12/06642-1)

FERREIRA, PATRICK CESAR; ATAIDE, VANESSA NEIVA; SILVA CHAGAS, CYRO LUCAS; ANGNES, LUCIO; TOMAZELLI COLTRO, WENDELL KARLOS; LONGO CESAR PAIXAO, THIAGO REGIS; DE ARAUJO, WILLIAM REIS. Wearable electrochemical sensors for forensic and clinical applications. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 119, OCT 2019. (18/14462-0, 17/10522-5, 16/16477-9, 18/08782-1)

DIAS, ANDERSON A.; CHAGAS, CYRO L. S.; SILVA-NETO, HABDIAS DE A.; LOBO-JUNIOR, EULICIO O.; SGOBBI, LIVIA F.; DE ARAUJO, WILLIAM R.; PAIXAO, THIAGO R. L. C.; COLTRO, WENDELL K. T.. Environmentally Friendly Manufacturing of Flexible Graphite Electrodes for a Wearable Device Monitoring Zinc in Sweat. ACS APPLIED MATERIALS & INTERFACES, v. 11, n. 43, p. 39484-39492, OCT 30 2019. (17/10522-5, 18/08782-1)

TASIC, NIKOLA; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Biosensing of D-dimer, making the transition from the central hospital laboratory to bedside determination. Talanta, v. 207, JAN 15 2020. (17/10522-5, 18/08782-1, 18/13922-7, 18/14425-7)

SILVA, THALITA G.; PAIXAO, THIAGO R. L. C.; IEEE. Development of a colorimetric array to discriminate cutting agents in seized cocaine samples. 2019 IEEE INTERNATIONAL SYMPOSIUM ON OLFACTION AND ELECTRONIC NOSE (ISOEN 2019), v. N/A, p. 3-pg., 2019-01-01. (17/10522-5)

BOSSARD, BRUNA; GROTHE, RENATA ANTUNES; MARTINS, ALISSON BEZERRA; LOBATO, ALNILAN; TASIC, NIKOLA; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Nanographene laser-pyrolyzed paper electrodes for the impedimetric detection of D-glucose via a molecularly imprinted polymer. MONATSHEFTE FUR CHEMIE, v. 153, n. 12, p. 7-pg., 2022-10-14. (18/14425-7, 17/10522-5, 18/13922-7, 18/08782-1, 19/11214-8)

ARANTES, IANA V. S.; PAIXAO, THIAGO R. L. C.. ouple batch-injection analysis and microfluidic paper-based analytical device: A simple and disposable alternative to conventional BIA apparatu. Talanta, v. 240, APR 1 2022. (17/10522-5, 19/15065-7, 18/08782-1)

CARDOSO, RAFAEL M.; CASTRO, SILVIA V. F.; SILVA, MURILO N. T.; LIMA, ANA P.; SANTANA, MARIO H. P.; NOSSOL, EDSON; SILVA, RODRIGO A. B.; RICHTER, EDUARDO M.; PAIXAO, THIAGO R. L. C.; MUNOZ, RODRIGO A. A.. 3D-printed flexible device combining sampling and detection of explosives. SENSORS AND ACTUATORS B-CHEMICAL, v. 292, p. 308-313, AUG 1 2019. (17/10522-5)

DE ARAUJO, WILLIAM R.; FRASSON, CAROLINA M. R.; AMEKU, WILSON A.; SILVA, JOSE R.; ANGNES, LUCIO; PAIXAO, THIAGO R. L. C.. Single-Step Reagentless Laser Scribing Fabrication of Electrochemical Paper-Based Analytical Devices. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, v. 56, n. 47, p. 15113-15117, NOV 20 2017. (17/10522-5, 16/16477-9)

FERREIRA SANTOS, MAURO SERGIO; AMEKU, WILSON AKIRA; ROLF GUTZ, IVANO GEBHARDT; LONGO CESAR PAIXAO, THIAGO REGIS. Gold leaf: From gilding to the fabrication of disposable, wearable and low-cost electrodes. Talanta, v. 179, p. 507-511, MAR 1 2018. (17/10522-5)

FERREIRA E SILVA, RAFAEL; LONGO CESAR PAIXAO, THIAGO REGIS; TOROSSIAN TORRES, MARCELO DER; DE ARAUJO, WILLIAM REIS. Simple and inexpensive electrochemical paper-based analytical device for sensitive detection of Pseudomonas aeruginosa. SENSORS AND ACTUATORS B-CHEMICAL, v. 308, APR 1 2020. (17/11398-6, 17/10522-5, 18/08782-1, 16/16477-9)

SPOSITO, HEITOR GABRIEL MARTINS; LOBATO, ALNILAN; TASIC, NIKOLA O.; MALDANER, ADRIANO O.; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Swift electrochemical sensing of diltiazem employing highly-selective molecularly-imprinted 3-amino-4-hydroxybenzoic acid. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 911, p. 7-pg., 2022-03-11. (18/13922-7, 18/14425-7, 19/16491-0, 19/11214-8, 17/10522-5, 18/08782-1, 20/04268-1)

DE CASTRO, LUCAS F.; DE FREITAS, SORAIA V.; DUARTE, LUCAS C.; DE SOUZA, JOAO ANTONIO C.; PAIXAO, THIAGO R. L. C.; COLTRO, WENDELL K. T.. Salivary diagnostics on paper microfluidic devices and their use as wearable sensors for glucose monitoring. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 411, n. 19, SI, p. 4919-4928, JUL 2019. (17/10522-5)

SILVA, THALITA GUEDES; LONGO CESAR DA PAIXAO, THIAGO REGIS. Development and Evaluation of Two Different Electronic Tongues Aiming to the Discrimination of Cutting Agents Found in Cocaine Seized Samples. BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY, v. 9, n. 34, p. 10-pg., 2022-01-01. (18/08782-1, 19/16491-0, 17/10522-5)

AMEKU, WILSON A.; GONCALVES, JOSUE M.; ATAIDE, VANESSA N.; SANTOS, MAURO S. FERREIRA; GUTZ, IVANO G. R.; ARAKI, KOITI; PAIXAO, THIAGO R. L. C.. Combined Colorimetric and Electrochemical Measurement Paper-Based Device for Chemometric Proof-of-Concept Analysis of Cocaine Samples. ACS OMEGA, v. 6, n. 1, p. 594-605, JAN 12 2021. (17/10522-5, 19/16491-0, 16/21070-5, 18/08782-1)

MARTINS, ALISSON BEZERRA; LOBATO, ALNILAN; TASIC, NIKOLA; PEREZ-SANZ, FERNANDO J.; VIDINHA, PEDRO; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Laser-pyrolyzed electrochemical paper-based analytical sensor for sulphite analysis. Electrochemistry Communications, v. 107, OCT 2019. (14/50279-4, 18/20745-4, 19/11214-8, 18/13922-7, 17/10522-5, 18/14425-7)

TASIC, NIKOLA; CAVALCANTE, LETICIA; DEFFUNE, ELENICE; GOES, MARCIO SOUSA; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Probeless and label-free impedimetric biosensing of D-dimer using gold nanoparticles conjugated with dihexadecylphosphate on screen-printed carbon electrodes. Electrochimica Acta, v. 397, NOV 20 2021. (18/13922-7, 18/08782-1, 17/10522-5, 18/14425-7)

DE OLIVEIRA, LUIZA PEREIRA; ROCHA, DIEGO PESSOA; DE ARAUJO, WILLIAM REIS; ABARZA MUNOZ, RODRIGO ALEJANDRO; LONGO CESAR PAIXAO, THIAGO REGIS; SALLES, MAIARA OLIVEIRA. Forensics in hand: new trends in forensic devices (2013-2017). ANALYTICAL METHODS, v. 10, n. 43, NOV 21 2018. (17/10522-5, 16/16477-9)

DE ARAUJO, WILLIAM R.; CARDOSO, THIAGO M. G.; DA ROCHA, RAQUEL G.; SANTANA, MARIO H. P.; MUNOZ, RODRIGO A. A.; RICHTER, EDUARDO M.; PAIXAO, THIAGO R. L. C.; COLTRO, WENDELL K. T.. Portable analytical platforms for forensic chemistry: A review. Analytica Chimica Acta, v. 1034, p. 1-21, NOV 30 2018. (17/10522-5, 16/16477-9)

DA SILVA, GABRIELA O.; DE ARAUJO, WILLIAM R.; PAIXAO, THIAGO R. L. C.. Portable and low-cost colorimetric office paper-based device for phenacetin detection in seized cocaine samples. Talanta, v. 176, p. 674-678, JAN 1 2018. (17/10522-5, 11/19903-5, 16/16477-9)

MENDES, LETICIA FRANCINE; DE SIERVO, ABNER; DE ARAUJO, WILLIAM REIS; LONGO CESAR PAIXAO, THIAGO REGIS. Reagentless fabrication of a porous graphene-like electrochemical device from phenolic paper using laser-scribing. Carbon, v. 159, p. 110-118, APR 15 2020. (17/10522-5, 18/08782-1, 07/54829-5, 18/16250-0)

ATAIDE, VANESSA N.; MENDES, LETICIA F.; GAMA, LILLIA I. L. M.; DE ARAUJO, WILLIAM R.; PAIXAO, THIAGO R. L. C.. Electrochemical paper-based analytical devices: ten years of development. ANALYTICAL METHODS, v. 12, n. 8, p. 1030-1054, FEB 28 2020. (17/10522-5, 18/14462-0, 18/08782-1, 16/16477-9, 19/16491-0)

ATAIDE, VANESSA N.; AMEKU, WILSON A.; BACIL, RAPHAEL R.; ANGNES, LUCIO; DE ARAUJO, WILLIAM R.; PAIXAO, THIAGO R. L. C.. Enhanced performance of pencil-drawn paper-based electrodes by laser-scribing treatment. RSC ADVANCES, v. 11, n. 3, p. 1644-1653, JAN 13 2021. (18/14462-0, 16/21070-5, 18/08782-1, 16/16477-9, 17/10522-5)

TASIC, NIKOLA; MARTINS, ALISSON BEZERRA; YIFEI, XUE; GOES, MARCIO SOUSA; MARTIN-YERGA, DANIEL; MAO, LANQUN; PAIXAO, THIAGO R. L. C.; GONCALVES, LUIS MOREIRA. Insights into electrochemical behavior in laser-scribed electrochemical paper-based analytical devices. Electrochemistry Communications, v. 121, DEC 2020. (18/20745-4, 17/10522-5, 18/13922-7, 18/14425-7)

AMEKU, WILSON A.; DE ARAUJO, WILLIAM R.; RANGEL, CLARA J.; ANDO, ROMULO A.; PAIXAO, THIAGO R. L. C.. Gold Nanoparticle Paper-Based Dual-Detection Device for Forensics Applications. ACS APPLIED NANO MATERIALS, v. 2, n. 9, p. 5460-5468, SEP 2019. (16/21070-5, 17/10522-5, 18/08782-1, 16/16477-9)

DE CASTRO, LUCAS F.; DE FREITAS, SORAIA V.; DUARTE, LUCAS C.; DE SOUZA, JOAO ANTONIO C.; PAIXAO, THIAGO R. L. C.; COLTRO, WENDELL K. T.. Salivary diagnostics on paper microfluidic devices and their use as wearable sensors for glucose monitoring. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 411, n. 19, p. 10-pg., 2019-07-01. (17/10522-5)