Adsorption of bisphosphonates and proteins on TiO2 surfaces for bone implants

• Grant number: 17/15035-5
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2017
• End date: September 30, 2019
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Paulo Noronha Lisboa Filho
• Grantee: Paulo Noronha Lisboa Filho
• Host Institution: Faculdade de Ciências (FC). Universidade Estadual Paulista (UNESP). Campus de Bauru. Bauru , SP, Brazil
• City of the host institution: Bauru
• Associated researchers: Erika Soares Bronze-Uhle ; Rodrigo Cardoso de Oliveira

Abstract

Nanostructured titanium dioxide (TiO2) has been used as surface modifier in biomedical implants, promoting biocorrosion resistance and increasing bioactivity of the oxides, showing promising results with the interaction with living matter. Although the biocompatibility of this oxide is recognized, there are still many aspects of the adhesion mechanisms between biomolecules and the surface of the material there are some open questions that must need to be better understood. Chemically the surface of these oxides is mostly terminated by OH groups, which can be readily functionalized with bifunctional organic molecules. This chemical functionalization or simply a physical modification of the surface can improve the interaction of the oxide with the biological environment, improving bone resorption in orthopedic and dental implants.This project proposes to synthesize TiO2 thin films on metallic titanium substrates and to study different functionalization strategies of surfaces using biofunctional molecules such as bisphosphonates and proteins. It is also intended to evaluate the physico-chemical and thermodynamic parameters in order to analyze the better interaction and adsorption between protein / bisphosphonate and TiO2 surface, since different biomedical applications require different protein-oxide interaction properties. Different strategies of functionalization and adsorption will be studied and tested in biological systems in vitro. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALBANO, CAROLINA SIMAO; GOMES, ANDERSON MOREIRA; FELTRAN, GEORGIA DA SILVA; DA COSTA FERNANDES, CELIO JUNIOR; TRINO, LUCIANA DANIELE; ZAMBUZZI, WILLIAN FERNANDO; LISBOA-FILHO, PAULO NORONHA. Biofunctionalization of titanium surfaces with alendronate and albumin modulates osteoblast performance. HELIYON, v. 6, n. 7, JUL 2020. (17/02366-3, 14/22689-3, 17/15035-5)

ALBANO, CAROLINA SIMAO; GOMES, ANDERSON MOREIRA; FELTRAN, GEORGIA DA SILVA; DA COSTA FERNANDES, CELIO JUNIOR; TRINO, LUCIANA DANIELE; ZAMBUZZI, WILLIAN FERNANDO; LISBOA-FILHO, PAULO NORONHA. Bisphosphonate-based surface biofunctionalization improves titanium biocompatibility. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 31, n. 11, NOV 7 2020. (17/02366-3, 17/15035-5, 14/22689-3)

GOMES, O. P.; FELTRAN, G. S.; FERREIRA, M. R.; ALBANO, C. S.; ZAMBUZZI, W. F.; LISBOA-FILHO, P. N.. A novel BSA immobilizing manner on modified titanium surface ameliorates osteoblast performance. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 190, JUN 2020. (16/22186-7, 14/22689-3, 17/15035-5, 13/07296-2, 14/20471-0)

BRONZE-UHLE, ERIKA S.; DIAS, LEONARDO F. G.; TRINO, LUCIANA D.; MATOS, ADRIANA A.; DE OLIVEIRA, RODRIGO C.; LISBOA-FILHO, PAULO N.. Physicochemical characterization of albumin immobilized on different TiO2 surfaces for use in implant materials. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, v. 564, p. 39-50, MAR 5 2019. (14/01713-3, 14/20471-0, 16/11183-7, 17/15035-5)

BRONZE-UHLE, ERIKA S.; DIAS, LEONARDO F. G.; TRINO, LUCIANA D.; MATOS, ADRIANA A.; DE OLIVEIRA, RODRIGO C.; LISBOA-FILHO, PAULO N.. Physicochemical bisphosphonate immobilization on titanium dioxide thin films surface by UV radiation for bio-application. SURFACE & COATINGS TECHNOLOGY, v. 357, p. 36-47, JAN 15 2019. (14/01713-3, 14/20471-0, 16/11183-7, 17/15035-5)

KITAGAWA, IGOR L.; MIYAZAKI, CELINA M.; PITOL-PALIN, LETICIA; OKAMOTO, ROBERTA; DE VASCONCELLOS, LUANA M. R.; CONSTANTINO, CARLOS J. L.; LISBOA-FILHO, PAULO N.. Titanium-Based Alloy Surface Modification with TiO2 and Poly(sodium 4-styrenesulfonate) Multilayers for Dental Implants. ACS APPLIED BIO MATERIALS, v. 4, n. 4, p. 3055-3066, APR 19 2021. (14/15093-7, 17/15035-5, 18/03114-0, 13/07296-2)

TRINO, LUCIANA D.; ALBANO, LUIZ G. S.; BRONZE-UHLE, ERIKA S.; GEORGE, ANNE; MATHEW, MATHEW T.; LISBOA-FILHO, PAULO N.. Physicochemical, osteogenic and corrosion properties of bio-functionalized ZnO thin films: Potential material for biomedical applications. CERAMICS INTERNATIONAL, v. 44, n. 17, p. 21004-21014, DEC 1 2018. (14/01713-3, 13/07296-2, 13/09963-6, 14/27015-0, 17/15035-5)