Analyses of the neuromuscular junctions and associated proteins with focus on morphofunctional recovery: a comparative use of fibrin sealant and suture.

• Grant number: 17/06472-2
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2018
• End date: January 31, 2020
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Selma Maria Michelin Matheus
• Grantee: Selma Maria Michelin Matheus
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: André Luis Filadelpho ; Antonio de Castro Rodrigues ; Carina Guidi Pinto ; Rui Seabra Ferreira Junior

Abstract

Peripheral nerves are often affected by injuries that lead to nerve impulse interruption. Functional recovery is usually late and considered a challenge for medical practice. Thus, we suggest that this type of response is associated with failures in the reinnervation of the neuromuscular junction (NMJ), accompanied by alterations in its structural proteins, causing a defect in the muscular eletric activation capacity. This project aims to compare the use of fibrin sealant and suture after sciatic nerve injury with focus on the NMJ and associated proteins. Fifty adult male Wistar rats will be used, divided into five experimental groups: Control-Sham (CS) and Control-dennervated (CD); Suture Injury (SI); Fibrin Sealant Injury (FI) and Suture Injury + Fibrin Sealant (FSI). The animals (preoperative) of CS will be submitted to a superficial incision of the skin after the catwalk anlysis. The right sciatic nerve neurotmesis will be performed in the CD, SI, FI and FSI groups.It will be associated with proximal and distal stump fixation in the adjacent muscles. After 7 days it will be done the reconnection of the stumps with fibrin sealant (CEVAP) in the FI and with suture in the SI and both procedures in the FSI. At 15, 30 and 60 days the animals will undergo a catwalk test again and they will be euthanized at the end of the experiment (60 days). The right ischiatic nerves will be removed and analyzed for morphologic and morphometric parameters. The right soleus muscles will be processed to analyse NMJ morphology, ultrastructure and morphometry; nAChRs morphometry and nerve terminals morphology by confocal microscopy; morphology and morphometry of muscle fibers (HE), I and II fibers types frequency, and Protein quantification of nAChRs, Agrina, Musk MMP3 and Schwann cells. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TIBURCIO, FELIPE CANTORE; MULLER, KEVIN SILVA; LEITE, ANA PAULA SILVEIRA; DE OLIVEIRA, IGOR RODRIGUES ARAUJO; BARRAVIERA, BENEDITO; FERREIRA JR, RUI SEABRA; PADOVANI, CARLOS ROBERTO; PINTO, CARINA GUIDI; MATHEUS, SELMA MARIA MICHELIN. Neuroregeneration and immune response after neurorrhaphy are improved with the use of heterologous fibrin biopolymer in addition to suture repair alone. MUSCLE & NERVE, v. N/A, p. 15-pg., 2023-03-25. (17/06472-2)

PINTO, CARINA GUIDI; SILVEIRA LEITE, ANA PAULA; SARTORI, ARTHUR ALVES; TIBURCIO, FELIPE CANTORE; BARRAVIERA, BENEDITO; FERREIRA JUNIOR, RUI SEABRA; FILADELPHO, ANDRE LUIS; DE CARVALHO, SAMARA CAMACARI; MICHELIN MATHEUS, SELMA MARIA. Heterologous fibrin biopolymer associated to a single suture stitch enables the return of neuromuscular junction to its mature pattern after peripheral nerve injury. INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED, v. 52, n. 4, p. 7-pg., 2021-04-22. (17/06472-2)

SILVEIRA LEITE, ANA PAULA; PINTO, CARINA GUIDI; TIBURCIO, FELIPE CANTORE; SARTORI, ARTHUR ALVES; RODRIGUES, ANTONIO DE CASTRO; BARRAVIERA, BENEDITO; FERREIRA JUNIOR, RUI SEABRA; FILADELPHO, ANDRE LUIS; MICHELIN MATHEUS, SELMA MARIA. Heterologous fibrin sealant potentiates axonal regeneration after peripheral nerve injury with reduction in the number of suture points. INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED, v. 50, n. 4, p. 834-847, APR 2019. (17/06472-2)