Molecular characterization of cyanobacteria strains isolated from São Paulo State mangrove regions

• Grant number: 06/01671-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: January 01, 2007
• End date: June 30, 2010
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Marli de Fátima Fiore
• Grantee: Caroline Souza Pamplona da Silva
• Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil

Abstract

This research project aims to molecular characterize cyanobacterial strains isolated from mangroves regions of the São Paulo State by sequencing their 16S rRNA gene, which has been used to differentiate genetically microorganisms belonging to the Bacteria domain. The sequences obtained will be compared by BLAST (NCBI) analysis and the evolutionary distance between the sequenced nucleotides strains will be analyzed by phylogenetic tree construction. At least fifty cyanobacterial strains will be evaluated. The PCR amplification of sequences of nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzymes genes will allow to identify among the molecular characterized strains those potentially producing of biochemically active natural compound (cyanopeptides). These enzymes are known to be involved in toxins and antibiotics biosynthesis. The detection of these nonribosomal peptides genes will be carried out using degenerate oligonucleotides primers target to conserved sequences of NRPS and PKS enzymes. The PCR products obtained for these enzymes will be sequenced. These sequences also will be compared by BLAST analysis and their evolutionary relations will be verified by phylogenetic tree construction. The cyanopeptides produced by the strains containing NRPS and PKS genes will be extracted using several solvents. The chemical constituent of cellular extracts will be analyzed using Q-TOF-MS (Quadrupole Time-of-Flight Mass Spectrometry) and HPLC (High Performance Liquid Chromatography). The inhibitory activity of these chemical compounds will be tested against fungus and bacteria.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SILVA-STENICO, MARIA ESTELA; PAMPLONA SILVA, CAROLINE SOUZA; LORENZI, ADRIANA STURION; SHISHIDO, TANIA KEIKO; ETCHEGARAY, AUGUSTO; LIRA, SIMONE POSSEDENTE; BERALDO MORAES, LUIZ ALBERTO; FIORE, MARLI FATIMA. Non-ribosomal peptides produced by Brazilian cyanobacterial isolates with antimicrobial activity. MICROBIOLOGICAL RESEARCH, v. 166, n. 3, p. 161-175, 2011. (06/01671-2, 09/13455-0)

SILVA-STENICO, MARIA ESTELA; VIEIRA, FELIPE D. P.; GENUARIO, DIEGO B.; SILVA, CAROLINE S. P.; MORAES, LUIZ ALBERTO B.; FIORE, MARLI FATIMA. Decolorization of textile dyes by cyanobacteria. Journal of the Brazilian Chemical Society, v. 23, n. 10, p. 1863-1870, OCT 2012. (06/01671-2, 10/09867-9, 10/00321-3, 09/05474-5)

SILVA-STENICO, MARIA ESTELA; LORENZI, ADRIANA STURION; TESCHKE, OMAR; PAMPLONA SILVA, CAROLINE SOUZA; ETCHEGARAY, AUGUSTO; FIORE, MARLI FATIMA. Antimicrobial Cyanopeptide Action on Bacterial Cells Observed with Atomic Force Microscopy. CURRENT NANOSCIENCE, v. 9, n. 1, p. 141-148, FEB 2013. (06/01671-2, 09/05474-5, 10/09867-9, 03/12529-4)

FIORE, MARLI FATIMA; GENUARIO, DIEGO BONALDO; PAMPLONA DA SILVA, CAROLINE SOUZA; SHISHIDO, TANIA KEIKO; BERALDO MORAES, LUIZ ALBERTO; CANTUSIO NETO, ROMEU; SILVA-STENICO, MARIA ESTELA. Microcystin production by a freshwater spring cyanobacterium of the genus Fischerella. Toxicon, v. 53, n. 7-8, p. 754-761, JUN 2009. (06/01671-2, 07/06360-8)

PAMPLONA SILVA, CAROLINE SOUZA; GENUARIO, DIEGO BONALDO; MARCAL VIEIRA VAZ, MARCELO GOMES; FIORE, MARLI FATIMA. Phylogeny of culturable cyanobacteria from Brazilian mangroves. Systematic and Applied Microbiology, v. 37, n. 2, p. 100-112, MAR 2014. (06/01671-2, 10/18732-0, 10/00321-3, 04/13910-6)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SILVA, Caroline Souza Pamplona da. Molecular characterization of cyanobacteria isolated from mangrove ecosystem of the State of São Paulo and identification of natural products. 2010. Doctoral Thesis - Universidade de São Paulo (USP). Escola Superior de Agricultura Luiz de Queiroz (ESALA/BC) Piracicaba.